Alignments for a candidate for Echvi_1871 in Alkalitalea saponilacus SC/BZ-SP2

GapMind for catabolism of small carbon sources

Alignments for a candidate for Echvi_1871 in Alkalitalea saponilacus SC/BZ-SP2

Align SSS sodium solute transporter (characterized, see rationale)
to candidate WP_079555937.1 CDL62_RS06475 sodium/solute symporter

Query= uniprot:L0FZF3
         (547 letters)



>NCBI__GCF_002201795.1:WP_079555937.1
          Length = 631

 Score =  511 bits (1317), Expect = e-149
 Identities = 275/518 (53%), Positives = 357/518 (68%), Gaps = 25/518 (4%)

Query: 3   FNTLDLVVFVAYCLLIITMGIVVSREKKGHVKDSKDYFLASKALPWWAVGASLIASNISA 62
           F T+D ++F AY  LI+ +G+ +SR KKG  KDSKDYFLA   L WWA+GASLIA+NISA
Sbjct: 5   FTTIDYLIFGAYAALIMGIGLWISRTKKGEEKDSKDYFLAGGTLSWWAIGASLIAANISA 64

Query: 63  EQFIGMSGSGFALGLAISTYEWMAAATLLVVAIFFLPIYLKEGIYTMPQFLNRRYDGRVR 122
           E FI MSGSG+A+GL ++ YEW+AA  L++VA + LP  L + I+TMPQF   RY   V 
Sbjct: 65  EHFIAMSGSGYAIGLGMAAYEWIAAIVLILVAKYLLPQMLDKKIFTMPQFARERYGSGVS 124

Query: 123 TVMAIFWLLIYVFVNLTSVLYLGALSLETIMGVPLTYGIIGLALFAMVYSIYGGLKAVAW 182
              + FWLL+YVFVNLTSV +LGAL++  I+GVP+ YG+ GL +FA VYSIYGG+K+VAW
Sbjct: 125 FFFSFFWLLVYVFVNLTSVAWLGALAMNQILGVPILYGVPGLLIFAGVYSIYGGMKSVAW 184

Query: 183 TDVVQVVFLVAGGLATTYLALSLV-GDG-DVWEGIGIL-----RKAAPSHFSMIIEKGEM 235
           TD+VQV FL+ GGL T + AL  V GDG   +EG  ++          +HF+MII   E 
Sbjct: 185 TDIVQVFFLIFGGLITAWFALDAVAGDGKSAFEGFRVVLNQIRASETDTHFNMIIGDSE- 243

Query: 236 MIPDGSGGSRDAYLDLPGLSVLIGGMWIVNLNYWGCNQYITQRALAAKSLGEAQTGMVFA 295
                  G+RD++L+LPGL+V+ G MW+ N+ YWG NQ+I Q+ LAAK+L EA+ G++FA
Sbjct: 244 -------GTRDSFLNLPGLAVIFGAMWLTNIGYWGFNQFIIQKGLAAKNLKEAKRGLLFA 296

Query: 296 GFLKLLMPLIVVIPGIAAYVI---VQKGADAS--FIESMTDPVT---GLAKSDRAYPTLL 347
            +LK+L+P+IV+IPGI AYVI     + AD S  FI  M D       +A SD AYP LL
Sbjct: 297 AYLKILIPIIVIIPGITAYVIHGMSVETADGSLAFINQMGDSTLLAGEVAISDDAYPWLL 356

Query: 348 -HLLPPGLKGLAFAALTAAIVSSLASMANSTSTIFTIDIYKEFFNKNVSEGKQVTIGRIT 406
            +  P G++GLAFAAL AA++SSLAS+ NSTSTIFT+DIYK   NK+ SE + V +GR  
Sbjct: 357 RNFAPVGIRGLAFAALVAAVISSLASLLNSTSTIFTLDIYKTLINKSASERQLVRVGRFA 416

Query: 407 AVVAFIIAAIVA-PQLRQLDQAFQYIQEYTGFVSPGVFAIFIFGFFWKKTTSNAALTAAV 465
           A VA  IA + A P L  LDQAFQYIQEYTGF+ PGV A+F  G  WK+ T+ AAL   +
Sbjct: 417 AFVALAIAIVAARPLLGGLDQAFQYIQEYTGFIYPGVVAVFGMGILWKRATNRAALWTTI 476

Query: 466 LTIPLSAAFKVITPNLPFIDRMGVVFLVLSVLIIAISL 503
            TIP     K+  P+LP++ RMG VF++L  L I + L
Sbjct: 477 ATIPTGILMKLALPDLPWMIRMGYVFIILVGLSITLVL 514



 Score = 27.3 bits (59), Expect = 0.002
 Identities = 16/61 (26%), Positives = 31/61 (50%), Gaps = 3/61 (4%)

Query: 490 VFLVLSVL-IIAISLYEG--KGKDSKKAIEVDAELFSTSTKFKVGAVLICGILVALYSVF 546
           +F+  +++  +A+ +Y          KA + + ++  T   F   A+ I  I+VALY+ F
Sbjct: 571 IFMTTAMMTFLAVIMYTNAKSSLQDDKAYDFNPDIIKTDKVFFTMAMGIVVIIVALYAYF 630

Query: 547 W 547
           W
Sbjct: 631 W 631


Lambda     K      H
   0.326    0.140    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 989
Number of extensions: 42
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 547
Length of database: 631
Length adjustment: 37
Effective length of query: 510
Effective length of database: 594
Effective search space:   302940
Effective search space used:   302940
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources