Alignments for a candidate for aacS in Rhodococcus qingshengii djl-6-2

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Rhodococcus qingshengii djl-6-2

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate WP_039973143.1 C1M55_RS09195 AMP-binding protein

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>NCBI__GCF_002893965.1:WP_039973143.1
          Length = 545

 Score =  602 bits (1552), Expect = e-176
 Identities = 302/564 (53%), Positives = 377/564 (66%), Gaps = 24/564 (4%)

Query: 10  SAPLVDSHARGATDVPLIEQTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRL 69
           S PL  S+  G  D P++  TIG      VA   +R+AL+    GRR+TY +   + + L
Sbjct: 2   STPL-PSYTSGVWDGPMLGDTIGDNLDRTVAAHGDRDALIDHASGRRWTYREFAEQVNGL 60

Query: 70  ASALLGMGLTPGDRVGIWSHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGC 129
           A+ LL  G+  GDRVGIW+ N  EW   Q ATA+VG +LVNINPAYR+ E++Y L + G 
Sbjct: 61  AAGLLSRGVGKGDRVGIWAPNCPEWTFTQYATAKVGAILVNINPAYRSHELQYVLEQAGI 120

Query: 130 KLLVSMARFKTSDYLGMLRELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGL 189
             LVS A FKTSDY  M+  + P+              P L +V+ +           GL
Sbjct: 121 STLVSAASFKTSDYASMIETVRPQ-------------CPDLTSVLLLGSPEWDAVLADGL 167

Query: 190 LRFTELIARGNAADPR-LAQVAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFI 248
                      A+DP  LA   A L A D INIQ+TSGTTGFPKGATL+H NILNNG+F+
Sbjct: 168 A--------AQASDPAPLAAAQAALSADDAINIQYTSGTTGFPKGATLSHHNILNNGYFV 219

Query: 249 GECMKLTPADRLCIPVPLYHCFGMVLGNLACFTHGATIVYPNDGFDPLTVLQTVQDERCT 308
           GE    +  DR+CIPVP YHCFGMV+GNLAC +HGA +V P   FDP   L+ VQ E+CT
Sbjct: 220 GELCHYSEVDRVCIPVPFYHCFGMVMGNLACTSHGAAMVIPGPAFDPRASLEAVQAEKCT 279

Query: 309 GLHGVPTMFIAELDHPRFAEFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMT 368
            L+GVPTMFIAEL  P F  F+LS+LRTGIMAGSPCP EVMK+V+++M + E++I YGMT
Sbjct: 280 SLYGVPTMFIAELALPDFDSFDLSSLRTGIMAGSPCPVEVMKQVIDRMGMSEVSICYGMT 339

Query: 369 ETSPVSCQSSTDTPLSKRVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHG 428
           ETSPVS Q+ +D  + +R  TVG+V PHLE+KIVDP TG  VP G+ GE CT+GYSVM G
Sbjct: 340 ETSPVSLQTRSDDSIEQRTETVGRVGPHLEIKIVDPATGLTVPRGEPGELCTRGYSVMLG 399

Query: 429 YWGDEAKTREAIDEGGWMHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYR 488
           YW +  KT EAID   WMHTGD+  MD  GYV I GRIKDMVIRGGEN+YPREIEEFLY 
Sbjct: 400 YWENPEKTAEAIDAARWMHTGDIGVMDEAGYVAITGRIKDMVIRGGENVYPREIEEFLYT 459

Query: 489 HPQVQDVQVVGVPDQKYGEELCAWIIAKPGTQPTE-DDIRAFCKGQIAHYKVPRYIRFVT 547
           HP + D QV+GVPD KYGEEL  W+  + G    + D +RAFC G++AHYK+PRY+  V 
Sbjct: 460 HPDILDAQVIGVPDAKYGEELMVWVQMREGADDLDADSVRAFCTGKLAHYKIPRYVHVVD 519

Query: 548 SFPMTVTGKIQKFKIRDEMKDQLG 571
            FPMTVTGK++K  +R++  + +G
Sbjct: 520 EFPMTVTGKVRKIAMREQAIELIG 543


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 873
Number of extensions: 28
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 545
Length adjustment: 36
Effective length of query: 542
Effective length of database: 509
Effective search space:   275878
Effective search space used:   275878
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources