Alignments for a candidate for liuB in Rhodococcus qingshengii djl-6-2

GapMind for catabolism of small carbon sources

Alignments for a candidate for liuB in Rhodococcus qingshengii djl-6-2

Align Methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized)
to candidate WP_050656664.1 C1M55_RS20120 ATP-grasp domain-containing protein

Query= reanno::Smeli:SM_b21124
         (662 letters)



>NCBI__GCF_002893965.1:WP_050656664.1
          Length = 1827

 Score =  378 bits (970), Expect = e-108
 Identities = 259/674 (38%), Positives = 362/674 (53%), Gaps = 47/674 (6%)

Query: 1   MFSKLLIANRGEIACRIIRTARRL------GIRTVAVYSDADGDALHVALADEAIRIGGA 54
           MF ++ + NRGE A R+IR  R L      GIR +A++++A+  A+ V  ADE + +   
Sbjct: 1   MFKRIAVVNRGEAAVRLIRAVRELNAEHDYGIRVIALHTEAERRAMFVRQADEGVTLRST 60

Query: 55  PAAESYLASAPIVQAARSVGAQAIHPGYGFLSENADFAEAVAEAGMIFVGPPPAAIRAMG 114
                YL  A + +A  +  A A+  G+GF++E+  FAE VA+ G+ F+GP   ++R +G
Sbjct: 61  GTGSPYLDYAELERALLAAKADAVWVGWGFVAEDPAFAEIVAKLGITFIGPSADSMRLLG 120

Query: 115 LKDAAKALMERSGVPVVPGYHGEEQDASFLADRAREIGYPVLIKARAGGGGKGMRRVERQ 174
            K AAK L E+ GVPV P   G  +  +     A+ IGYP++IKAR+GGGG+G+R+V  +
Sbjct: 121 DKVAAKILAEKVGVPVAPWSGGPVETRADARRHAQSIGYPLIIKARSGGGGRGIRKVWAE 180

Query: 175 EDFGPALEAARREAESAFGDGSVLLERYLTKPRHIEMQVFGDRHGNIVHLFERDCSLQRR 234
           ++   ALE  + EAE +FGD  V LER +T  RH+E+QV  D HGN+     RDCS+QRR
Sbjct: 181 DELEVALERTQGEAERSFGDPVVFLERLVTDARHVEVQVIADNHGNVWAPGVRDCSIQRR 240

Query: 235 HQKVIEEAPAPGMTAEVRRAMGDAAVRAAQAIGYVGAGTVEFIADVTNGLWPDHFYFMEM 294
           +QKVIEE+ +P +T E    +   +    +A GY GAGTVE++    N L    F F+E+
Sbjct: 241 NQKVIEESASPVLTEEQSDHLKKVSAELVKAAGYQGAGTVEYLYQPENKL----FTFLEV 296

Query: 295 NTRLQVEHPVTEAITGIDLVEWQLRVASGEPLPKKQADISMNGWAFEARLYAEDPARGFL 354
           NTRLQVEHP+TE  TGIDLV+ Q+ VASG+ L          G A EARL AED    F 
Sbjct: 297 NTRLQVEHPITEVTTGIDLVKLQILVASGDELVGDCPPAF--GHAVEARLNAEDADNDFA 354

Query: 355 PATGRLTELSFPEGTS-RVDSGVRQGDTITPYYDPLIAKLIVHGQNRSAALGRLQDALKE 413
           PA G +  L FP G+  RVD+G+  GD I P YD ++AK+I  G+NRS AL RL+ AL+E
Sbjct: 355 PAPGTVQLLKFPLGSGIRVDTGIAAGDVIPPDYDSMVAKVIAWGRNRSEALARLRTALRE 414

Query: 414 CRI---GGTVTNRDFLIRLTEEHDFRSGHPDTGLIDREIERLTAPVAPGDEALALAAIFS 470
             +   GGT T + FL+ L +  +  S   DTG +DR    LT      D AL   AI  
Sbjct: 415 TTVVLDGGT-TTKSFLLDLLDRDEVISASADTGWLDR-TGALTGTTRRADVALIATAI-- 470

Query: 471 TGALDPNRSTDPWSSL-----GSWQIWGDAHRMVVIEHADVRATVTLASRGRDQFAVRAG 525
             A D   S +  + L     G  +      R V + +      +T+   G  ++ V   
Sbjct: 471 -DAYDAEESLERAAFLASARGGRPRANHAIGRTVELNYQGQAYKLTVGRIGPHRYRVDGD 529

Query: 526 ASTLPVLV--LDRFEGGARLEVAGQKRLIRFSRDREALTLFHGGRNLVFHVPDGLTGGQS 583
           A  + V V  L  FE           RL+   R    +++    R LV         G S
Sbjct: 530 AGDIEVDVDRLGDFE----------SRLVIGDRRFHVVSVVSPARYLV------EVDGIS 573

Query: 584 SEIADDE---LVAPMPGLVKLVRVGAGDAVTKGQALVVMEAMKMELTLSASREGTIANVH 640
            +I+ D+   + AP P +V  V V  GD V  G  LVV+E+MKME  + A   G +  V 
Sbjct: 574 HQISQDDAGVVRAPAPAVVVAVPVAVGDDVEAGSTLVVLESMKMETAVRAPYAGKVREVL 633

Query: 641 VAEGAQVSEGTVLV 654
               +QV  G  L+
Sbjct: 634 ATVNSQVDAGAPLL 647


Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2837
Number of extensions: 134
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 662
Length of database: 1827
Length adjustment: 46
Effective length of query: 616
Effective length of database: 1781
Effective search space:  1097096
Effective search space used:  1097096
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources