GapMind for catabolism of small carbon sources

 

Alignments for a candidate for AZOBR_RS08250 in Flavobacterium sp. LM5

Align Leucine//isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate WP_024982395.1 BXU11_RS05265 LPS export ABC transporter ATP-binding protein

Query= uniprot:G8ALJ1
         (236 letters)



>NCBI__GCF_002017945.1:WP_024982395.1
          Length = 250

 Score =  116 bits (290), Expect = 5e-31
 Identities = 70/235 (29%), Positives = 122/235 (51%), Gaps = 8/235 (3%)

Query: 2   LKVSGVHTFYGAIEALKGVDIEIGAGEIVSLIGANGAGKSTLLMTICGSPRARMGRITFE 61
           L+   +   Y     +KG+ +E+  GEIV L+G NGAGK+T    I G  +   G I  +
Sbjct: 3   LRAENLIKTYKGRSVVKGISLEVNQGEIVGLLGPNGAGKTTSFYMIVGLVKPNSGHIYLD 62

Query: 62  GQDITQMPTYELVRLGIAQSPEGRRIFPRMSVLEN----LQMGSITAKPGSFANELERVL 117
             DITQ P Y+  + G+    +   +F ++S+ +N    LQ+ + T +      ++E ++
Sbjct: 63  DLDITQYPMYKRAQQGVGYLAQEASVFRKLSIEDNILSVLQLTNHTKE--EQVAKMESLI 120

Query: 118 TLFPRLKERISQRAGTMSGGEQQMLAIGRALMSQPRLLLLDEPSLGLAPLVVKQIFQAVK 177
             F  L+   + R   +SGGE++   I R L + P+ +LLDEP  G+ P+ V+ I + V 
Sbjct: 121 AEF-SLEHIRTNRGDLLSGGERRRTEIARCLATDPKFILLDEPFAGVDPVAVEDIQRIVA 179

Query: 178 DINREQKMTVFMVEQNAFHALKLAHRGYVMVNGKVTMSGTGAELLANEEVRSAYL 232
            + + + + + + + N    L +  + Y+M  G +  +GT  EL+ +E VR  YL
Sbjct: 180 QL-KNKNIGILITDHNVQETLAITDKTYLMFEGGILKAGTPEELVEDEMVRRVYL 233


Lambda     K      H
   0.320    0.136    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 123
Number of extensions: 7
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 236
Length of database: 250
Length adjustment: 23
Effective length of query: 213
Effective length of database: 227
Effective search space:    48351
Effective search space used:    48351
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory