Alignments for a candidate for lctP in Bacillus horneckiae 1P01SC

GapMind for catabolism of small carbon sources

Alignments for a candidate for lctP in Bacillus horneckiae 1P01SC

Align L-lactate permease (characterized, see rationale)
to candidate WP_066199278.1 CWS20_RS05260 L-lactate permease

Query= uniprot:L0GFN1
         (564 letters)



>NCBI__GCF_002835735.1:WP_066199278.1
          Length = 591

 Score =  553 bits (1425), Expect = e-162
 Identities = 284/555 (51%), Positives = 379/555 (68%), Gaps = 10/555 (1%)

Query: 1   MSNGLLALFAFTPILLAAIMLIGLRWPASRAMPLVFLFTAAIGLFVWDMSVNRIIASTLQ 60
           MS G+LA+ +  PI+  A+ L+GL+WPAS+AMP+ FL   A+ LFVW +    + A+++ 
Sbjct: 1   MSTGMLAILSLLPIIAVAVFLVGLKWPASKAMPIAFLVAVALALFVWKVPGVNVAAASVH 60

Query: 61  GLVITLGLLWIIFGAILLLNTLKHSGGITAIRAGFTTISPDRRIQAIIIAWLFGCFIEGA 120
           GLV+ + LL+IIFG+ILLLNTL+ SGG+  IR GFT I+ DRRIQ IIIAWLFG FIEGA
Sbjct: 61  GLVVAITLLYIIFGSILLLNTLQESGGMRTIRQGFTDITADRRIQVIIIAWLFGSFIEGA 120

Query: 121 SGFGTPAAIAAPLLVAVGFPAMAAVLLGMLVQSTPVSFGAVGTPIVVGINSGLDTATIGA 180
           SGFGTPAA+A PL+V +GFPAMAAV+ GM++QSTPVSFGAVGTPI+VG+ SGL       
Sbjct: 121 SGFGTPAAVAVPLMVGLGFPAMAAVVAGMVIQSTPVSFGAVGTPILVGVLSGLSAD---- 176

Query: 181 QLVAQGSSWNAYLQQITSSVAITHAIVGTVMPLVMVLMLTRFFGKEKSWKAGFEVLPFAI 240
              +   ++ A + QI   VAI H I GT++PL +V ++TRFFGK KS+  G ++  FA+
Sbjct: 177 --ASITDNFLALVSQIGGQVAILHMIAGTLVPLFVVALMTRFFGKNKSFSEGIKIWKFAL 234

Query: 241 FAGLAFTLPYAATGIFLGPEFPSLLGGLVGLAIVTTAARFKFLTPK-TTWDFADAKEWPA 299
           F+  A T+PY      LGPEFPS+ GGLVGLAIV  AA+  FL PK   W F +  +W  
Sbjct: 235 FSAFAMTIPYVIVANTLGPEFPSMTGGLVGLAIVIFAAKKGFLMPKDEVWQFEEKSKWNP 294

Query: 300 EWLGTIEMKLDEMAARPMSAFRAWLPYVLVGAILVISRVFP-QVTAALKSVSIAFANILG 358
           +W G IE++LD+   + MS F AWLPYVLVG  LV++R+    + A L+SV +   N+ G
Sbjct: 295 DWTGKIEIQLDK-KGKEMSMFTAWLPYVLVGLFLVLTRLNALPLKAWLQSVVLKIENLFG 353

Query: 359 ETGINAGIEPLYLPGGILVMVVLITFFLHGMRVSELKAAVKESSGVLLSAGFVLLFTVPM 418
            +GI    E LY PG + ++V ++TFF+HGMR      A K+S    L+A   L+FTVPM
Sbjct: 354 -SGIGTSFEILYSPGTVFILVSILTFFIHGMRSGAYVNAWKQSGKTTLAASVALVFTVPM 412

Query: 419 VRILINSGVNGAELASMPIVMARYVADSVGSIYPLLAPAVGALGAFLAGSNTVSNMMFSQ 478
           V++ IN+G   A    MPI +A   A   G  +P  A  +G +GAF+AGSNTVSNMMF+ 
Sbjct: 413 VQVFINTGGGAAGYNQMPIELANGAAALAGEFWPFFATFIGGIGAFIAGSNTVSNMMFAL 472

Query: 479 FQFGVAQSLGISGAMVVATQAVGAAAGNMVAIHNVVAASATVGLLGREGSTLRKTIWPTL 538
           FQ+ V   +G+    +VA QAVG AAGNM+ +HNVVAASA VGL+G+EG  +RKT++P +
Sbjct: 473 FQYDVGSQIGVDPTWIVALQAVGGAAGNMICVHNVVAASAVVGLVGKEGEVIRKTLFPFV 532

Query: 539 YYVLFTGVIGLIAIY 553
           YY LF G +G   ++
Sbjct: 533 YYSLFAGAVGYSIVW 547


Lambda     K      H
   0.326    0.140    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1048
Number of extensions: 49
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 564
Length of database: 591
Length adjustment: 36
Effective length of query: 528
Effective length of database: 555
Effective search space:   293040
Effective search space used:   293040
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources