Alignments for a candidate for puuC in Marinicella litoralis KMM 3900

GapMind for catabolism of small carbon sources

Alignments for a candidate for puuC in Marinicella litoralis KMM 3900

Align 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized)
to candidate WP_099017976.1 CCS90_RS02790 aldehyde dehydrogenase family protein

Query= metacyc::MONOMER-11560
         (497 letters)



>NCBI__GCF_002591915.1:WP_099017976.1
          Length = 787

 Score =  315 bits (808), Expect = 3e-90
 Identities = 193/475 (40%), Positives = 276/475 (58%), Gaps = 31/475 (6%)

Query: 23  FINGEYTDAVSGET-FECLSPVDGRFLAKVASCDLADANRAVENARATFNSGVWSQLAPA 81
           FING++    SG+  F   +P  G  LA+V+  D    N+AV+ A        W  L+  
Sbjct: 36  FINGDWH---SGKNHFASNNPASGERLAQVSQADKNTVNQAVKAAEKALPG--WQALSGH 90

Query: 82  KRKAKLIRFADLLRKNVEELALLETLDMGKPIGDSSSIDIPGAAQAIH----WTAEAIDK 137
            R   L   A L++KN    A+LETLD GKPI +S  ID+P A +  +    W A+ +D 
Sbjct: 91  ARSEYLYAIARLIQKNSRLFAVLETLDNGKPIRESRDIDVPLAIRHFYHHAGW-AQLLDT 149

Query: 138 VYDEVAPTPHDQLGLVTREPVGVVGAIVPWNFPLLMACWKLGPALATGNSVVLKPSEKSP 197
            + +              E +GVVG I+PWNFPLLM  WK+ PALATGN++V+KP+E + 
Sbjct: 150 EFPD-------------HEAIGVVGQIIPWNFPLLMLAWKIAPALATGNTIVIKPAEFTS 196

Query: 198 LTAIRIAQLAIEAGIPAGVLNVLPGYGHTVGKALALHMDVDTLVFTGSTKIAKQLMVYAG 257
           LTA+  A++  +AG+P GVLNV+ G G T G+ +  H D+  + FTGST + + +     
Sbjct: 197 LTALLFAEICQQAGLPKGVLNVVTGDGST-GQHIVEHPDIKKIAFTGSTAVGRWIRQATA 255

Query: 258 ESNMKRIWLEAGGKSPNIVFADAPDLQAAAEAAASAIAFNQGEVCTAGSRLLVERSIKDK 317
            S  K++ LE GGKS  IV ADA DL AA E    AI FNQG+VC AGSRLLV+ ++ ++
Sbjct: 256 GSG-KKLSLELGGKSAFIVCADA-DLDAAVEGVVDAIWFNQGQVCCAGSRLLVQEAVAER 313

Query: 318 FLPMVVEALKGWKPGNPLDPQTTVGALVDTQQMNTVLSYIEAGHKDGAKLLAGGKRTLE- 376
           F   ++  +   + G+PLD    +GA++D +Q   + S ++AG   GA L    + T E 
Sbjct: 314 FHKKLISRMHKLRIGDPLDKSIDMGAIIDPRQQQKIASLVQAGVDAGACL---NQSTAEL 370

Query: 377 ETGGTYVEPTIFDGVTNAMRIAQEEIFGPVLSVIAFDTAEEAVAIANDTPYGLAAGIWTS 436
            + G +  PT+   V+ + ++ Q+EIFGPVL  + F T  EAVA+AN++ YGLAA IW+ 
Sbjct: 371 PSSGCFYPPTLLTDVSPSDQVVQDEIFGPVLVSMTFRTPSEAVALANNSRYGLAASIWSE 430

Query: 437 DISKAHKTARAVRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKSLHALEKYTELK 491
           +I+ A   A  V+AG VW+N  +  D  A FGG K+SG GR+     L +Y  LK
Sbjct: 431 NINMAMHLAPLVQAGIVWINCTNMMDAAAGFGGVKESGFGREGGKEGLYEYLTLK 485



 Score = 83.2 bits (204), Expect = 3e-20
 Identities = 65/224 (29%), Positives = 106/224 (47%), Gaps = 10/224 (4%)

Query: 45  GRFLAKVASCDLADANRAVENARATFNSGVWSQLAPAKRKAKLIRFADLLRKNVEELAL- 103
           G+ +      +  D   AVE A AT +S  WS +   +R+  +   A+ L     E A  
Sbjct: 540 GQIITHAGLGNRKDVRNAVE-AAATSHS--WSHMNAYQRQQVMYFMAENLSYRKAEFATR 596

Query: 104 LETLDMGKPIGDSSSIDIPGAAQAIHWTAEAIDKVYDEVAPTPHDQLGLVTREPVGVVGA 163
           LE+    K   ++   ++  + Q + + A   DK   ++   P   + +   EP+GV+  
Sbjct: 597 LESFGHSKKQAET---EVESSIQRLSYYAAWCDKFDGQIHSVPMRGVVMAMHEPIGVIAI 653

Query: 164 IVPWNFPLLMACWKLGPALATGNSVVLKPSEKSPLTAIRIAQLAIEAGIPAGVLNVLPGY 223
             P   PLL     L PA+A GN VV+  SE  PL+A+   Q+   + +PAG +N++ G 
Sbjct: 654 QCPDEAPLLSFISLLAPAIAMGNRVVMTASEAFPLSALDFYQVLNTSDVPAGTVNIISGN 713

Query: 224 GHTVGKALALHMDVDTLVFTGSTKIAKQLMVYAGESNMKRIWLE 267
              + K LA HM+VD +   GS     Q + +   +N+KR W +
Sbjct: 714 RLELTKPLAGHMNVDAIWAFGSD---AQWVEHESATNLKRSWTQ 754


Lambda     K      H
   0.316    0.132    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 956
Number of extensions: 49
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 497
Length of database: 787
Length adjustment: 37
Effective length of query: 460
Effective length of database: 750
Effective search space:   345000
Effective search space used:   345000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources