Align PEB1C, component of Uptake system for glutamate and aspartate (characterized)
to candidate WP_085636165.1 MGEO_RS07775 amino acid ABC transporter ATP-binding protein
Query= TCDB::A3ZI83 (242 letters) >NCBI__GCF_002115805.1:WP_085636165.1 Length = 263 Score = 261 bits (667), Expect = 1e-74 Identities = 132/241 (54%), Positives = 178/241 (73%), Gaps = 2/241 (0%) Query: 1 MIELKNVNKYYGTHHVLKNINLSVKEGEKLVIIGPSGSGKSTTIRCMNGLEEVSSGEVVV 60 +IE++ ++K+YG VL +I+L V+ GE++VI GPSGSGKST IRC+N LEE G +V+ Sbjct: 22 VIEIEKMSKFYGAFQVLHDIDLQVRRGERIVICGPSGSGKSTLIRCLNRLEEHQEGRIVI 81 Query: 61 NNLVLNHKNK-IEICRKYCAMVFQHFNLYPHMTVLQNLTLAPMKLQKKSKKEAEETAFKY 119 + + L K I+ R M FQHFNL+PH+T+L+N TLAPM ++ ++EAE TA ++ Sbjct: 82 DGVELTSDLKNIDKVRSEIGMCFQHFNLFPHLTILENCTLAPMLARQTPREEAEATAMRF 141 Query: 120 LKVVGLLDKANVYPATLSGGQQQRVAIARSLCTKKPYILFDEPTSALDPETIQEVLDVMK 179 L+ V + D+A YP LSGGQQQRVAIAR+LC K +LFDEPTSALDPE I+EVLD M Sbjct: 142 LEKVKIPDQAGKYPGQLSGGQQQRVAIARALCMKPRIMLFDEPTSALDPEMIKEVLDTMV 201 Query: 180 EISHQSNTTMVVVTHEMGFAKEVADRIIFMEDGAIVEENIPSEFFSNPKTERARLFLGKI 239 E++ + TM+ VTHEMGFA+ VA+R+IFM+ G IVEEN PS FF+NP+ ER + FL +I Sbjct: 202 ELA-EEGMTMLCVTHEMGFAQAVANRVIFMDQGRIVEENGPSAFFNNPQNERTQAFLSQI 260 Query: 240 L 240 + Sbjct: 261 I 261 Lambda K H 0.317 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 188 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 242 Length of database: 263 Length adjustment: 24 Effective length of query: 218 Effective length of database: 239 Effective search space: 52102 Effective search space used: 52102 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory