Align 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 (characterized)
to candidate WP_106711729.1 CU102_RS14130 acetyl-CoA C-acetyltransferase
Query= SwissProt::P0C7L2 (401 letters) >NCBI__GCF_003010955.1:WP_106711729.1 Length = 402 Score = 246 bits (629), Expect = 7e-70 Identities = 167/426 (39%), Positives = 227/426 (53%), Gaps = 49/426 (11%) Query: 1 MREAFICDGIRTPIGR--YGGALSSVRADDLAAIPLRELLVRNPRLDAECIDDVILGCAN 58 M EA+I D +RTP GR G+L V A L A L L RN LD +DD+I GC + Sbjct: 1 MTEAYIYDHVRTPRGRGKKDGSLHEVPAVRLGAHVLEALRDRNG-LDTGLVDDIIYGCVD 59 Query: 59 QAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAARAIKAGDGDLLIAGGVE 118 GE + R + AG G I+R C SGLDA+ FAA I G D++IAGGVE Sbjct: 60 PVGEAGAVIPRSSAFEAGYDFKAPGMQISRFCASGLDAVNFAAAKIVQGADDIVIAGGVE 119 Query: 119 SMSRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGTDSMPE--TAENVAELLKI 176 SMSR MG + A+ D ++G + F MP+ +A+ +A Sbjct: 120 SMSRVG--MGMSGGAW-----YMDPSVG-------LPGYF----MPQGVSADLIATKYGF 161 Query: 177 SREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVTEIQHDEHLRPETTLEQLR 236 SR+D D++A+ SQ+R +A G ++ + K++ G+ T + HDEH+RP T ++ L Sbjct: 162 SRDDVDAYAVESQKRAGEAWKKGYFKNSVLEI--KDQNGL-TILDHDEHMRPTTDMQALA 218 Query: 237 GLKAPF---------------------RANGVITAGNASGVNDGAAALIIASEQMAAAQG 275 L F N V AGN+SG+ DGAA +++ S+ A G Sbjct: 219 SLNPSFVMPGEMGGFNAVGIQAHPEVETINHVHHAGNSSGIVDGAAGVLLGSKSAGKAIG 278 Query: 276 LTPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVLR 335 PRARI A A G +P LM GPV T ++L+RA + I D+D+ ELNEAFAA L ++ Sbjct: 279 TKPRARIRAFANIGSDPALMLTGPVDVTEKLLKRAKMKISDIDLFELNEAFAAVVLRYMQ 338 Query: 336 ELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQGIA 395 +P D +N NGGAIA+GHPLG +GA + EL RR+ AL T+CIG G G A Sbjct: 339 AFDIPRD--KINVNGGAIAMGHPLGATGAMILGTVLDELERRDLNVALVTLCIGAGMGTA 396 Query: 396 MILERV 401 I+ERV Sbjct: 397 TIIERV 402 Lambda K H 0.319 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 432 Number of extensions: 16 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 401 Length of database: 402 Length adjustment: 31 Effective length of query: 370 Effective length of database: 371 Effective search space: 137270 Effective search space used: 137270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory