Protein WP_099018712.1 in Marinicella litoralis KMM 3900
Annotation: NCBI__GCF_002591915.1:WP_099018712.1
Length: 294 amino acids
Source: GCF_002591915.1 in NCBI
Candidate for 33 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-arginine catabolism | artP | lo | Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) | 37% | 96% | 147.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-histidine catabolism | hisP | lo | Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) | 37% | 96% | 147.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-lysine catabolism | hisP | lo | Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) | 37% | 96% | 147.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-histidine catabolism | bgtA | lo | BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) | 38% | 90% | 144.4 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-citrulline catabolism | AO353_03040 | lo | ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized) | 36% | 98% | 142.9 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-glutamate catabolism | gltL | lo | GluA aka CGL1950, component of Glutamate porter (characterized) | 37% | 95% | 141.4 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-citrulline catabolism | PS417_17605 | lo | ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale) | 36% | 94% | 139.8 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| D-alanine catabolism | Pf6N2E2_5405 | lo | ABC transporter for D-Alanine, ATPase component (characterized) | 32% | 98% | 137.5 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-histidine catabolism | aapP | lo | ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, ATPase component (characterized) | 33% | 90% | 137.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-asparagine catabolism | aapP | lo | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 33% | 90% | 134.8 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-aspartate catabolism | aapP | lo | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 33% | 90% | 134.8 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-glutamate catabolism | aapP | lo | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 33% | 90% | 134.8 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-leucine catabolism | aapP | lo | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 33% | 90% | 134.8 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-proline catabolism | aapP | lo | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 33% | 90% | 134.8 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-asparagine catabolism | aatP | lo | ABC transporter for L-asparagine and L-glutamate, ATPase component (characterized) | 37% | 91% | 133.7 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-aspartate catabolism | aatP | lo | ABC transporter for L-asparagine and L-glutamate, ATPase component (characterized) | 37% | 91% | 133.7 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-proline catabolism | opuBA | lo | BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized) | 34% | 58% | 132.9 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-asparagine catabolism | bztD | lo | BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) | 33% | 90% | 132.5 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-aspartate catabolism | bztD | lo | BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) | 33% | 90% | 132.5 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| D-glucosamine (chitosamine) catabolism | AO353_21725 | lo | ABC transporter for D-glucosamine, ATPase component (characterized) | 35% | 99% | 128.6 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-histidine catabolism | BPHYT_RS24015 | lo | ABC transporter related (characterized, see rationale) | 33% | 95% | 128.6 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-histidine catabolism | hutV | lo | HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized) | 35% | 82% | 127.9 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-proline catabolism | hutV | lo | HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized) | 35% | 82% | 127.9 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-tryptophan catabolism | ecfA2 | lo | Energy-coupling factor transporter ATP-binding protein EcfA2; Short=ECF transporter A component EcfA2; EC 7.-.-.- (characterized, see rationale) | 34% | 77% | 115.2 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| D-cellobiose catabolism | gtsD | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 33% | 61% | 107.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| D-galactose catabolism | PfGW456L13_1897 | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 33% | 61% | 107.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| D-glucose catabolism | gtsD | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 33% | 61% | 107.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| lactose catabolism | gtsD | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 33% | 61% | 107.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| D-maltose catabolism | gtsD | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 33% | 61% | 107.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| sucrose catabolism | gtsD | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 33% | 61% | 107.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| trehalose catabolism | gtsD | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 33% | 61% | 107.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-phenylalanine catabolism | livF | lo | high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF (characterized) | 30% | 100% | 97.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
| L-valine catabolism | livF | lo | high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF (characterized) | 30% | 100% | 97.1 | phosphate ABC transporter, ATP-binding protein; EC 3.6.3.27 | 57% | 299.3 |
Sequence Analysis Tools
View WP_099018712.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MNKALLSKPSGLVEQALKVNKQTTTEDALNIDYTATVGQPNCQNAKFSTQNVNVYYANNQ
AIHDVSLDIGAHEVIALIGPSGCGKSTYLRCLNRMNDTIDICRVEGQILLDQENIYHQKQ
DPVLLRARVGMVFQKPNPFPKSIYDNVAYGPRIHGLTSNKAELDELVEASLIKAGLFQEV
KDRLQAPGTGLSGGQQQRLCIARTIAVQPEVILMDEPASALDPIATAKIEELINELKENF
TIVIVTHSMQQAARVSQRTAYFHMGRLIEVGDTNQIFTNPAHELTEKYITGRFG
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory