GapMind for catabolism of small carbon sources

 

Protein WP_089136810.1 in Lactobacillus silagei IWT126

Annotation: NCBI__GCF_002217945.1:WP_089136810.1

Length: 873 amino acids

Source: GCF_002217945.1 in NCBI

Candidate for 22 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism ald-dh-CoA hi acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized) 57% 99% 967.2
2'-deoxyinosine catabolism ald-dh-CoA hi acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized) 57% 99% 967.2
2-deoxy-D-ribose catabolism ald-dh-CoA hi acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized) 57% 99% 967.2
ethanol catabolism ald-dh-CoA hi acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized) 57% 99% 967.2
L-threonine catabolism ald-dh-CoA hi acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized) 57% 99% 967.2
thymidine catabolism ald-dh-CoA hi acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized) 57% 99% 967.2
L-tryptophan catabolism ald-dh-CoA hi acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized) 57% 99% 967.2
ethanol catabolism etoh-dh-nad med alcohol dehydrogenase (EC 1.1.1.1); acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) (characterized) 53% 99% 919.8 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
4-hydroxybenzoate catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 45% 99% 714.5 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
2'-deoxyinosine catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 45% 99% 714.5 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
2-deoxy-D-ribose catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 45% 99% 714.5 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
ethanol catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 45% 99% 714.5 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
L-threonine catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 45% 99% 714.5 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
thymidine catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 45% 99% 714.5 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
L-tryptophan catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 45% 99% 714.5 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
L-arginine catabolism gabD lo succinate-semialdehyde dehydrogenase [NAD(P)+]; EC 1.2.1.16 (characterized) 39% 96% 301.6 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
L-citrulline catabolism gabD lo succinate-semialdehyde dehydrogenase [NAD(P)+]; EC 1.2.1.16 (characterized) 39% 96% 301.6 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
putrescine catabolism gabD lo succinate-semialdehyde dehydrogenase [NAD(P)+]; EC 1.2.1.16 (characterized) 39% 96% 301.6 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
L-fucose catabolism fucO lo Lactaldehyde reductase; Propanediol oxidoreductase; EC 1.1.1.77 (characterized) 33% 91% 190.3 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
L-rhamnose catabolism fucO lo Lactaldehyde reductase; Propanediol oxidoreductase; EC 1.1.1.77 (characterized) 33% 91% 190.3 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
L-threonine catabolism tdh lo L-threonine dehydrogenase (EC 1.1.1.103) (characterized) 35% 88% 184.5 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2
glycerol catabolism dhaD lo alcohol dehydrogenase (EC 1.1.1.1); long-chain-alcohol dehydrogenase (EC 1.1.1.192) (characterized) 37% 67% 153.7 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) 57% 967.2

Sequence Analysis Tools

View WP_089136810.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MLKEMNSKENNKQAKKPVSVNEHIDALVNKAHDALCGMDDFTQEKVDHIVHQMAIAGLDQ
HMRLAKLAVDETGRGVWEDKAIKNMFATEEIWHSIKNDKTVGIIEDDKERQLIKVAEPLG
ILAGVTPVTNPTSTTMFKSLIAVKTRNPIIFGFHPQAQKSSVAAAKVMRDAAIAAGAPEG
VIQWIEEPSLEATTALMNHPKVASVLATGGPGMVKAAYSTGKPALGVGPGNGPAYIEKTA
NIKRAVNDIVLSKTFDNGMICATENSVIIDADIYNDVKQEMKARKVFFIKKADQKALADA
MYDPKRGSVKGPIAGMAAVKIAKLAGIDVPEDTKVLAAELTTVGPDNMLSAEKLCPVISV
YKSADHKEGFKIANDLLHFGGLGHTAAIHTEDEDLATEYGIKMKASRVLVNTPSALGGIG
NLYNEMIPSLTLGTGSWGKNSVSHNVSSFDLLNIKTIAKRRNNMQWIKLPRVYFEKTSVR
YLGSMPGVKKAFIVADPAMISLGYVDKVLGELKRRPDGIEYQLFSVMESDPTTDTVEKGY
AQMKAFKPDTIIALGGGSPMDAAKCMWLFYEHPDASFFGAKQKFLDIRKRTYQFEKAKKA
QFVAIPTTSGTGSEVTPFAVITDSKTHVKYPLADYALTPDVAIVDSQFIESLPKKTISWA
GLDVLTHGIESFVSNMASDYTRPWSLQAIKLVFDNLTASYNGDQHAREEMHNAATIAGMA
FANAFLGVNHSIAHKLGGEFGLPHGLAIAITLPHVIRYNFKEPRKLTTWPKYEYFRADED
YAEIARYVGLTGKNNEELKEALVHKIIDLAHSVDVTLSLKANGVDKKHFDEKVDDLAELA
YEDQCTTANPKEPLIGELRQIMIDEYDGKGVEK

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory