GapMind for catabolism of small carbon sources

 

Alignments for a candidate for braF in Marivita geojedonensis DPG-138

Align High-affinity branched-chain amino acid transport ATP-binding protein BraF, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate WP_085635672.1 MGEO_RS05255 ABC transporter ATP-binding protein

Query= TCDB::P21629
         (255 letters)



>NCBI__GCF_002115805.1:WP_085635672.1
          Length = 245

 Score =  185 bits (469), Expect = 9e-52
 Identities = 106/253 (41%), Positives = 150/253 (59%), Gaps = 20/253 (7%)

Query: 5   ILEVSGLTMRFGGLLAVNGVNLKVEEKQVVSMIGPNGAGKTTVFNCLTGFYQPTGGLIRL 64
           +L + G T RFGGL+AVN ++  V E +V+ +IGPNG+GK+T  N ++G  +PT G I L
Sbjct: 7   VLSLRGATKRFGGLVAVNDMDFDVTEHEVLGLIGPNGSGKSTTLNLISGALKPTEGTISL 66

Query: 65  DGEEIQGLPGHKIARKGVVRTFQNVRLFKEMTAVENL---LVAQHRHLNTNFLAGLFKTP 121
            G  I  L    IAR+G+ RTFQ VRL   MT +EN+    V  HR              
Sbjct: 67  RGTPISHLSAADIARRGIARTFQLVRLLPSMTVLENVRAGAVFGHR-------------- 112

Query: 122 AFRRSEREAMEYAAHWLEEVNLTEFANRSAGTLAYGQQRRLEIARCMMTRPRILMLDEPA 181
             RR   EA  +A   L  V L   AN   G L Y   +R+E+AR + + P++L+LDE  
Sbjct: 113 --RRWGAEADAHAMRMLALVGLDRRANAHVGELTYIDTKRVELARVLASEPKVLLLDEWL 170

Query: 182 AGLNPKETDDLKALIAKLRSEHNVTVLLIEHDMKLVMSISDHIVVINQGAPLADGTPEQI 241
           AGLNP E ++  ALI +LR E   T++L+EH M  + S+ D  VV++ G  +A+GTPE++
Sbjct: 171 AGLNPTELEEGIALIKRLRDEGR-TIILVEHVMDAIRSLCDRCVVMSTGTKIAEGTPEEV 229

Query: 242 RDNPDVIKAYLGE 254
             +P+VI+AYLG+
Sbjct: 230 LSDPEVIRAYLGD 242


Lambda     K      H
   0.320    0.137    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 159
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 245
Length adjustment: 24
Effective length of query: 231
Effective length of database: 221
Effective search space:    51051
Effective search space used:    51051
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory