GapMind for catabolism of small carbon sources

 

Protein WP_099019330.1 in Marinicella litoralis KMM 3900

Annotation: NCBI__GCF_002591915.1:WP_099019330.1

Length: 590 amino acids

Source: GCF_002591915.1 in NCBI

Candidate for 9 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-lysine catabolism bcd lo butyryl-CoA dehydrogenase; EC 1.3.99.2 (characterized) 30% 90% 135.2 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5
L-isoleucine catabolism acdH lo 2-methyl-branched-chain-enoyl-CoA reductase (EC 1.3.8.5) (characterized) 33% 56% 102.1 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5
4-hydroxybenzoate catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 31% 60% 92 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5
L-arginine catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 31% 60% 92 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5
L-citrulline catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 31% 60% 92 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5
L-lysine catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 31% 60% 92 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5
phenylacetate catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 31% 60% 92 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5
L-phenylalanine catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 31% 60% 92 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5
L-proline catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 31% 60% 92 Acyl-CoA dehydrogenase (EC 1.3.8.7) 52% 625.5

Sequence Analysis Tools

View WP_099019330.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MANLPAPIKDIQFIADEVLDLTAHYKKLGVHDDIDAETFMTIVEEASKFLHENAGPLNRN
ADEQGCHHEGTSVTTPDGFKKLYKEFCESGWAALDGDPEYGGQGLSYWLQLILGELNTYY
CPGWSNYPGLTHGARELIAHFGSDVLKQKFLPKLTTGEWSGTMCLTEPHCGTDLGLVNTS
AKPNEDGSYDITGTKIFITSGEHDLTDNIIHLVLARLPDAPKGTKGISLFLVPKILLNAD
LELDQRNTLGAASIEHKMGLNGSATCVMNFDAAKGFLIGEGNDGLKIMFTMMNGARLNTG
IQGLSATQASYENALAYAKDRLQMRAATGAKYPNKPADPIIVHPDVRRMLLTMKAFAEGN
RAMAYFVGQQIEIIKHSKDQVEVKRADRLLAFLTPILKAFLTEVALEATDNGVQIFGGHG
YIREHGQEQWHRDAKIFTLYEGTTGIQALDLTGRKMLMMQKGQIGEMAELIQNFINETDT
PMNQELQNYLDMWQEMTEFITGQAQKNANEVGAASVDYLMMSGYVILAYFWAQLHNSAEN
NKGQLGADFYNGKKKTAQFYFAKILPRCHALKATIMTGADPLMNIKESEF

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory