Alignments for a candidate for malK_Ss in Pontibacter ramchanderi LP43

GapMind for catabolism of small carbon sources

Alignments for a candidate for malK_Ss in Pontibacter ramchanderi LP43

Align MalK, component of Maltose and maltooligosaccharide porter (characterized)
to candidate WP_101444474.1 BD749_RS11520 ABC transporter ATP-binding protein

Query= TCDB::Q97UG5
         (617 letters)



>NCBI__GCF_002846395.1:WP_101444474.1
          Length = 611

 Score =  353 bits (906), Expect = e-101
 Identities = 202/617 (32%), Positives = 357/617 (57%), Gaps = 51/617 (8%)

Query: 8   LLKVNELTAGYFNQDGFVIGVTNVNFEVYPNEIFAIAGESGCGKSTLAMAIYGLLKYPGV 67
           +L+V +L   +  + G V  V  V+F +YP E  AI GESG GK+ +A+++  LL     
Sbjct: 7   ILQVTDLETTFATRQGIVRAVDKVSFALYPGEAVAIVGESGSGKTVMALSLMQLLDTNAQ 66

Query: 68  VLRGHVYLKDK----DILSITQEELRKLRMKEFVYVPQFAMDALDPVAKIGDQMMRAAVS 123
           V    V+  ++    D+L + +++L++LR  E   + Q  M +L+PV   G Q++   + 
Sbjct: 67  VGGKAVFQSERLGAVDLLQLQEKQLQQLRGNEMGMIFQDPMSSLNPVYTCGQQVVEVLLW 126

Query: 124 HG-VNVEEARKLIKEKLELVDLPY--NVVNMYPHELSGGMRQRVVIATSILLNPSLIILD 180
           H  ++ +EAR+ + +  E   LP    + + YPH++SGG +QRV+IA ++   P+++I D
Sbjct: 127 HRKISKKEARERVLQLFEQAKLPRPEQIYDSYPHQISGGQKQRVIIAMAMACEPAILIAD 186

Query: 181 EPTTGLDVIVQYEILKDLKRIQRQLGVSLVIISHDISMLLMISDRVGIMYAGEIVEIGSK 240
           E TT LDV VQ  +L  +  ++ +  ++++ ISHD+ ++  I+DRV +MY G IVE G  
Sbjct: 187 ESTTALDVTVQARMLSLIDELRVKQNMAVLFISHDLGVVAEIADRVLVMYKGRIVEQGKV 246

Query: 241 EEIIKRPSHPYTYLLISSLPSLVKRREKLLSIPGNPPLMLSKVPNSCRFYD---RCPFKM 297
            +I   P HPYT  L++  P+L  + +             +K+P    F +   +   + 
Sbjct: 247 LDIFTNPQHPYTKGLLACRPTLSTKSQ-------------AKLPTVADFMEEDGQGIIRE 293

Query: 298 EKCSTLNPALGDIMDGHKARCFLQKGGYVDLSTLPIPLEYYAEEKAETDLSESNQHEVVM 357
           +K     P+L D+++ +                    +   ++ K + D   ++Q   ++
Sbjct: 294 KKPQVYEPSLADVVNAY--------------------VGTVSDIKQQRD---NSQKPPLL 330

Query: 358 KILNLSKIYYIRKNLI--LSEPINAVNDVSFELKKGTITALVGGSGHGKSTIAKILAGMI 415
           ++ +L   + I+K +    +  + AV+ VSFE+K G   ALVG SG GK+T+ + +  ++
Sbjct: 331 QVQDLQVYFPIKKGIFSRTTGYVKAVDGVSFEVKHGETIALVGESGSGKTTLGRAILRLV 390

Query: 416 QQTSGKIILLGKDVSEYGVRNSMWYKENVQMIFQDPYSSLDPRHTVRWHVERPLLIHKKV 475
           + T+G ++  GKD++    +     + + QMIFQDPY+SL+P HTV   +  P+ +HK  
Sbjct: 391 ESTAGSVLFEGKDIASMNTKTLRQNRRHFQMIFQDPYTSLNPMHTVGEAILEPMRVHKLY 450

Query: 476 SNKDQLLPKIIEVLKNVGLKPPEKYLYKYPHELSGGERQRVAIARATAVEPKVLVADEPV 535
            +  +   +++E+++ VGL P  ++  +YP   SGG+RQR+AIARA A++PK+L+ DE V
Sbjct: 451 GSDKERRGEMLELIEKVGLSP--EHAQRYPQAFSGGQRQRIAIARALALQPKLLICDESV 508

Query: 536 SMLDASLRAGILNLIKKFKKN-GISILYITHDIATVNYIADEIMVIYKGRIVEKGNTYEV 594
           S LD S++A +LNL+ + K++  ++ L+ITHD+A   ++AD I+V+++GRIVE+G   ++
Sbjct: 509 SALDVSVQAQVLNLLNELKRDFNMTYLFITHDLAVAKHMADRILVMHEGRIVEQGIPVQL 568

Query: 595 ISNPSHEYTKRLIEAVP 611
             NP H+YT+ LI+A+P
Sbjct: 569 FQNPQHDYTRSLIQAIP 585


Lambda     K      H
   0.319    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 892
Number of extensions: 38
Number of successful extensions: 12
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 617
Length of database: 611
Length adjustment: 37
Effective length of query: 580
Effective length of database: 574
Effective search space:   332920
Effective search space used:   332920
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources