GapMind for catabolism of small carbon sources

 

Alignments for a candidate for xylF in Rhodococcus qingshengii djl-6-2

Align 2-hydroxymuconate semialdehyde hydrolase; HMSH; EC 3.7.1.9; 2-hydroxymuconic semialdehyde hydrolase (uncharacterized)
to candidate WP_003945808.1 C1M55_RS04155 alpha/beta fold hydrolase

Query= curated2:P19076
         (283 letters)



>NCBI__GCF_002893965.1:WP_003945808.1
          Length = 292

 Score =  154 bits (388), Expect = 3e-42
 Identities = 100/271 (36%), Positives = 145/271 (53%), Gaps = 17/271 (6%)

Query: 19  IRTNLHDSGAGFP--LMMIHGSGPGVTAWANWRLVMPELAKSRRVIAPDMLGFGYSERPA 76
           ++ + H++G G    ++++HG GPG ++W+N+   +P LAKS  VIA D  G+G S++P 
Sbjct: 23  LKLHFHEAGVGNDKTIVLLHGGGPGASSWSNFARNIPVLAKSFHVIAVDQPGYGQSDKPT 82

Query: 77  DAQYNRDVWVDHAVGVLDALEIE-QADLVGNSFGGGIALALAIRHPERVRRLVLMGSAGV 135
           +              +LD LEI  + DL+GNS GGG A+  A+ +P+R  RLVLMG  G+
Sbjct: 83  EHPQYFVHSASALNDLLDTLEITGRVDLLGNSLGGGAAVRFALDYPDRAGRLVLMGPGGL 142

Query: 136 SFPI-----TEGLDAV--WGYNPSFAEMRRLLDIFAFDRNLVNDELAELRYQASIRPGFH 188
           S  +     TEG+  +  + Y P+   +   L I  FD+ L+  EL + R+ A+  P   
Sbjct: 143 SVNLFAPDPTEGVKNLGKFSYQPTRENLEAFLRIMVFDQKLITPELVDERFAAASTP--- 199

Query: 189 ESFAAMFPAPRQRWVDG---LASAEAAIRALPHETLVIHGREDQIIPLQTSLTLADWIAR 245
           ES AA   A  + +  G   L         L    L+I GRED++ PL  +L     I R
Sbjct: 200 ESLAAA-KAMGKSFSSGDFELGMLWREAYKLRQRVLLIWGREDRVNPLDGALVALKMIPR 258

Query: 246 AQLHVFGQCGHWTQIEHAARFASLVGDFLAE 276
           AQLHVFG CGHW Q+E    F  L  DFL +
Sbjct: 259 AQLHVFGGCGHWAQLEKFDEFNRLATDFLLD 289


Lambda     K      H
   0.323    0.137    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 255
Number of extensions: 16
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 283
Length of database: 292
Length adjustment: 26
Effective length of query: 257
Effective length of database: 266
Effective search space:    68362
Effective search space used:    68362
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory