Alignments for a candidate for fruK in Phyllobacterium brassicacearum STM 196

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruK in Phyllobacterium brassicacearum STM 196

Align Fructose import ATP-binding protein FruK; EC 7.5.2.- (characterized)
to candidate WP_106713881.1 CU102_RS25520 sugar ABC transporter ATP-binding protein

Query= SwissProt::Q8G847
         (513 letters)



>NCBI__GCF_003010955.1:WP_106713881.1
          Length = 512

 Score =  394 bits (1012), Expect = e-114
 Identities = 203/500 (40%), Positives = 322/500 (64%), Gaps = 2/500 (0%)

Query: 7   IVVMKGITIEFPGVKALDGVDLTLYPGEVHALMGENGAGKSTMIKALTGVYKINAGSIMV 66
           ++ M GI   F G+ AL G    +  GEVHAL+G+NGAGKST+IK LTG Y+ +AG +  
Sbjct: 6   LLSMTGIDKRFAGIPALSGASFAVGRGEVHALIGQNGAGKSTLIKVLTGYYRRDAGDVAF 65

Query: 67  DGKPQQFNGTLDAQNAGIATVYQEVNLCTNLSVGENVMLGHEKRGPFGIDWKKTHEAAKK 126
           DGKP +      AQ +GI+T+YQE+NL    S+ EN+ LG E R    +DWK+ H+ A+ 
Sbjct: 66  DGKPFEVFSPQLAQASGISTIYQEINLVPYRSITENICLGREARHYGLLDWKRMHKEAET 125

Query: 127 YLAQMGLESIDPHTPLSSISIAMQQLVAIARAMVINAKVLILDEPTSSLDANEVRDLFAI 186
            L +  +  +D   PL   + A QQ+VAIARA+  +AK++++DEPTSSLD  EV  LF +
Sbjct: 126 LLRRFNVR-VDVDRPLMEFNTATQQMVAIARAIGFSAKLVVMDEPTSSLDDREVTVLFDV 184

Query: 187 MRKVRDSGVAILFVSHFLDQIYEITDRLTILRNGQFIKEVMTKDTPRDELIGMMIGKSAA 246
           +R+++  GV+++FVSH LD++YE+ DR+TI+R+G+ +K     +  +  L+  M+G++  
Sbjct: 185 IRQLKQEGVSVVFVSHKLDELYEVCDRVTIMRDGRTVKTAAMTEIDKIGLVSSMLGRTIN 244

Query: 247 ELSQIGAKKARREITPGEKPIVDVKGLGKKGTINPVDVDIYKGEVVGFAGLLGSGRTELG 306
                    + R+     K ++  + L    T+  V  D+  GE+ GFAGLLG+GRTE  
Sbjct: 245 RAEGHATAFSDRDEKKIGKVLLSAQNLSINRTVQDVSFDVRSGEIAGFAGLLGAGRTETA 304

Query: 307 RLLYGADKPDSGTYTLNGKKVNISDPYTALKNKIAYSTENRRDEGIIGDLTVRQNILIAL 366
           RL++G D+P +G    +G++ +   P  A+   + + TE+R+ EGI+ +++V +N+++AL
Sbjct: 305 RLVFGIDRPRAGIMHYDGQQFDPRSPADAIAAGMGFCTEDRKSEGIVPEMSVAENMMLAL 364

Query: 367 QATRGMFKPIPKKEADAIVDKYMKELNVRPADPDRPVKNLSGGNQQKVLIGRWLATHPEL 426
                    I +K    IV++++++L+++ + PD+ V+ LSGGNQQKVL+GRWLA +P L
Sbjct: 365 MPKVSKSGVIDEKAQRKIVERFIRQLDIKCSGPDQKVRELSGGNQQKVLLGRWLAMNPTL 424

Query: 427 LILDEPTRGIDIGAKAEIQQVVLDLASQGMGVVFISSELEEVVRLSDDIEVLKDRHKIAE 486
           LILDEPTRGID+GAK EIQ ++  LA +G+ V+ ISSELEEV+  +D + VL++   +AE
Sbjct: 425 LILDEPTRGIDVGAKGEIQSLIKSLADRGLAVLMISSELEEVIEGADRVFVLREGITVAE 484

Query: 487 IENDDTVSQATIVETIANTN 506
            + +   ++  ++  +A+ N
Sbjct: 485 FDRNQ-ATEDVLMSAMAHGN 503



 Score = 89.4 bits (220), Expect = 3e-22
 Identities = 60/233 (25%), Positives = 112/233 (48%), Gaps = 5/233 (2%)

Query: 21  KALDGVDLTLYPGEVHALMGENGAGKSTMIKALTGVYKINAGSIMVDGKPQQFNGTLDAQ 80
           + +  V   +  GE+    G  GAG++   + + G+ +  AG +  DG+        DA 
Sbjct: 275 RTVQDVSFDVRSGEIAGFAGLLGAGRTETARLVFGIDRPRAGIMHYDGQQFDPRSPADAI 334

Query: 81  NAGIATVYQE---VNLCTNLSVGENVMLG-HEKRGPFG-IDWKKTHEAAKKYLAQMGLES 135
            AG+    ++     +   +SV EN+ML    K    G ID K   +  ++++ Q+ ++ 
Sbjct: 335 AAGMGFCTEDRKSEGIVPEMSVAENMMLALMPKVSKSGVIDEKAQRKIVERFIRQLDIKC 394

Query: 136 IDPHTPLSSISIAMQQLVAIARAMVINAKVLILDEPTSSLDANEVRDLFAIMRKVRDSGV 195
             P   +  +S   QQ V + R + +N  +LILDEPT  +D     ++ ++++ + D G+
Sbjct: 395 SGPDQKVRELSGGNQQKVLLGRWLAMNPTLLILDEPTRGIDVGAKGEIQSLIKSLADRGL 454

Query: 196 AILFVSHFLDQIYEITDRLTILRNGQFIKEVMTKDTPRDELIGMMIGKSAAEL 248
           A+L +S  L+++ E  DR+ +LR G  + E        D L+  M   +A  +
Sbjct: 455 AVLMISSELEEVIEGADRVFVLREGITVAEFDRNQATEDVLMSAMAHGNAPRI 507


Lambda     K      H
   0.316    0.135    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 614
Number of extensions: 26
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 513
Length of database: 512
Length adjustment: 35
Effective length of query: 478
Effective length of database: 477
Effective search space:   228006
Effective search space used:   228006
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources