Alignments for a candidate for mcm-large in Halioglobus japonicus S1-36

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcm-large in Halioglobus japonicus S1-36

Align methylmalonyl-CoA mutase (EC 5.4.99.2) (characterized)
to candidate WP_084199764.1 C0029_RS11185 methylmalonyl-CoA mutase

Query= BRENDA::P11653
         (728 letters)



>NCBI__GCF_002869505.1:WP_084199764.1
          Length = 719

 Score =  863 bits (2229), Expect = 0.0
 Identities = 438/694 (63%), Positives = 530/694 (76%), Gaps = 10/694 (1%)

Query: 27  ELAAKAGTGE-----AWETAEQIPVGTLFNEDVYKDMDWLDTYAGIPPFVHGPYATMYAF 81
           ELA K   G+      W+T E I V  L+     +D+++ DT  G+ P++ GP ATMYA 
Sbjct: 19  ELATKQLRGKPLDDLTWQTPEDIDVKPLYTAADTEDLEYADTMPGMSPYIRGPQATMYAG 78

Query: 82  RPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLPTHRGYDSDNPRVAGDVGMAGV 141
           RPWTIRQYAGFSTA+ESNAFYR+ LAAG +G+SVAFDL THRGYDSD+PRV+GDVG AGV
Sbjct: 79  RPWTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHRGYDSDHPRVSGDVGKAGV 138

Query: 142 AIDSIYDMRELFAGIPLDQMSVSMTMNGAVLPILALYVVTAEEQGVKPEQLAGTIQNDIL 201
           AIDS+ DM+ LF GIPLD++SVSMTMNGAVLP+LA Y+V AEEQGV  +QLAGTIQNDIL
Sbjct: 139 AIDSVEDMKILFDGIPLDKVSVSMTMNGAVLPVLAGYIVAAEEQGVSQDQLAGTIQNDIL 198

Query: 202 KEFMVRNTYIYPPQPSMRIISEIFAYTSANMPKWNSISISGYHMQEAGATADIEMAYTLA 261
           KEFMVRNTYIYPP PSM+II +I AY S NMPK+N+ISISGYH+QEAGA A +E+AYTLA
Sbjct: 199 KEFMVRNTYIYPPAPSMKIIGDIIAYCSGNMPKFNTISISGYHIQEAGANAALELAYTLA 258

Query: 262 DGVDYIRAGESVGLNVDQFAPRLSFFWGIGMNFFMEVAKLRAARMLWAKLVHQFGPKNPK 321
           DG +YIR   + GL++DQFAPRLSFFWGIGMNF+ME+AK+RAAR+LWAK+V +F P NPK
Sbjct: 259 DGKEYIRTALAAGLDIDQFAPRLSFFWGIGMNFYMEIAKMRAARLLWAKIVGEFNPGNPK 318

Query: 322 SMSLRTHSQTSGWSLTAQDVYNNVVRTCIEAMAATQGHTQSLHTNSLDEAIALPTDFSAR 381
           S  LRTHSQTSGWSLT QD YNNVVRT IEAMAA  G TQSLHTN+LDEAIALPT+FSAR
Sbjct: 319 SSMLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSAR 378

Query: 382 IARNTQLFLQQESGTTRVIDPWSGSAYVEELTWDLARKAWGHIQEVEKVGGMAKAIEKGI 441
           IARNTQL +Q+ESG T V+DPW GS  +E LT D+A KAW  I+E+E+ GGMAKAIE G+
Sbjct: 379 IARNTQLIIQEESGITNVVDPWGGSYMMESLTQDIADKAWELIEEIEEAGGMAKAIETGL 438

Query: 442 PKMRIEEAAARTQARIDSGRQPLIGVNKYRLEHEPPLDVLKVDNSTVLAEQKAKLVKLRA 501
           PK++IEEAAAR QARID G   ++GVNKY L+ E  ++ L+VDN  V   Q A+L  +R 
Sbjct: 439 PKLKIEEAAARKQARIDRGDDVIVGVNKYTLDEEDAIETLEVDNQAVRESQLARLATIRG 498

Query: 502 ERDPEKVKAALDKITWAAGNPDDKDPDRNLLKLCIDAGRAMATVGEMSDALEKVFGRYTA 561
            RD   V+A L+ I  AA + +      NLL L ++A R  ATVGE+S A+E+ FGR+ A
Sbjct: 499 SRDEAAVEAILEDIYQAAVSGEG-----NLLALAVEATRRRATVGEISFAMEREFGRFNA 553

Query: 562 QIRTISGVYSKEVKNTPEVEEARELVEEFEQAEGRRPRILLAKMGQDGHDRGQKVIATAY 621
           Q +T+SGVY        E +   + +++F    GRRPR+L+ KMGQDGHDRG KV+ATA+
Sbjct: 554 QAQTVSGVYGSAFAEDEEWQGISKDIDDFVDHHGRRPRMLVCKMGQDGHDRGAKVVATAF 613

Query: 622 ADLGFDVDVGPLFQTPEETARQAVEADVHVVGVSSLAGGHLTLVPALRKELDKLGRPDIL 681
           AD+GFD+D+ P+F TPEE ARQA+E DVHVVG SSLA GH TLVP L +EL K G  DI+
Sbjct: 614 ADVGFDIDLSPMFSTPEEVARQAIENDVHVVGASSLAAGHKTLVPELVEELKKQGADDIV 673

Query: 682 ITVGGVIPEQDFDELRKDGAVEIYTPGTVIPESA 715
           +  GGVIP QD+D L   G   I+ PGT IP  A
Sbjct: 674 VIAGGVIPRQDYDYLYGAGVKCIFGPGTPIPACA 707


Lambda     K      H
   0.317    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1361
Number of extensions: 44
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 728
Length of database: 719
Length adjustment: 40
Effective length of query: 688
Effective length of database: 679
Effective search space:   467152
Effective search space used:   467152
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources