Alignments for a candidate for pimF in Pantoea rwandensis LMG 26275

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Pantoea rwandensis LMG 26275

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate WP_084934563.1 HA51_RS11045 fatty acid oxidation complex subunit alpha FadJ

Query= metacyc::MONOMER-20678
         (699 letters)



>NCBI__GCF_002095475.1:WP_084934563.1
          Length = 709

 Score =  330 bits (846), Expect = 1e-94
 Identities = 238/715 (33%), Positives = 366/715 (51%), Gaps = 56/715 (7%)

Query: 11  DQVAIVTVDSP--PVNALSAAVRRGILENVNAAVADPAVQAIVLVCA-GRTFIAGADITE 67
           D V ++T+D P   +N L A     I   +  A ++P +  +VL+      F+AGADI+ 
Sbjct: 13  DHVGVITIDVPGEKMNTLRAEFVSQIKAVLAEARSNPQLAGLVLISGKSDNFVAGADISM 72

Query: 68  FGK---PPQPPAL----NDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEAK 120
             K     +  AL     +V+A +   P P +AAIHG  LGGGLE+AL C  R+   + K
Sbjct: 73  IAKCQTAEEAEALARQGQEVMAEIAALPFPVVAAIHGACLGGGLELALACSARICSLDEK 132

Query: 121 --LGLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVVENLV 178
             LGLPEV+LGLLPG+GGTQRLP+ +G   A+ +I+ G  + A +ALK G+V++ V   +
Sbjct: 133 TRLGLPEVQLGLLPGSGGTQRLPKLIGVPQALPLILSGKQLRAKQALKLGVVDDAVPLSI 192

Query: 179 AGAVAFAK----KVLAEKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTKKARGLEAPFA 234
               A A+    KVL  +  LR     D  + A      +F  A      K +G    + 
Sbjct: 193 LLETAIAQVHKGKVLRRELTLR-----DRIMQAPMVRSLLFAMAKRQTDSKTQG---NYP 244

Query: 235 CADAIGAAIDLPFEEGLKK----EREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGT 290
             D I   + +  E+G       E   F +L ++ +S A R  FFA   A K + + D  
Sbjct: 245 ATDRILQVVRIGLEQGSNAGHAAEARAFGQLAMTPESAALRSLFFAST-AIKKEAMADA- 302

Query: 291 KPRPVSRVAIIGAGTMGGGIAMSFA-NAGIPVTLIETGEEQLKRGLGIMQKNWEATAARG 349
           +PRP+  + ++G G MGGGIA   A  AG PV + +     +   L   Q +W+  + + 
Sbjct: 303 EPRPLKHIGVLGGGLMGGGIASVTALKAGSPVRIKDVDLNGVNHAL---QYSWDLLSQKV 359

Query: 350 GLPPDAPAKR---MALITGLVGLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVL 406
                 PA+R   MA I+G    +     D+++EAVFE +A+K+++   V+ H +P  + 
Sbjct: 360 KRRQMKPAERQQQMARISGGTDYQGFSHRDVVVEAVFEDLALKQQMVAEVENHCQPQTIF 419

Query: 407 ASNTSYLSIDEIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKI 466
           ASNTS L I +IAA  +RP++V+G+H+FSP + M L E++  A T+   L T V +A++ 
Sbjct: 420 ASNTSSLPIGDIAANAQRPENVIGLHYFSPVDKMPLVEVIPHAGTSAATLATTVKLARQQ 479

Query: 467 AKVPVVVGVCDGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAG 526
            K  +VV    GF  NR+LA    ++ + L EG   + +D  + KFG P+GP  + D  G
Sbjct: 480 GKTAIVVADRPGFYVNRILAPYINEAMRCLLEGEPVEHIDRALVKFGFPVGPLQLLDEVG 539

Query: 527 LDIGWR-------SRKDRGIKSEIADALCEAGRFGQKTGKGYYKYEQGSRAP---MPDPE 576
           +D+G +       +  +R    E  +A+   GR G+K  KG+Y Y + SR P     D  
Sbjct: 540 IDVGSKISPILKQAYGERFSAPEAVNAILADGRKGRKNKKGFYLYTR-SRWPGQRKVDDS 598

Query: 577 VETLIN-DTLAKLGLKRRDITDEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGY 635
           + TL+N   LAK        ++++I ER V  M+NE AR L+E +     D D+  ++G 
Sbjct: 599 LYTLLNIKPLAKQ-------SEKQIAERCVLMMLNEAARCLDEGVIRSARDGDIAAVFGI 651

Query: 636 GWPIYRGGPMHYADSVGLKHIAERLSAYAKATNDPSLEPAPLLARLAAEGKTFAS 690
           G+P + GGP HY D  G   I   L A  +   +  +    L+    A+   + S
Sbjct: 652 GFPPFLGGPFHYMDQRGGLDIVNTLRALMQQQGERFVPCEALMTMSEAQTNFYTS 706


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1000
Number of extensions: 51
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 709
Length adjustment: 39
Effective length of query: 660
Effective length of database: 670
Effective search space:   442200
Effective search space used:   442200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources