GapMind for catabolism of small carbon sources

 

Alignments for a candidate for braG in Lactobacillus silagei IWT126

Align High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate WP_089137089.1 CES79_RS10015 ABC transporter ATP-binding protein

Query= TCDB::P21630
         (233 letters)



>NCBI__GCF_002217945.1:WP_089137089.1
          Length = 259

 Score =  111 bits (278), Expect = 1e-29
 Identities = 79/254 (31%), Positives = 135/254 (53%), Gaps = 23/254 (9%)

Query: 1   MLSFDKVSTYYGKIQALHDVSVEVKKGEIVTLIGANGAGKSTLLMTLCGSPQAASGSIRY 60
           +L+ +++   +G + A+ DV++ +   E+V LIG NGAGK+TL   L G    +SGSI +
Sbjct: 4   LLNVEELVKNFGGLTAVSDVNMHLNDDELVALIGPNGAGKTTLFNLLTGVIGPSSGSITF 63

Query: 61  EGEE----LVGLPSSTIMRKSIAVVPEGRRVFSRLTVEENLAMG-------GFFTD---- 105
            G +    L G+ +  I    +A   +  R+F  L+V +N+ +        GFF      
Sbjct: 64  RGTDGTISLNGMKAYKIANLGMARTFQNIRLFKELSVLDNVLIAMTAHYKEGFFQSVFRL 123

Query: 106 ----KDDYQVQMD--KVLELFPRLKERYEQRAGTMSGGEQQMLAIGRALMSKPKLLLLDE 159
               K + Q++ +  ++L++F  L+   ++ A  +  G Q+ L I R L  KPK+L LDE
Sbjct: 124 PKFYKTESQIRKEAIELLKIFD-LQNDLDKLAKNLPYGIQRRLEIVRGLAMKPKVLFLDE 182

Query: 160 PSLGLAPIIIQQIFEIIEQLRRE-GVTVFLVEQNANQALKLADRAYVLENGRIVMHDTGA 218
           P+ G+ P     +  +I Q++R+  +TV L+E + +  + LA R YVL++G ++   T  
Sbjct: 183 PAAGMNPEETADLTRLIRQVQRDFHITVLLIEHDMSLVMNLASRIYVLDHGSLLAEGTPT 242

Query: 219 ALLTNPKVRDAYLG 232
            +  N  V  AYLG
Sbjct: 243 EIQQNKAVIKAYLG 256


Lambda     K      H
   0.318    0.136    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 129
Number of extensions: 5
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 233
Length of database: 259
Length adjustment: 24
Effective length of query: 209
Effective length of database: 235
Effective search space:    49115
Effective search space used:    49115
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory