Alignments for a candidate for galE in Flavobacterium sp. LM5

GapMind for catabolism of small carbon sources

Alignments for a candidate for galE in Flavobacterium sp. LM5

Align UDP-glucose 4-epimerase; EC 5.1.3.2 (characterized, see rationale)
to candidate WP_024981387.1 BXU11_RS03860 UDP-glucose 4-epimerase GalE

Query= uniprot:L0FXG3
         (337 letters)



>NCBI__GCF_002017945.1:WP_024981387.1
          Length = 337

 Score =  363 bits (933), Expect = e-105
 Identities = 177/336 (52%), Positives = 244/336 (72%), Gaps = 3/336 (0%)

Query: 3   QILITGGAGYIGSHTAVALVNAGYEPIIVDNFSNSNKEVLNGLEKILGAPVKYHEGDCND 62
           +IL+TGG G+IGSHT V L N G+E +IVDN  NS+ +VL  +E I G    Y   D  D
Sbjct: 2   KILVTGGLGFIGSHTVVELQNKGFEVLIVDNLYNSSIDVLQRIESISGIAPHYKNIDMRD 61

Query: 63  RKFMQAVFEE-NDLQGVIHFAASKAVGESTKIPLTYYSNNINSLIILLETMKQFGVKDIV 121
           +  +QA+F+E +D+ GVIHFAASKAVGES + PL YY NNI +L+ LL+ ++Q      +
Sbjct: 62  KAAVQALFKEYSDIAGVIHFAASKAVGESVENPLLYYENNIAALVYLLQELQQKDAASFI 121

Query: 122 FSSSCTVYGQPDKLPVKETTPRKDAESPYGNTKKICEDILTDHVKSGAPARVVALRYFNP 181
           FSSSCTVYGQ +K+P+ E  P +   SPYGNTKK+ E+I+ D  K+ +    + LRYFNP
Sbjct: 122 FSSSCTVYGQAEKMPITEDAPVQQPMSPYGNTKKVGEEIIRDTAKASS-INAILLRYFNP 180

Query: 182 IGAHPSSLIGELPLGVPANLIPFVTQTGAGIREKITVFGDDYNTPDGTCIRDYIHVMDLA 241
           IGAHPS+ IGELP+GVP NL+PF+TQTG G+R++++VFG+DY TPDGT +RDYIHV+DLA
Sbjct: 181 IGAHPSAEIGELPIGVPQNLVPFITQTGIGLRQELSVFGNDYPTPDGTAVRDYIHVVDLA 240

Query: 242 DAHVKSIQYLADQPE-NFIDLFNVGTGNGNTVMEVIKAFEKVSEKPLNYEIGPRRSGDIE 300
            AHV +++ L D+     ++ FN+GTG G++V+EVI++FE+VS + L Y+I PRR GDI 
Sbjct: 241 KAHVIALERLLDKKNTEKVETFNLGTGKGSSVLEVIQSFERVSGQKLPYKIMPRREGDIT 300

Query: 301 KVWANTDKVSKVLGWTPQFGLEEALRDAWNWQLSLK 336
           + +ANT K + VLGW  +  L+EA+  AW W+  ++
Sbjct: 301 EAYANTHKANTVLGWKAESTLDEAMASAWKWEQKIR 336


Lambda     K      H
   0.316    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 366
Number of extensions: 10
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 337
Length of database: 337
Length adjustment: 28
Effective length of query: 309
Effective length of database: 309
Effective search space:    95481
Effective search space used:    95481
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_024981387.1 BXU11_RS03860 (UDP-glucose 4-epimerase GalE)
to HMM TIGR01179 (galE: UDP-glucose 4-epimerase GalE (EC 5.1.3.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01179.hmm
# target sequence database:        /tmp/gapView.1699719.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01179  [M=332]
Accession:   TIGR01179
Description: galE: UDP-glucose 4-epimerase GalE
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.4e-125  404.5   0.0   1.5e-125  404.4   0.0    1.0  1  NCBI__GCF_002017945.1:WP_024981387.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_002017945.1:WP_024981387.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  404.4   0.0  1.5e-125  1.5e-125       1     328 [.       2     335 ..       2     337 .] 0.97

  Alignments for each domain:
  == domain 1  score: 404.4 bits;  conditional E-value: 1.5e-125
                             TIGR01179   1 kiLvtGgaGyiGshvvrqllekgkevvvlDnlskgskealkalekit..evklvegdladkekleavlee.ek 70 
                                           kiLvtGg G+iGsh+v++l++kg+ev+++Dnl ++s ++l+++e i+  + ++ + d++dk++++a+++e ++
  NCBI__GCF_002017945.1:WP_024981387.1   2 KILVTGGLGFIGSHTVVELQNKGFEVLIVDNLYNSSIDVLQRIESISgiAPHYKNIDMRDKAAVQALFKEySD 74 
                                           79********************************************9888889999************97258 PP

                             TIGR01179  71 idaviHfaaliavgEsvkePlkYYennvvntleLleamqkagvkkliFsssaavYgesekvpisEespln.pi 142
                                           i  viHfaa++avgEsv++Pl YYenn+ + + Ll+++q+    ++iFsss++vYg+ ek+pi+E++p++ p+
  NCBI__GCF_002017945.1:WP_024981387.1  75 IAGVIHFAASKAVGESVENPLLYYENNIAALVYLLQELQQKDAASFIFSSSCTVYGQAEKMPITEDAPVQqPM 147
                                           99******************************************************************999** PP

                             TIGR01179 143 npYGrsklmvErilkdlkkadkelkvviLRYFnvaGAdeegeiGeasknat.hliklvaevavgkrekleifG 214
                                           +pYG++k + E+i++d++ka ++++ ++LRYFn++GA+++ eiGe + + + +l++ ++++ +g r++l++fG
  NCBI__GCF_002017945.1:WP_024981387.1 148 SPYGNTKKVGEEIIRDTAKA-SSINAILLRYFNPIGAHPSAEIGELPIGVPqNLVPFITQTGIGLRQELSVFG 219
                                           ********************.9*****************************9********************* PP

                             TIGR01179 215 tdyptkDGtcvRDyiHveDlaeaHlaalealeeg...gesevynlGagqgfsvkevieavkkvsgkdikvela 284
                                           +dypt+DGt+vRDyiHv Dla+aH+ ale l ++   +++e++nlG+g+g sv+evi+ +++vsg+++++++ 
  NCBI__GCF_002017945.1:WP_024981387.1 220 NDYPTPDGTAVRDYIHVVDLAKAHVIALERLLDKkntEKVETFNLGTGKGSSVLEVIQSFERVSGQKLPYKIM 292
                                           *****************************9976633367899******************************* PP

                             TIGR01179 285 drRaGDpaslvadaskikrelgwkpkyddLeeiiksawdWekkl 328
                                           +rR+GD+++ +a+++k++++lgwk++ + L+e ++saw+We+k+
  NCBI__GCF_002017945.1:WP_024981387.1 293 PRREGDITEAYANTHKANTVLGWKAEST-LDEAMASAWKWEQKI 335
                                           ***************************9.************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (332 nodes)
Target sequences:                          1  (337 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 24.58
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources