Alignments for a candidate for pgmA in Ochrobactrum thiophenivorans DSM 7216

GapMind for catabolism of small carbon sources

Alignments for a candidate for pgmA in Ochrobactrum thiophenivorans DSM 7216

Align phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) (EC 5.4.2.2) (characterized)
to candidate WP_094506085.1 CEV31_RS05910 alpha-D-glucose phosphate-specific phosphoglucomutase

Query= BRENDA::Q9SCY0
         (623 letters)



>NCBI__GCF_002252445.1:WP_094506085.1
          Length = 543

 Score =  603 bits (1556), Expect = e-177
 Identities = 300/554 (54%), Positives = 403/554 (72%), Gaps = 14/554 (2%)

Query: 70  KSLPTKPIEGQKTGTSGLRKKVKVFMEDNYLANWIQALFNSLPLEDYKNATLVLGGDGRY 129
           K++ T P + Q+ GTSGLRKKV VF + NY  N+IQ++F+ L  + +   TLV+GGDGR+
Sbjct: 4   KTVATTPFQDQQPGTSGLRKKVPVFQQPNYAENFIQSVFDVL--DGFAGKTLVVGGDGRF 61

Query: 130 FNKEASQIIIKIAAGNGVGQILVGKEGILSTPAVSAVIRKRKANGGFIMSASHNPGGPEY 189
           +N+E  Q +IKIAA NG G+I+VG+ GILSTPA S +IR+ KA GG I+SASHNPGGP+ 
Sbjct: 62  YNREVIQKVIKIAAANGFGRIMVGQGGILSTPAASNMIRQYKAFGGLILSASHNPGGPKE 121

Query: 190 DWGIKFNYSSGQPAPETITDKIYGNTLSISEIKVAEIPDIDLSQVGVTKYGNFSVEVIDP 249
           D+GIK+N  +G PAPE IT+ I+  +  I + K+ + PD+D+ ++G  K G+  + V DP
Sbjct: 122 DFGIKYNIGNGGPAPEKITEAIFARSKVIDQYKITDAPDVDIDKIGTAKIGDTEIVVFDP 181

Query: 250 VSDYLELMEDVFDFDLIRGLLSRSDFGFMFDAMHAVTGAYAKPIFVDNLGAKPDSISNGV 309
           V DY  LME +FDFD IR ++ ++ FG  FDAMHAVTG YAK IF   LGA   S+ N V
Sbjct: 182 VEDYARLMETLFDFDAIRTMI-QNGFGLKFDAMHAVTGPYAKEIFERRLGAPEGSVMNFV 240

Query: 310 PLEDFGHGHPDPNLTYAKDLVDVMYRDNGPDFGAASDGDGDRNMVLGNKFFVTPSDSVAI 369
           PL DFG  HPDPNL YAK+L D++  D+ PDFGAASDGDGDRN+++G   FVTPSDS+A+
Sbjct: 241 PLPDFGGHHPDPNLVYAKELYDLLMSDHAPDFGAASDGDGDRNLIIGRGIFVTPSDSLAM 300

Query: 370 IAANAQEAIPYFRAGPKGLARSMPTSGALDRVAEKLKLPFFEVPTGWKFFGNLMDAGKLS 429
           +AANA  A P ++ G KG+ARSMPTS A DRVAEKL +  +E PTGWKFFGNL+D GK++
Sbjct: 301 LAANAHLA-PGYKDGIKGIARSMPTSAAADRVAEKLGIGMYETPTGWKFFGNLLDHGKVT 359

Query: 430 ICGEESFGTGSDHIREKDGIWAVLAWLSILAHRNKDTKPGDKLVSVADVVKEYWATYGRN 489
           ICGEES GTGSDH+REKDG+WAVL WL+ILA R +         SV  +V+E+WA +GRN
Sbjct: 360 ICGEESSGTGSDHVREKDGLWAVLLWLNILAVRKE---------SVKAIVEEHWARFGRN 410

Query: 490 FFSRYDYEECESEGANKMIEYLREILSKSKAGDVYGNYVLQFADDFSYTDPVDGSVASKQ 549
           +++R+DYE  +S+ A K++  LR  L+ S  G       ++ ADDF+Y DP+D SV+  Q
Sbjct: 411 YYTRHDYEAVDSDIAKKLVADLRGKLA-SLPGTSVNGLKIEKADDFAYHDPIDHSVSEHQ 469

Query: 550 GVRFVFTDGSRIIFRLSGTGSAGATVRIYIEQFEPDVSKHDVDAQIALKPLIDLALSVSK 609
           G+R  F  G+R++ RLSGTG++GAT+RIYIE++E D +KH++D Q  L  LID A  +++
Sbjct: 470 GIRIYFEGGARVVMRLSGTGTSGATIRIYIERYEADTAKHNLDTQQTLSTLIDAAEQIAE 529

Query: 610 LKDFTGREKPTVIT 623
           +K  +GR +P+VIT
Sbjct: 530 VKKRSGRSEPSVIT 543


Lambda     K      H
   0.317    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1030
Number of extensions: 70
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 623
Length of database: 543
Length adjustment: 36
Effective length of query: 587
Effective length of database: 507
Effective search space:   297609
Effective search space used:   297609
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources