GapMind for catabolism of small carbon sources

 

D-ribose catabolism in Oceanisphaera arctica V1-41

Best path

rbsA, rbsB, rbsC, rbsK

Rules

Overview: Ribose degradation in GapMind is based on the MetaCyc pathway ribose phosphorylation (link), which yields the central metabolic intermediate D-ribofuranose 5-phosphate, or on uptake by a phosphotransferase system.

14 steps (8 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
rbsA D-ribose ABC transporter, ATPase component RbsA UN63_RS08345
rbsB D-ribose ABC transporter, substrate-binding component RbsB UN63_RS08335
rbsC D-ribose ABC transporter, permease component RbsC UN63_RS08340
rbsK ribokinase UN63_RS08330
Alternative steps:
BT2809 D-ribose transporter
frcA D-ribose ABC transporter, ATPase component FrcA UN63_RS08345 UN63_RS06410
frcB D-ribose ABC transporter, substrate-binding component FrcB
frcC D-ribose ABC transporter, permease component FrcC UN63_RS08340
fru2-IIA D-ribose PTS, IIA component
fru2-IIB D-ribose PTS, IIB component UN63_RS01635
fru2-IIC D-ribose PTS, IIC component UN63_RS01635
LmGT2 D-ribose transporter LmGT2
PLT5 D-ribose transporter PLT5
rbsU probable D-ribose transporter RbsU

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory