Annotation: NCBI__GCF_002846395.1:WP_101442381.1
Length: 536 amino acids
Source: GCF_002846395.1 in NCBI
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| D-maltose catabolism | susB | med | α,α-trehalase / α-glucosidase (TTC0107) (EC 3.2.1.20|3.2.1.28) (characterized) | 52% | 100% | 572.8 | oligo-α-1,6-glucosidase (EC 3.2.1.10) | 53% | 566.6 |
| trehalose catabolism | treF | med | α,α-trehalase / α-glucosidase (TTC0107) (EC 3.2.1.20|3.2.1.28) (characterized) | 52% | 100% | 572.8 | alpha-glucosidase (EC 3.2.1.20) | 53% | 567.8 |
| sucrose catabolism | ams | med | Alpha-glucosidase; EC 3.2.1.- (characterized, see rationale) | 42% | 89% | 415.2 | α,α-trehalase / α-glucosidase (TTC0107) (EC 3.2.1.20|3.2.1.28) | 52% | 572.8 |
| trehalose catabolism | treC | lo | trehalose-6-P hydrolase (TreA;BlTreA;BLi00797;BL03069) (EC 3.2.1.93) (characterized) | 39% | 96% | 384.4 | α,α-trehalase / α-glucosidase (TTC0107) (EC 3.2.1.20|3.2.1.28) | 52% | 572.8 |
View WP_101442381.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MKDAEQKHLWWQEGVIYQIYPRSYQDSNGDGVGDLQGIIQRLDHIKSLGIKAIWISPIFS SPMADFGYDISDYCDIHPLFGTMDDFDELLQAVHERDMKLILDLVPNHTSDEHPWFKESR SSKDNPKRNWYIWQDANADGSEPNNWLSVFGGSAWEWDEKTGQYYYHAFLKEQPDLNWRN PEVQEAMLNVMRFWLDKGVDGFRVDVMWHMIKDDQLRDNPVNPNYKAHQATYEKLIPAYS TDQPEVHDIVHKMRRVLDSYDERMMIGEIYLPIHKLMTYYGTDKNGAHLPFNFLLLSIDW DATEISSNINQYEGALPDGGWPNWVLGNHDQPRITSRVGKAQARVAAMLLLTLRGTPTIY YGDEIGMRDVPIPPDEIQDPQGLNMPGLNLSRDPQRTPMQWDNSENAGFTQSKPWLRLPE DYRRVNVKAQLEDEFSIYALYKRLIKLRQDEPALHIGSYRPVVATGPLLAYLRYTGEQRF LILLNLSHKPCHFAPEHFSFEGTIVIDTLPEREGGKVRKHVSLSGDEGMIIRLNTA
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory