Alignments for a candidate for D-LDH in Herbaspirillum aquaticum IEH 4430

GapMind for catabolism of small carbon sources

Alignments for a candidate for D-LDH in Herbaspirillum aquaticum IEH 4430

Align Probable D-lactate dehydrogenase, mitochondrial; DLD; Lactate dehydrogenase D; EC 1.1.2.4 (characterized)
to candidate WP_088755702.1 CEJ45_RS14000 FAD-binding protein

Query= SwissProt::F1QXM5
         (497 letters)



>NCBI__GCF_002213425.1:WP_088755702.1
          Length = 471

 Score =  473 bits (1216), Expect = e-138
 Identities = 237/447 (53%), Positives = 307/447 (68%), Gaps = 3/447 (0%)

Query: 46  EGVSVGSAVREQHGRDESVHRCRPPDVVVFPRSVEEVSALAKICHHYRLPIIPFGTGTGL 105
           E  S   A+RE HGRDES +    PD VVF  S EEV+A  K+C  Y  PIIP+G GT L
Sbjct: 28  ERCSTTLAMREHHGRDESSYDPMLPDAVVFAHSTEEVAAFVKLCSQYDTPIIPYGAGTSL 87

Query: 106 EGGVGALQGGVCFSLRKMEQVVDLHQEDFDVTVEPGVTRKSLNSYLRDTGLWFPVDPGAD 165
           EG V ALQGGV   L +M QV+ ++ ED   TV+ GVTRK LN  ++DTGL+FP+DPGAD
Sbjct: 88  EGHVLALQGGVTVDLSQMNQVLAVNAEDLTATVQAGVTRKQLNQEIKDTGLFFPIDPGAD 147

Query: 166 ASLCGMAATSASGTNAVRYGTMRENVLNLEVVLADGTILHTAGKGRRPRKTAAGYNLTNL 225
           ASL GMA+T ASGTNAVRYGTM+EN L L VV A G I+ T   G R +K++AGY+LT +
Sbjct: 148 ASLGGMASTRASGTNAVRYGTMKENTLTLTVVTAQGEIIKT---GTRAKKSSAGYDLTRV 204

Query: 226 FVGSEGTLGIITKATLRLYGVPESMVSAVCSFPSVQSAVDSTVQILQAGVPIARIEFLDD 285
           +VGSEGTLGIIT+ T+RLY  PE++ +A+CSFPSV  AV++ +Q +Q GVP+AR+E LD+
Sbjct: 205 YVGSEGTLGIITEVTVRLYPQPEAISAAICSFPSVADAVNTVIQTIQMGVPLARVELLDE 264

Query: 286 VMINACNRFNNLSYAVTPTLFLEFHGSSKSMEEQVSVTEEITRDNGGSDFAWAEDEETRS 345
             + A N  + L+  V P L  EFHGS   ++EQ  + ++I ++     F WA   E R+
Sbjct: 265 NGVRAINAHDKLNLPVNPLLLFEFHGSENGVKEQAELVQDIAKEFHALGFEWATRPEDRT 324

Query: 346 RLWKARHDAWYAAMALRPGCKAYSTDVCVPISRLPQIIVETKADLISNNITGPIAGHVGD 405
           RLW ARH+A++A + LRPG +A STD CVPISRL + ++ TKAD     +   I GHVGD
Sbjct: 325 RLWTARHNAYFALLQLRPGARAISTDCCVPISRLAECVLATKADCEEQGLIHAIIGHVGD 384

Query: 406 GNFHCLIVLDPNDTDEVQRVHSFTERLARRALAMDGTCTGEHGIGLGKRALLREEVGPLA 465
           GNFH  +++DPND  ++ R      R+  RAL MDGTCTGEHG+GL K   L +E G  A
Sbjct: 385 GNFHVQMMVDPNDPADIARAEGVNARMVARALGMDGTCTGEHGVGLHKMDFLIQEHGDGA 444

Query: 466 IEVMKGLKASLDPRNLMNPGKVLELTQ 492
           I VM+ +K +LDP+N+MNPGK+L   Q
Sbjct: 445 IAVMRAIKHALDPKNIMNPGKILRWEQ 471


Lambda     K      H
   0.319    0.135    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 566
Number of extensions: 19
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 497
Length of database: 471
Length adjustment: 34
Effective length of query: 463
Effective length of database: 437
Effective search space:   202331
Effective search space used:   202331
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources