GapMind for catabolism of small carbon sources

 

Protein WP_084934563.1 in Pantoea rwandensis LMG 26275

Annotation: NCBI__GCF_002095475.1:WP_084934563.1

Length: 709 amino acids

Source: GCF_002095475.1 in NCBI

Candidate for 34 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism fadB hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
4-hydroxybenzoate catabolism paaH hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
L-arginine catabolism fadB hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
L-citrulline catabolism fadB hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
L-isoleucine catabolism ech hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2
L-lysine catabolism fadB hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
phenylacetate catabolism fadB hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
phenylacetate catabolism paaH hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
L-phenylalanine catabolism fadB hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
L-phenylalanine catabolism paaH hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
L-proline catabolism fadB hi fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 66% 99% 932.2 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 42% 536.2
L-isoleucine catabolism fadA med long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211); acetyl-CoA C-acyltransferase (EC 2.3.1.16) (characterized) 43% 94% 536.2 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
4-hydroxybenzoate catabolism ech med crotonase (EC 4.2.1.150) (characterized) 41% 71% 123.2
L-arginine catabolism ech med crotonase (EC 4.2.1.150) (characterized) 41% 71% 123.2
L-citrulline catabolism ech med crotonase (EC 4.2.1.150) (characterized) 41% 71% 123.2
L-lysine catabolism ech med crotonase (EC 4.2.1.150) (characterized) 41% 71% 123.2
phenylacetate catabolism ech med crotonase (EC 4.2.1.150) (characterized) 41% 71% 123.2
L-phenylalanine catabolism ech med crotonase (EC 4.2.1.150) (characterized) 41% 71% 123.2
L-proline catabolism ech med crotonase (EC 4.2.1.150) (characterized) 41% 71% 123.2
L-valine catabolism ech med crotonase (EC 4.2.1.150) (characterized) 41% 71% 123.2
4-hydroxybenzoate catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 34% 97% 346.7 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
phenylacetate catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 34% 97% 346.7 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
L-phenylalanine catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 34% 97% 346.7 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
L-isoleucine catabolism hpcD lo 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 43% 66% 139.8 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
propionate catabolism hpcD lo 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 43% 66% 139.8 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
L-threonine catabolism hpcD lo 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 43% 66% 139.8 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
L-valine catabolism hpcD lo 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 43% 66% 139.8 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
4-hydroxybenzoate catabolism paaF lo enoyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 73% 107.8 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
phenylacetate catabolism paaF lo enoyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 73% 107.8 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
L-phenylalanine catabolism paaF lo enoyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 73% 107.8 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
4-hydroxybenzoate catabolism badK lo BadK (characterized) 33% 83% 104 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
phenylacetate catabolism badK lo BadK (characterized) 33% 83% 104 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
L-phenylalanine catabolism badK lo BadK (characterized) 33% 83% 104 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2
L-leucine catabolism liuC lo methylglutaconyl-CoA hydratase (EC 4.2.1.18) (characterized) 33% 73% 97.4 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 66% 932.2

Sequence Analysis Tools

View WP_084934563.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSPSAFHLQMRLDHVGVITIDVPGEKMNTLRAEFVSQIKAVLAEARSNPQLAGLVLISGK
SDNFVAGADISMIAKCQTAEEAEALARQGQEVMAEIAALPFPVVAAIHGACLGGGLELAL
ACSARICSLDEKTRLGLPEVQLGLLPGSGGTQRLPKLIGVPQALPLILSGKQLRAKQALK
LGVVDDAVPLSILLETAIAQVHKGKVLRRELTLRDRIMQAPMVRSLLFAMAKRQTDSKTQ
GNYPATDRILQVVRIGLEQGSNAGHAAEARAFGQLAMTPESAALRSLFFASTAIKKEAMA
DAEPRPLKHIGVLGGGLMGGGIASVTALKAGSPVRIKDVDLNGVNHALQYSWDLLSQKVK
RRQMKPAERQQQMARISGGTDYQGFSHRDVVVEAVFEDLALKQQMVAEVENHCQPQTIFA
SNTSSLPIGDIAANAQRPENVIGLHYFSPVDKMPLVEVIPHAGTSAATLATTVKLARQQG
KTAIVVADRPGFYVNRILAPYINEAMRCLLEGEPVEHIDRALVKFGFPVGPLQLLDEVGI
DVGSKISPILKQAYGERFSAPEAVNAILADGRKGRKNKKGFYLYTRSRWPGQRKVDDSLY
TLLNIKPLAKQSEKQIAERCVLMMLNEAARCLDEGVIRSARDGDIAAVFGIGFPPFLGGP
FHYMDQRGGLDIVNTLRALMQQQGERFVPCEALMTMSEAQTNFYTSAQS

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory