Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate WP_024982395.1 BXU11_RS05265 LPS export ABC transporter ATP-binding protein
Query= uniprot:A0A165KC78 (242 letters) >NCBI__GCF_002017945.1:WP_024982395.1 Length = 250 Score = 125 bits (313), Expect = 1e-33 Identities = 73/237 (30%), Positives = 125/237 (52%), Gaps = 8/237 (3%) Query: 10 LQVKGLKVAYGGIQAVKGVDFEVREGELVSLIGSNGAGKTTTMKAITGTLSMNDGNIEYL 69 L+ + L Y G VKG+ EV +GE+V L+G NGAGKTT+ I G + N G+I Sbjct: 3 LRAENLIKTYKGRSVVKGISLEVNQGEIVGLLGPNGAGKTTSFYMIVGLVKPNSGHIYLD 62 Query: 70 GKSIKGKGAWDLVKEGLVMVPEGRGVFARMTITEN----LQMGAYIRKDKAGILADIEKM 125 I + ++G+ + + VF +++I +N LQ+ + ++++ + + Sbjct: 63 DLDITQYPMYKRAQQGVGYLAQEASVFRKLSIEDNILSVLQLTNHTKEEQVAKMESLIAE 122 Query: 126 FTIFPRLRERKDQLAGTMSGGEQQMLAMGRALMSQPKVLLLDEPSMGLSPIMVDKIFEVV 185 F++ +R + L +SGGE++ + R L + PK +LLDEP G+ P+ V+ I +V Sbjct: 123 FSL-EHIRTNRGDL---LSGGERRRTEIARCLATDPKFILLDEPFAGVDPVAVEDIQRIV 178 Query: 186 RDVYALGVTIVLVEQNASRALAIADRGYVMESGLITMTGPGQQLLNDPKVRAAYLGE 242 + + I++ + N LAI D+ Y+M G I G ++L+ D VR YLG+ Sbjct: 179 AQLKNKNIGILITDHNVQETLAITDKTYLMFEGGILKAGTPEELVEDEMVRRVYLGQ 235 Lambda K H 0.317 0.136 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 146 Number of extensions: 13 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 242 Length of database: 250 Length adjustment: 24 Effective length of query: 218 Effective length of database: 226 Effective search space: 49268 Effective search space used: 49268 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory