Annotation: NCBI__GCF_002252475.1:WP_094578795.1
Length: 344 amino acids
Source: GCF_002252475.1 in NCBI
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| ethanol catabolism | etoh-dh-nad | hi | Alcohol dehydrogenase (EC 1.1.1.1) (characterized) | 77% | 100% | 542.7 | |||
| D-mannitol catabolism | mt1d | lo | mannitol dehydrogenase (EC 1.1.1.255) (characterized) | 32% | 88% | 173.7 | Alcohol dehydrogenase (EC 1.1.1.1) | 77% | 542.7 |
| L-threonine catabolism | tdh | lo | L-threonine 3-dehydrogenase (EC 1.1.1.103) (characterized) | 33% | 99% | 147.9 | Alcohol dehydrogenase (EC 1.1.1.1) | 77% | 542.7 |
| L-fucose catabolism | fdh | lo | D-threo-aldose 1-dehydrogenase (EC 1.1.1.122) (characterized) | 31% | 98% | 145.6 | Alcohol dehydrogenase (EC 1.1.1.1) | 77% | 542.7 |
View WP_094578795.1 at NCBI
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Find functional residues: SitesBLAST
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Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MAKTMKAAVVREFGKPLTIDEVPIPQPGDGQIQVAIQASGVCHTDLHAAEGDWPVKPNPP FIPGHEGVGFVSAVGKGVKHVKEGDRVGIPWLYTACGHCVHCLGGWETLCEEQLNTGYSV NGGFADYVVADPNFVGHLPKNIEFNEIAPILCAGVTVYKGLKVTDTKPGDWVVISGIGGL GHMAVQYAKAMGLNVAAVDIDDKKLDLARRLGATVTVNAKTHNDPAAYIKKETDGGAQGV LVTAVSPKAFEQAIGMAARGGTIALNGLPAGDFPLSIFNMVLNGVTVRGSIVGTRLDLQE SIDFAADGKVKATIRTDKIDNINAIFDEMRAGQIEGRVVMDLTQ
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory