Alignments for a candidate for SGLT1 in Alkalitalea saponilacus SC/BZ-SP2

GapMind for catabolism of small carbon sources

Alignments for a candidate for SGLT1 in Alkalitalea saponilacus SC/BZ-SP2

Align sodium/glucose cotransporter 1 (characterized)
to candidate WP_079555937.1 CDL62_RS06475 sodium/solute symporter

Query= CharProtDB::CH_091086
         (664 letters)



>NCBI__GCF_002201795.1:WP_079555937.1
          Length = 631

 Score =  241 bits (616), Expect = 6e-68
 Identities = 149/483 (30%), Positives = 253/483 (52%), Gaps = 21/483 (4%)

Query: 28  DISIIVIYFVVVMAVGLWAMFSTNRGTVGG---FFLAGRSMVWWPIGASLFASNIGSGHF 84
           D  I   Y  ++M +GLW +  T +G       +FLAG ++ WW IGASL A+NI + HF
Sbjct: 9   DYLIFGAYAALIMGIGLW-ISRTKKGEEKDSKDYFLAGGTLSWWAIGASLIAANISAEHF 67

Query: 85  VGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQRIQVY 144
           + ++G+G A G+ +  +EW A ++++++    +P  +   + TMP++ R+R+ G  +  +
Sbjct: 68  IAMSGSGYAIGLGMAAYEWIAAIVLILVAKYLLPQMLDKKIFTMPQFARERY-GSGVSFF 126

Query: 145 LSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTD 204
            S   LL+Y+F  +++  + GA+ +N  LG+ +   +  LL    +Y+I GG+ +V +TD
Sbjct: 127 FSFFWLLVYVFVNLTSVAWLGALAMNQILGVPILYGVPGLLIFAGVYSIYGGMKSVAWTD 186

Query: 205 TLQTVIMLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFH 264
            +Q   ++ G LI   FA   V G      +  + +   +    T          ++   
Sbjct: 187 IVQVFFLIFGGLITAWFALDAVAGDGKSAFEGFRVVLNQIRASETDTHFNMIIGDSEG-- 244

Query: 265 IFRDPLTGDLPWPGFIFGMSILT-LWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLM 323
             RD    +LP    IFG   LT + YW  +Q I+Q+ L+AKN+   K G +   YLK++
Sbjct: 245 -TRDSFL-NLPGLAVIFGAMWLTNIGYWGFNQFIIQKGLAAKNLKEAKRGLLFAAYLKIL 302

Query: 324 PMFIMVMPGMISRILY-------TEKIACVVPSECEKYCGTKVGCTNIAYPTLVVELMPN 376
              I+++PG+ + +++          +A +           +V  ++ AYP L+    P 
Sbjct: 303 IPIIVIIPGITAYVIHGMSVETADGSLAFINQMGDSTLLAGEVAISDDAYPWLLRNFAPV 362

Query: 377 GLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIY-AKVRKRASEKELMIAGRLFILVLIG 435
           G+RGL  + ++A+++SSL S+ NS ST+FT+DIY   + K ASE++L+  GR    V + 
Sbjct: 363 GIRGLAFAALVAAVISSLASLLNSTSTIFTLDIYKTLINKSASERQLVRVGRFAAFVALA 422

Query: 436 ISI-AWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLILGLL 494
           I+I A  P++      Q F YIQ  T ++ P + AVF + I WKR     A W  I  + 
Sbjct: 423 IAIVAARPLLGGLD--QAFQYIQEYTGFIYPGVVAVFGMGILWKRATNRAALWTTIATIP 480

Query: 495 IGI 497
            GI
Sbjct: 481 TGI 483


Lambda     K      H
   0.327    0.141    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 859
Number of extensions: 37
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 664
Length of database: 631
Length adjustment: 38
Effective length of query: 626
Effective length of database: 593
Effective search space:   371218
Effective search space used:   371218
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources