Align High-affinity branched-chain amino acid transport ATP-binding protein BraF, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate WP_085640649.1 MGEO_RS17175 ABC transporter ATP-binding protein
Query= TCDB::P21629 (255 letters) >NCBI__GCF_002115805.1:WP_085640649.1 Length = 251 Score = 187 bits (476), Expect = 1e-52 Identities = 94/251 (37%), Positives = 155/251 (61%), Gaps = 3/251 (1%) Query: 5 ILEVSGLTMRFGGLLAVNGVNLKVEEKQVVSMIGPNGAGKTTVFNCLTGFYQPTGGLIRL 64 +LEV + RFGGL A+ VNL V E ++IGPNGAGK+T+ NCL G P G + Sbjct: 3 LLEVKDVNKRFGGLQALGNVNLSVAENTCHAIIGPNGAGKSTLLNCLVGKLIPDTGSVMF 62 Query: 65 DGEEIQGLPGHKIARKGVVRTFQNVRLFKEMTAVENLLVAQHRHLNTNFLAGLFKTPAFR 124 DG+ + G +I + G+ R FQ +F ++T +EN+L+ + +F ++ Sbjct: 63 DGQSVLGRTPFEINQMGISRVFQTPEIFGDLTVMENMLIPCFAKRDGSFALHGIRSVL-- 120 Query: 125 RSEREAMEYAAHWLEEVNLTEFANRSAGTLAYGQQRRLEIARCMMTRPRILMLDEPAAGL 184 SE E + A L++VN+ + N + +++ G +RRLE+A C++ P++L+LDEP AG+ Sbjct: 121 -SEGELRDQAEQMLDDVNMLDKRNMHSASMSRGDKRRLEMAMCLVQNPKLLLLDEPTAGM 179 Query: 185 NPKETDDLKALIAKLRSEHNVTVLLIEHDMKLVMSISDHIVVINQGAPLADGTPEQIRDN 244 +T++ L+ +++ + ++T+ +IEHDM +V S+++ I V+ QG PL + TPE I+ + Sbjct: 180 ARADTNNTIDLLKEIKEKRDITIAIIEHDMHVVFSLAERITVLAQGTPLVEDTPENIKGH 239 Query: 245 PDVIKAYLGEA 255 P V +AYLGEA Sbjct: 240 PKVKEAYLGEA 250 Lambda K H 0.320 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 168 Number of extensions: 4 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 255 Length of database: 251 Length adjustment: 24 Effective length of query: 231 Effective length of database: 227 Effective search space: 52437 Effective search space used: 52437 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory