pcaK, pobA, praA, xylF, mhpD, mhpE, ald-dh-CoA
Overview: 4-hydroxybenzoate catabolism in GapMind is based on aerobic oxidation to 3,4-hydroxybenzoate (protocatechuate), followed by meta, ortho, or para cleavage; or reduction to benzoyl-CoA (part of a MetaCyc pathway for phenol degradation, link)
Or see definitions of steps
| Step | Description | Best candidate | 2nd candidate |
|---|---|---|---|
| pcaK | 4-hydroxybenzoate transporter pcaK | ||
| pobA | 4-hydroxybenzoate 3-monooxygenase | ||
| praA | protocatechuate 2,3-dioxygenase | ||
| xylF | 2-hydroxymuconate semialdehyde hydrolase | ||
| mhpD | 2-hydroxypentadienoate hydratase | CES79_RS03435 | |
| mhpE | 4-hydroxy-2-oxovalerate aldolase | CES79_RS04090 | |
| ald-dh-CoA | acetaldehyde dehydrogenase, acylating | CES79_RS07645 | |
| Alternative steps: | |||
| ackA | acetate kinase | CES79_RS11275 | CES79_RS12115 |
| acs | acetyl-CoA synthetase, AMP-forming | ||
| adh | acetaldehyde dehydrogenase (not acylating) | CES79_RS07645 | CES79_RS01180 |
| atoB | acetyl-CoA C-acetyltransferase | CES79_RS08555 | |
| badH | 2-hydroxy-cyclohexanecarboxyl-CoA dehydrogenase | CES79_RS01690 | CES79_RS03935 |
| badI | 2-ketocyclohexanecarboxyl-CoA hydrolase | ||
| badK | cyclohex-1-ene-1-carboxyl-CoA hydratase | ||
| bamB | class II benzoyl-CoA reductase, BamB subunit | ||
| bamC | class II benzoyl-CoA reductase, BamC subunit | ||
| bamD | class II benzoyl-CoA reductase, BamD subunit | ||
| bamE | class II benzoyl-CoA reductase, BamE subunit | ||
| bamF | class II benzoyl-CoA reductase, BamF subunit | ||
| bamG | class II benzoyl-CoA reductase, BamG subunit | ||
| bamH | class II benzoyl-CoA reductase, BamH subunit | ||
| bamI | class II benzoyl-CoA reductase, BamI subunit | ||
| bcrA | ATP-dependent benzoyl-CoA reductase, alpha subunit | ||
| bcrB | ATP-dependent benzoyl-CoA reductase, beta subunit | ||
| bcrC | ATP-dependent benzoyl-CoA reductase, gamma subunit | ||
| bcrD | ATP-dependent benzoyl-CoA reductase, delta subunit | ||
| boxA | benzoyl-CoA epoxidase, subunit A | ||
| boxB | benzoyl-CoA epoxidase, subunit B | ||
| boxC | 2,3-epoxybenzoyl-CoA dihydrolase | ||
| boxD | 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase | ||
| catI | 3-oxoadipate CoA-transferase subunit A (CatI) | ||
| catJ | 3-oxoadipate CoA-transferase subunit B (CatJ) | ||
| Ch1CoA | cyclohex-1-ene-1-carbonyl-CoA dehydrogenase | ||
| dch | cyclohexa-1,5-diene-1-carboxyl-CoA hydratase | ||
| ech | (S)-3-hydroxybutanoyl-CoA hydro-lyase | ||
| fadB | (S)-3-hydroxybutanoyl-CoA dehydrogenase | CES79_RS00960 | CES79_RS10690 |
| fcbT1 | tripartite 4-hydroxybenzoate transporter, substrate-binding component FcbT1 | ||
| fcbT2 | tripartite 4-hydroxybenzoate transporter, small DctQ-like component FcbT2 | ||
| fcbT3 | tripartite 4-hydroxybenzoate transporter, large permease subunit FcbT3 | ||
| gcdH | glutaryl-CoA dehydrogenase | ||
| had | 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase | ||
| hcl | 4-hydroxybenzoyl-CoA ligase | ||
| hcrA | 4-hydroxybenzoyl-CoA reductase, alpha subunit | ||
| hcrB | 4-hydroxybenzoyl-CoA reductase, beta subunit | ||
| hcrC | 4-hydroxybenzoyl-CoA reductase, gamma subunit | ||
| ligA | protocatechuate 4,5-dioxygenase, alpha subunit | ||
| ligB | protocatechuate 4,5-dioxygenase, beta subunit | ||
| ligC | 2-hydroxy-4-carboxymuconate-6-semialdehyde dehydrogenase | ||
| ligI | 2-pyrone-4,6-dicarboxylate hydrolase | ||
| ligJ | 4-carboxy-2-hydroxymuconate hydratase | ||
| ligK | 4-oxalocitramalate aldolase | ||
| ligU | 4-oxalomesaconate tautomerase | ||
| oah | 6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase | ||
| paaF | 2,3-dehydroadipyl-CoA hydratase | ||
| paaH | 3-hydroxyadipyl-CoA dehydrogenase | CES79_RS00960 | CES79_RS10690 |
| paaJ2 | 3-oxoadipyl-CoA thiolase | CES79_RS08555 | |
| pcaB | 3-carboxymuconate cycloisomerase | ||
| pcaC | 4-carboxymuconolactone decarboxylase | CES79_RS01100 | |
| pcaD | 3-oxoadipate enol-lactone hydrolase | CES79_RS01100 | |
| pcaF | succinyl-CoA:acetyl-CoA C-succinyltransferase | CES79_RS08555 | |
| pcaG | protocatechuate 3,4-dioxygenase, beta subunit | ||
| pcaH | protocatechuate 3,4-dioxygenase, alpha subunit | ||
| pcaI | 3-oxoadipate CoA-transferase subunit A (PcaI) | ||
| pcaJ | 3-oxoadipate CoA-transferase subunit B (PcaJ) | ||
| pimB | 3-oxopimeloyl-CoA:CoA acetyltransferase | CES79_RS08555 | |
| pimC | pimeloyl-CoA dehydrogenase, small subunit | ||
| pimD | pimeloyl-CoA dehydrogenase, large subunit | ||
| pimF | 6-carboxyhex-2-enoyl-CoA hydratase | ||
| praB | 2-hydroxymuconate 6-semialdehyde dehydrogenase | CES79_RS01180 | |
| praC | 2-hydroxymuconate tautomerase | CES79_RS05575 | |
| praD | 2-oxohex-3-enedioate decarboxylase | ||
| pta | phosphate acetyltransferase | CES79_RS04940 | |
Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory