Align BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized)
to candidate WP_089136535.1 CES79_RS05345 methionine ABC transporter ATP-binding protein
Query= TCDB::Q9RQ06 (407 letters) >NCBI__GCF_002217945.1:WP_089136535.1 Length = 344 Score = 196 bits (498), Expect = 9e-55 Identities = 113/275 (41%), Positives = 166/275 (60%), Gaps = 5/275 (1%) Query: 24 MVEQGEPKNEILKKTGATVGVYDTNFEINEGEIFVIMGLSGSGKSTLLRLLNRLIEPTSG 83 M+E K G V D N I++GEI+ I+G SG+GKSTL R+LN L PTSG Sbjct: 1 MIEFKNVTKTFTTKDGDVTAVSDVNLNIDDGEIYGIVGYSGAGKSTLTRMLNGLETPTSG 60 Query: 84 KIFIDDQDVATLNKEDLLQVRRKSMSMVFQNFGLFPHRTILENTEYGLEVQNVPKEERRK 143 +I I+ + L+ +L + R+K + M+FQ+F L RT+++N + LEV V + +R+K Sbjct: 61 EIDINGDPITALSGPELRKKRQK-IGMIFQHFNLLWSRTVIQNIMFPLEVAGVSRSDRKK 119 Query: 144 RAEKALDNANLLDFKDQYPKQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREM 203 +A+ +D L + YP +LSGG +QRVG+ARALANDPEIL+ DEA SALDP E+ Sbjct: 120 KAQHLVDLVGLTGREHSYPSELSGGQKQRVGVARALANDPEILISDEATSALDPQTTDEV 179 Query: 204 QDELLELQAKFQKTIIFVSHDLNEALRIGDRIAIMKDGKIMQIGTGEEILTNPANDYVKT 263 D LLE+ K +I+ ++H+++ +I D +A+M DGK+++ GT + NP K Sbjct: 180 LDLLLEINKKMNLSIVLITHEMHAIRKICDHVAVMDDGKVVEQGTVFNVFRNPKKQITKR 239 Query: 264 FV-EDVDRAKVITAENIMIPALTTNIDVDGPSVAL 297 FV E+VD A +T N ++ L + DG V L Sbjct: 240 FVSEEVDPA--LTDTNSIVEDLIKSYP-DGTIVQL 271 Lambda K H 0.316 0.135 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 349 Number of extensions: 15 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 407 Length of database: 344 Length adjustment: 30 Effective length of query: 377 Effective length of database: 314 Effective search space: 118378 Effective search space used: 118378 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory