Alignments for a candidate for adh in Marinobacter guineae M3B

GapMind for catabolism of small carbon sources

Alignments for a candidate for adh in Marinobacter guineae M3B

Align Aldehyde dehydrogenase (EC 1.2.1.3) (characterized)
to candidate WP_099618760.1 CLH62_RS14090 aldehyde dehydrogenase

Query= reanno::Marino:GFF3202
         (533 letters)



>NCBI__GCF_002744735.1:WP_099618760.1
          Length = 506

 Score =  926 bits (2393), Expect = 0.0
 Identities = 447/506 (88%), Positives = 474/506 (93%)

Query: 28  MIYAQPGKDGSVVSFKSRYENYIGGEWVAPVKGQYFENITPVTGNVICEIPRSSAEDIDL 87
           MIYAQPGKDGSVVSFK RYEN+IGGEWVAPVKGQYFENITPVTG+VICEIPRS AEDIDL
Sbjct: 1   MIYAQPGKDGSVVSFKPRYENFIGGEWVAPVKGQYFENITPVTGDVICEIPRSGAEDIDL 60

Query: 88  ALDAAHKAAPAWGKTSPTERSNILLKIADRIEANLEKLAVAETWDNGKAVRETLNADIPL 147
           ALDAAHKAAPAWGKTS TERSNILLKIADRIE N+E LAVAETWDNGKAVRET+NAD+PL
Sbjct: 61  ALDAAHKAAPAWGKTSTTERSNILLKIADRIEQNIEMLAVAETWDNGKAVRETINADLPL 120

Query: 148 AADHFRYFAGCLRAQEGHMGEIDHNTVAYHFHEPLGVVGQIIPWNFPILMAAWKLGPCLA 207
           A DHFRYFAGC+RAQE     ID+NTVAYH+HEPLGVVGQIIPWNFP+LMA WKL PCLA
Sbjct: 121 AVDHFRYFAGCIRAQEDTSSAIDNNTVAYHYHEPLGVVGQIIPWNFPLLMAVWKLAPCLA 180

Query: 208 AGNCTVLKPAEQTPASILVLMEIIGDLLPPGVLNIVNGYGIEAGQALATSKRIAKIAFTG 267
           AGNCTV+KPAEQTPASILVLME+IGDLLPPGV+NIVNGYGIEAGQALATSKRIAKIAFTG
Sbjct: 181 AGNCTVMKPAEQTPASILVLMELIGDLLPPGVINIVNGYGIEAGQALATSKRIAKIAFTG 240

Query: 268 STPVGSHILKCAAENIIPSTVELGGKSPNIYFSDVMKAEPEFIDKCVEGLVLAFFNQGEV 327
           STPVGSHILKCAAENIIPSTVELGGKSPNIYFSDVM+AEPEFIDKCVEGLVLAFFNQGEV
Sbjct: 241 STPVGSHILKCAAENIIPSTVELGGKSPNIYFSDVMQAEPEFIDKCVEGLVLAFFNQGEV 300

Query: 328 CTCPSRALVQEDMFEEFMQKVVQRTKSIKRGNPLDTDVQVGAQASKEQFDKIMSYLAIGK 387
           CTCPSRAL+QEDM++EFM KVV+RTK+IKRGNPLDTDVQVGAQASKEQFDKIMSYL IGK
Sbjct: 301 CTCPSRALIQEDMYDEFMAKVVERTKAIKRGNPLDTDVQVGAQASKEQFDKIMSYLEIGK 360

Query: 388 EEGAVVLTGGDREHLDEEFNNGFYIQPTLFKGDNKMRVFQEEIFGPVVGVTTFKTEEEAL 447
           +EGA VLTGG RE ++ ++NNGFY+QPTL KG N MRVFQEEIFGPVVGVTTFK EEEAL
Sbjct: 361 QEGAEVLTGGGREEMEGQYNNGFYVQPTLLKGRNDMRVFQEEIFGPVVGVTTFKDEEEAL 420

Query: 448 AIANDTEFGLGAGVWTRDTNLAYRMGRNIQAGRVWMNCYHAYPAHAAFGGYKKSGVGRET 507
           AIANDTEFGLGAG+WTRD N AYRMGR IQAGRVW NCYH YPAHAAFGGYKKSGVGRE 
Sbjct: 421 AIANDTEFGLGAGLWTRDINRAYRMGRAIQAGRVWTNCYHQYPAHAAFGGYKKSGVGREN 480

Query: 508 HKMALEHYQQTKCMLTSYDTNPLGFF 533
           HKMAL+HYQQTK +L SYD NPLGFF
Sbjct: 481 HKMALDHYQQTKNLLVSYDINPLGFF 506


Lambda     K      H
   0.319    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 951
Number of extensions: 26
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 533
Length of database: 506
Length adjustment: 35
Effective length of query: 498
Effective length of database: 471
Effective search space:   234558
Effective search space used:   234558
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources