Alignments for a candidate for TM0028 in Pontibacter ramchanderi LP43

GapMind for catabolism of small carbon sources

Alignments for a candidate for TM0028 in Pontibacter ramchanderi LP43

Align TM0028, component of β-glucoside porter (Conners et al., 2005). Binds cellobiose, laminaribiose (Nanavati et al. 2006). Regulated by cellobiose-responsive repressor BglR (characterized)
to candidate WP_101444474.1 BD749_RS11520 ABC transporter ATP-binding protein

Query= TCDB::Q9WXN5
         (330 letters)



>NCBI__GCF_002846395.1:WP_101444474.1
          Length = 611

 Score =  139 bits (351), Expect = 1e-37
 Identities = 98/277 (35%), Positives = 144/277 (51%), Gaps = 22/277 (7%)

Query: 2   KEILLKAENVRAYYKLEKVS-------VKAVDGLSFEILEDEVIGVVGESGCGKTTLSNV 54
           K  LL+ ++++ Y+ ++K         VKAVDG+SFE+   E I +VGESG GKTTL   
Sbjct: 326 KPPLLQVQDLQVYFPIKKGIFSRTTGYVKAVDGVSFEVKHGETIALVGESGSGKTTLGRA 385

Query: 55  IF--MNMVKPLTLVDGKIFLRVNGEFVELSSMTRDEVKRKFWGKEITIIPQAAMN-ALMP 111
           I   +       L +GK    +N + +      R   +  F     ++ P   +  A++ 
Sbjct: 386 ILRLVESTAGSVLFEGKDIASMNTKTLR---QNRRHFQMIFQDPYTSLNPMHTVGEAILE 442

Query: 112 TIRMEKYVRHLAESHGIDEEELLDKARRRFEEVGLDPLWIKRYPFELSGGMRQRAVIAIA 171
            +R+ K      E  G    E+L+      E+VGL P   +RYP   SGG RQR  IA A
Sbjct: 443 PMRVHKLYGSDKERRG----EMLEL----IEKVGLSPEHAQRYPQAFSGGQRQRIAIARA 494

Query: 172 TILNPSLLIADEPTSALDVVNQKVLLKVLMQMKRQGIVKSIIFITHDIATVRQIADRMII 231
             L P LLI DE  SALDV  Q  +L +L ++KR     + +FITHD+A  + +ADR+++
Sbjct: 495 LALQPKLLICDESVSALDVSVQAQVLNLLNELKRD-FNMTYLFITHDLAVAKHMADRILV 553

Query: 232 MYAGKIVEFAPVESLLEKPLHPYTQGLFNSVLTPEPE 268
           M+ G+IVE      L + P H YT+ L  ++   EPE
Sbjct: 554 MHEGRIVEQGIPVQLFQNPQHDYTRSLIQAIPGGEPE 590



 Score =  130 bits (328), Expect = 7e-35
 Identities = 86/259 (33%), Positives = 149/259 (57%), Gaps = 9/259 (3%)

Query: 5   LLKAENVRAYYKLEKVSVKAVDGLSFEILEDEVIGVVGESGCGKTTLSNVIFMNMVKPLT 64
           +L+  ++   +   +  V+AVD +SF +   E + +VGESG GKT ++ +  M ++    
Sbjct: 7   ILQVTDLETTFATRQGIVRAVDKVSFALYPGEAVAIVGESGSGKTVMA-LSLMQLLDTNA 65

Query: 65  LVDGK-IFLRVNGEFVELSSMTRDEVKRKFWGKEITIIPQAAMNALMPTIRM-EKYVRHL 122
            V GK +F       V+L  +   ++++   G E+ +I Q  M++L P     ++ V  L
Sbjct: 66  QVGGKAVFQSERLGAVDLLQLQEKQLQQ-LRGNEMGMIFQDPMSSLNPVYTCGQQVVEVL 124

Query: 123 AESHGIDEEELLDKARRRFEEVGLD-PLWI-KRYPFELSGGMRQRAVIAIATILNPSLLI 180
                I ++E  ++  + FE+  L  P  I   YP ++SGG +QR +IA+A    P++LI
Sbjct: 125 LWHRKISKKEARERVLQLFEQAKLPRPEQIYDSYPHQISGGQKQRVIIAMAMACEPAILI 184

Query: 181 ADEPTSALDVVNQKVLLKVLMQMK-RQGIVKSIIFITHDIATVRQIADRMIIMYAGKIVE 239
           ADE T+ALDV  Q  +L ++ +++ +Q +  +++FI+HD+  V +IADR+++MY G+IVE
Sbjct: 185 ADESTTALDVTVQARMLSLIDELRVKQNM--AVLFISHDLGVVAEIADRVLVMYKGRIVE 242

Query: 240 FAPVESLLEKPLHPYTQGL 258
              V  +   P HPYT+GL
Sbjct: 243 QGKVLDIFTNPQHPYTKGL 261


Lambda     K      H
   0.321    0.138    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 476
Number of extensions: 28
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 330
Length of database: 611
Length adjustment: 33
Effective length of query: 297
Effective length of database: 578
Effective search space:   171666
Effective search space used:   171666
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources