Alignments for a candidate for fadB in Pontibacter ramchanderi LP43

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadB in Pontibacter ramchanderi LP43

Align Probable 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 (characterized)
to candidate WP_101447209.1 BD749_RS18290 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein

Query= SwissProt::O32178
         (789 letters)



>NCBI__GCF_002846395.1:WP_101447209.1
          Length = 800

 Score =  758 bits (1958), Expect = 0.0
 Identities = 396/801 (49%), Positives = 539/801 (67%), Gaps = 13/801 (1%)

Query: 1   MHKHIRKAAVLGSGVMGSGIAAHLANIGIPVLLLDIVPNDLTKEEEKKGLTKDSSEVRSR 60
           M + I+K AVLGSGVMGS IA H ANIG+ VLLLDIVP +LT +EEKKGLT +S  VR+R
Sbjct: 1   MKRIIKKVAVLGSGVMGSRIACHFANIGVQVLLLDIVPRELTPDEEKKGLTLESKHVRNR 60

Query: 61  LSRQAMKKLLKQKPAPLTSAKNTSYITPGNLEDDAEKLKEADWIIEVVVENLEVKKKIFA 120
           +   A++  +   P+PL    +   I  GN ED+ +++  ADW IEVVVENL++KK +F 
Sbjct: 61  IVNSALQAAINSNPSPLYRKSDARLIQTGNFEDNMKEIATADWTIEVVVENLKIKKTVFD 120

Query: 121 LVDEHRKTGSIVSSNTSGISVQEMAEGRSDDFKAHFLGTHFFNPARYLKLLEIIPIKETD 180
            V++HRK G++++SNTSGI +  M EGRSDDFK HF GTHFFNP RYLKLLEIIP  +TD
Sbjct: 121 QVEQHRKPGTLITSNTSGIPIHMMLEGRSDDFKKHFCGTHFFNPPRYLKLLEIIPTPDTD 180

Query: 181 PDILKFMTAFGENVLGKGVVTAKDTPNFIANRIGTYGLLVTVQEMLKGGYQVGEVDSITG 240
            D++ F+  +G+  LGK  V AKDTP FIANR+G Y ++  +Q M K G  V EVD I+G
Sbjct: 181 QDVVDFLMHYGDLYLGKTTVLAKDTPAFIANRVGIYAIMQGLQVMNKLGLNVDEVDRISG 240

Query: 241 PLIGRPKSATFRTLDVVGLDTFAHVARNVYDKADGDE-KEVFRIPSFMNDMLEKGWIGSK 299
           P+IGRPKSATFRTLDVVGLDT A+VA  +Y   + DE +E+F++P ++  M+E  W+G K
Sbjct: 241 PMIGRPKSATFRTLDVVGLDTLANVANGLYQTGEQDESRELFKLPDYLQKMVENKWLGDK 300

Query: 300 AGQGFYKK----EGKT-IYELDPVTLTYGERTKMKSPALEAAKQAKGTKAKMKALIYSDD 354
            GQGFYKK    +GKT I  LD  T+ YG + + K  +LE  K  +  K ++KA    +D
Sbjct: 301 TGQGFYKKTKDAKGKTEILTLDLNTMEYGPKQRAKFQSLEVLKPIEDLKKRIKAFSKQED 360

Query: 355 RAGRLLWNITSQTLLYSAELLGEIADDIHAIDQAMKWGFGWELGPFEMWDAIGLKQSAEK 414
           +A +           Y +  + EI+D+++ ID AM+ GFGWELGPFE WDAIG ++  ++
Sbjct: 361 KAAKFFNESLYGLFQYVSNRIPEISDELYRIDDAMRAGFGWELGPFEYWDAIGAREGVQR 420

Query: 415 LEQLGADMPGWIKEMLDKGNETFYIKENGTVFYYD--RGEYRAVKENKKRIHLQALKETK 472
           + + G     W++EML+ G E+FY+ ENG   YYD    EY+A+   +  I L+ L+  K
Sbjct: 421 MIENGYQPARWVEEMLEHGKESFYVVENGNRRYYDINSKEYKAIPGAENFIILENLRGNK 480

Query: 473 GVIAKNSGASLIDLGDDVALLEFHSKSNAIGLDIIQMIHKGLEETERNYKGLVIGNQGKN 532
            +I KNSGAS+IDLGD +  +EFH+K N +G D+I  ++KG+E  E++++G+V+GNQG N
Sbjct: 481 -LIWKNSGASIIDLGDGILNVEFHTKMNTMGGDVIMALNKGIELAEKDFRGMVVGNQGTN 539

Query: 533 FCVGANLAMILMEVQDDNFLEVDFVIRRFQETMMKIKYSAKPVVAAPFGMTLGGGTEACL 592
           F  GAN+ +I M   D ++ E++ +IR+FQ TMM+++YSA PVV AP G+TLGGG E  L
Sbjct: 540 FSAGANVGLIYMYALDQDYDELNMIIRQFQNTMMRMRYSAIPVVGAPHGLTLGGGCELNL 599

Query: 593 PAARIQAASEAYMGLVESGVGLIPGGGGNKELYI---NHLRRGHDPMNAAMKTFETIAMA 649
               IQAA+E YMGLVE GVGLIPGGGG KE+ +   +    G   +N     F  I MA
Sbjct: 600 HCDHIQAAAETYMGLVEFGVGLIPGGGGTKEMTLRAADMYADGDIELNDLKNVFLNIGMA 659

Query: 650 KVSASAQEAREMNILKETDQISVNQDHLLYDAKQLAASLYDTGWRPPV-KEKVKVPGETG 708
           KVS SA+EA ++  ++++D ISVN +  + DAK  A  L + G+  P+ +  +KV G   
Sbjct: 660 KVSTSAKEAIDLGYIRKSDGISVNSNRQIADAKAQAIRLAEAGYTKPIQRTNIKVQGRGA 719

Query: 709 YAALLLGAEQMKLSGYISEHDFKIAKKLAYVIAGGKVPFGTEVDEEYLLEIEREAFLSLS 768
               L GA  M    Y+SEHD KI++KLAYV+ GG +   TEV E+YLL++EREAFLSL+
Sbjct: 720 LGMFLTGANAMFTGRYMSEHDLKISQKLAYVMCGGDLSAPTEVSEQYLLDLEREAFLSLT 779

Query: 769 GEAKSQARMQHMLVKGKPLRN 789
           GE K+  R+Q +L  GKPLRN
Sbjct: 780 GERKTLERIQSILTTGKPLRN 800


Lambda     K      H
   0.316    0.134    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1474
Number of extensions: 69
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 789
Length of database: 800
Length adjustment: 41
Effective length of query: 748
Effective length of database: 759
Effective search space:   567732
Effective search space used:   567732
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources