GapMind for catabolism of small carbon sources

 

Alignments for a candidate for malK_Bb in Marivita geojedonensis DPG-138

Align ABC-type maltose transport, ATP binding protein (characterized, see rationale)
to candidate WP_085638943.1 MGEO_RS13990 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC

Query= uniprot:Q6MNM2
         (347 letters)



>NCBI__GCF_002115805.1:WP_085638943.1
          Length = 350

 Score =  303 bits (775), Expect = 6e-87
 Identities = 165/355 (46%), Positives = 222/355 (62%), Gaps = 24/355 (6%)

Query: 1   MAKIQFSNIKKSFGSADVLKGIDLDIAPGEFLVLVGPSGCGKSTLLRTLAGLESADSGTI 60
           M+ +   ++ K +G   V+ G+DLD+  GEF V VGPSGCGKSTLLR +AGLE    GTI
Sbjct: 1   MSGVTLESVIKRYGQTQVIHGVDLDVQDGEFCVFVGPSGCGKSTLLRMVAGLEETTEGTI 60

Query: 61  SIDGKKINDIEPQNRDIAMVFQSYALYPHMTVAENMGFGLKLKNLAAAEITKRVNEISEL 120
            I G+ +  ++P +R ++MVFQ+YALYPHMTV ENMGFGLK+   AA +I ++V E S +
Sbjct: 61  RIGGRDVTRLDPSDRGVSMVFQTYALYPHMTVEENMGFGLKMTGHAAKDIKEKVAEASRI 120

Query: 121 LQIKHLLDRKPKELSGGQRQRVALGRALSRQTPVILFDEPLSNLDAHLRSQMRLEIKRLH 180
           L++   L RKPK LSGGQRQRVA+GRA+ R   V LFDEPLSNLDA LR +MR+EI RLH
Sbjct: 121 LKLDDYLKRKPKALSGGQRQRVAIGRAIVRGPEVFLFDEPLSNLDAELRVEMRVEIARLH 180

Query: 181 HNSKSTMIYVTHDQMEATTLGDRIAVLKDGVIEQIGTPSEIYHRPKNTFIATFIGSPEMN 240
               +TMIYVTHDQ+EA TL D+I VL+ G IEQ+G P E+Y  P N F+A FIGSP MN
Sbjct: 181 KEIGATMIYVTHDQVEAMTLADKIVVLRAGRIEQVGAPMELYRDPDNRFVAGFIGSPSMN 240

Query: 241 FLEGAV----------LEKIPWPEARKADQ--ILGIRPDAFALNQGPLGTQEVALGDFQI 288
           F+ G V          +  +    + +  Q  ++G+RP+   L  G            ++
Sbjct: 241 FIRGRVQGGEVVSDGLVHSVSKTASAQEGQEVLIGLRPEHLELRPG---------SSHRV 291

Query: 289 DISENLGGQQMLHGTLAGNNVRILVDSMDNF--SMKQTLPLKIDLTKAHLFDKKT 341
           D++E+LGG    H  +  +  +I+V+   +   S    + L +D     LFD KT
Sbjct: 292 DLTESLGGVSYAH-LIGPDGEKIIVEERGDHRSSDGDMVDLVVDPDHMFLFDAKT 345


Lambda     K      H
   0.318    0.136    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 307
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 347
Length of database: 350
Length adjustment: 29
Effective length of query: 318
Effective length of database: 321
Effective search space:   102078
Effective search space used:   102078
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory