GapMind for catabolism of small carbon sources

 

Protein WP_084199337.1 in Halioglobus japonicus S1-36

Annotation: NCBI__GCF_002869505.1:WP_084199337.1

Length: 1803 amino acids

Source: GCF_002869505.1 in NCBI

Candidate for 14 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine catabolism hpcD hi propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) (characterized) 53% 100% 1873.2
propionate catabolism hpcD hi propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) (characterized) 53% 100% 1873.2
L-threonine catabolism hpcD hi propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) (characterized) 53% 100% 1873.2
L-valine catabolism hpcD hi propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) (characterized) 53% 100% 1873.2
4-hydroxybenzoate catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
L-lactate catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
acetate catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
2'-deoxyinosine catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
2-deoxy-D-ribose catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
ethanol catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
L-threonine catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
thymidine catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
L-tryptophan catabolism acs lo Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 32% 81% 258.5 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2
propionate catabolism prpE lo Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 (characterized) 32% 67% 231.9 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) 53% 1873.2

Sequence Analysis Tools

View WP_084199337.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MQNIYSPATPEDVRENPIQSLEDWQRMRKACEEDLGAFHADIAAREIHWFNPQTNSWLTR
VQQDHWQGWHAHTAKPADALAWQPWQQALDESSAPFYRWFSGAMTNAAFNELDRHVLAGH
GDEIAFYYEGDRWDPALNAGKGGPVQHNAISRRELLLRSVIAAQALLELGLVKGDRIAIN
MPNILEQIVWTEAAKRIGVIYTPVFGGFSDKTLADRIENAGASVVITADGASRNAELVPF
KEAYCDPALDLYISLKSALAAIADASANAAIGQRICRRVAEGLAGEISVTRADVMREAGQ
VLAELDIEPEAVASLRGDLAETLSRTRSGVQKVIVVRHADLPEISWHEGRDLWAEDILIQ
AEATILSNAGLQKRSELMALGDTELTQALWRSVKPVPVSADFPLFIIYTSGSTGKPKGVV
HVHGGYTAGVSYSTKISFDARAGEDIIWVIADPGWITGQSYLISAALTNRLTSVVTEGPP
TFPNAGRFASIIERYGVTIFKAGVTFLKGIISNPQNRLDVERYSRSSVRVATFCAEPTSP
SVQRFAMDLVTPQYINSYWATEHGGIVWTHPYGNAEMPLRADAQTYPLPWIFGDIWIGNS
EPDETGRVTYSQARPDEKGEIVITRPYPYLARTIWGDPDNVNSDEWMGDIERFKATYFGR
YRSDSGEPEYTYVQGDFARRYTNGSYSLHGRSDDVINVSGHRMGTEEIEGAILKDRQLNP
ESKVGNCIVIGAPHQDKGLTPLAFILPVARQSLSSDDERHLAELVQTEKGSLAVPSAFLC
VDAFPETRSGKYLRRFLKAIVLDQELGDTSTLRNPECLPKIRRVVEQWRRQEDRANQQAI
IESSRTLRVEYVPVTTSGERTQCIATITLDNPPVNALSERVLDELETILTHIRRRDDIGA
AVITGRGGKTFVAGADVRQLLDEVHDKSTALSMPSKAHSVTSLIESMDKPVIAAIDGAAL
GGGCELAMACHWRVGNQYTQLGQPEINLFLPPGYGGTQRLTRIMADAAGAQQQSEGLTRA
LAMMLSGRAIDGMTGERYGLIDHLCRGNESVLAAAQAAAREACLDGKGVAVTAMRARHEA
VKRWQTPQEFPVECLRDDYVRKCLDASELAGRGPVAKAIVHLVRHGWKVGFEAGLAQEAE
TFSRYVLDERHGGKRGIKLFLDKHSPALPARPRVSTKLKDTLIRQGDMLPMGAPFFAGIT
PIPSYQMAQAVQRDPKTGVALHGDPADAEHEVIKPVPSPGPNQALLYMLTSEVNYNDIWA
VTGIPISLFDEHDEDLHVTGSGGVGIVASLGEALQQEGRITLGQLVTVYSGVSDLLDPTA
ADDPMYTEFHIQGYQSPDGSHQQFMLADGPQLIAPASGLSIEAMGSYQLPAGTAYRSLFG
ALGTHQCDRLFVEGAASGTGAWTVELGRAIGATVTGLVSSENRVSYLEGLGAAGINRRDA
DLISCFTRIPRDASQWHDWERQGTRFLEQVREANQGDLCTQAVSHAGETAFPRSFQALEA
GGAITFFGASSGYYMTFMGKPGSEPPATMLRRAQALPGQACVILYGKNNKVRDERAMAAL
EAARAYSLRIAVLTDTDAQRDFVLSLGYGDAVMGAVSLEEIRRREPLFEWPETMPELPAP
ESSEFKEAVRLMNEHTLKPVGNALGKLLRSSDNPRGQPDIIMERAHNDSLFASVMLVRPH
TGRVVYCDDMAARRYSFYAPQVWMRQRSIIMPTAKIIGTHLCNAAELIHLNRMISSGAVS
VPEPYLAQWGDTPMMHQAMWENRLPEVTDGAVKAVVNHALPCKNLKTYDELLAAWSMAEQ
IES

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory