Protein WP_050655799.1 in Rhodococcus qingshengii djl-6-2
Annotation: NCBI__GCF_002893965.1:WP_050655799.1
Length: 689 amino acids
Source: GCF_002893965.1 in NCBI
Candidate for 14 steps in catabolism of small carbon sources
Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
D-fructose catabolism | fruII-ABC | hi | The fructose-specific PTS Enzyme IIABC FruA (characterized) | 82% | 100% | 1097.4 | Fructose PTS Enzyme IIBC, FruA | 43% | 397.5 |
sucrose catabolism | fruII-ABC | hi | The fructose-specific PTS Enzyme IIABC FruA (characterized) | 82% | 100% | 1097.4 | Fructose PTS Enzyme IIBC, FruA | 43% | 397.5 |
D-fructose catabolism | fruA | med | Fructose PTS Enzyme IIBC, FruA (characterized) | 43% | 96% | 397.5 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
sucrose catabolism | fruA | med | Fructose PTS Enzyme IIBC, FruA (characterized) | 43% | 96% | 397.5 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
D-mannose catabolism | manP | med | protein-Npi-phosphohistidine-D-mannose phosphotransferase (EC 2.7.1.191) (characterized) | 40% | 73% | 336.7 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
D-fructose catabolism | fruII-C | med | PTS system, fructose subfamily, IIC subunit, component of Fructose Enzyme II complex (IIAFru - IIBFru - IICFru) (based on homology) (characterized) | 43% | 93% | 272.7 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
sucrose catabolism | fruII-C | med | PTS system, fructose subfamily, IIC subunit, component of Fructose Enzyme II complex (IIAFru - IIBFru - IICFru) (based on homology) (characterized) | 43% | 93% | 272.7 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
D-fructose catabolism | fruII-A | med | Sugar hosphotransferase system IIA component, component of Fructose-specific Enzyme I-HPr-Enzyme IIABC complex, all encoded within a single operon with genes in the order: ptsC (IIC), ptsA (IIA), ptsH (HPr), ptsI (Enzyme I) and ptsB (IIB) (characterized) | 42% | 99% | 100.1 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
sucrose catabolism | fruII-A | med | Sugar hosphotransferase system IIA component, component of Fructose-specific Enzyme I-HPr-Enzyme IIABC complex, all encoded within a single operon with genes in the order: ptsC (IIC), ptsA (IIA), ptsH (HPr), ptsI (Enzyme I) and ptsB (IIB) (characterized) | 42% | 99% | 100.1 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
D-fructose catabolism | fruII-B | med | Phosphotransferase system IIB component, component of Fructose-specific Enzyme I-HPr-Enzyme IIABC complex, all encoded within a single operon with genes in the order: ptsC (IIC), ptsA (IIA), ptsH (HPr), ptsI (Enzyme I) and ptsB (IIB) (characterized) | 42% | 83% | 88.2 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
sucrose catabolism | fruII-B | med | Phosphotransferase system IIB component, component of Fructose-specific Enzyme I-HPr-Enzyme IIABC complex, all encoded within a single operon with genes in the order: ptsC (IIC), ptsA (IIA), ptsH (HPr), ptsI (Enzyme I) and ptsB (IIB) (characterized) | 42% | 83% | 88.2 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
D-ribose catabolism | fru2-IIB | med | PTS system, fructose-specific, IIB component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence (characterized) | 44% | 98% | 79 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
xylitol catabolism | fruI | lo | The fructose inducible fructose/xylitol porter, FruI (characterized) | 37% | 98% | 391 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
D-ribose catabolism | fru2-IIC | lo | PTS system, fructose-specific, IIC component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence (characterized) | 35% | 89% | 192.6 | The fructose-specific PTS Enzyme IIABC FruA | 82% | 1097.4 |
Sequence Analysis Tools
View WP_050655799.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MSDTTAAGSSGPEIISPELISLDTDLGSTKEDVIRSLAARLTAAGRASDAAGLVDAALAR
EAQSATGLPGGIAIPHCRAEAVSSASLGFARLDPKVDFGAPDGPADLVFLIAAPEGAGAE
HMKLLSSLARALVRPAFVTSLREASTPDEIVRLVDEVLNPAPKDPAAKAAAATPPAPVST
GKHATEETPAPAPAERHIVAVTACPTGIAHTYMAADSLVAAGERAGVKVHVETQGSSGST
PLDPSVIAGAAAVIFATDVGVKGRERFAGKPVVASGVKRAINEPDKMLAEALRASENPAA
ATVDGTAAAAADSDAGSVGFGTHLRQVLLTGVSYMIPFVAAGGLLIALGFLLGGYEISGP
AKDIVLNNSITNLPDGGLATYLGAVLFQIGSLAFSFLVPALAGYIAFAIADRPGIAPGFT
AGAVAVFVGAGFIGGLVGGLIAGVVALYIGRIAVPQWLRGLMPVVIIPLFATLVVGALMF
IVLGRPLAAITSGLTDWLNGLSGSSAIFLGIILGLMMCFDLGGPVNKAAYAFAVAGLNVN
DPATLRIMAAVMAAGMVPPLAMALASTVLRPKLFSEAERENGKAAWLLGSAFISEGAIPF
AAADPLRVIPSMMAGGAVTGALIMAFDVTLSAPHGGIFVFFAVGGIGWFLVSLAAGTVVA
ALAVVGAKEFFRKGPSDAELDPEIVPAAA
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory