Align Carbon starvation protein A (characterized, see rationale)
to candidate WP_095190351.1 C0J26_RS26670 carbon starvation protein A
Query= uniprot:A0A0N9VZ52 (685 letters) >NCBI__GCF_003031005.1:WP_095190351.1 Length = 688 Score = 1267 bits (3278), Expect = 0.0 Identities = 631/682 (92%), Positives = 660/682 (96%) Query: 4 LAKHLAWFAVAVLGAFALSVVALRRGEAINALWIVVAAVAIYLVAYRYYSLFIANKVMQL 63 L +HL W +A++GA AL VVALRRGEAINALWIVVAAVAIYLVAYRYYSLFIAN VMQL Sbjct: 7 LLRHLPWLVLAIVGACALGVVALRRGEAINALWIVVAAVAIYLVAYRYYSLFIANHVMQL 66 Query: 64 DPGRATPAVVNNDGLDYVPTNKHILFGHHFAAIAGAGPLVGPVLAAQMGYLPGTLWLIAG 123 DP RATPAV+NNDGLDYVPTNKHILFGHHFAAIAGAGPLVGPVLAAQMGYLPGTLWLIAG Sbjct: 67 DPRRATPAVLNNDGLDYVPTNKHILFGHHFAAIAGAGPLVGPVLAAQMGYLPGTLWLIAG 126 Query: 124 VVLAGAVQDFMVLFMSTRRNGRSLGDMVREEMGRVPGTIALFGCFLIMIIILAVLALIVV 183 VVLAGAVQDFMVLFMSTRRNGRSLGDMVREEMGRVPGTIALFGCFLIMIIILAVLALIVV Sbjct: 127 VVLAGAVQDFMVLFMSTRRNGRSLGDMVREEMGRVPGTIALFGCFLIMIIILAVLALIVV 186 Query: 184 KALADSPWGMFTVMATIPIAMFMGVYMRYIRPGRIGEISVVGVLLLLGSIWLGGQIAADP 243 KALA+SPWG+FTVMATIPIAMFMG+YMRYIRPGRIGEISVVGVLLLLGSIWLGGQIAADP Sbjct: 187 KALAESPWGIFTVMATIPIAMFMGIYMRYIRPGRIGEISVVGVLLLLGSIWLGGQIAADP 246 Query: 244 VWAKAFSFTGIQITWMLIGYGFVAAVMPVWLILAPRDYLSTFLKIGTIMALAIGILITMP 303 VWAKAF+FTG+QITWML+GYGFVAA +PVWLILAPRDYLSTFLKIGTI+ALAIGIL+TMP Sbjct: 247 VWAKAFTFTGVQITWMLVGYGFVAASLPVWLILAPRDYLSTFLKIGTIVALAIGILVTMP 306 Query: 304 ELKMPALTQFIDGTGPVWKGGLFPFLFITIACGAVSGFHALIASGTTPKLLASEGHARYI 363 ELKMPALTQF+DGTGPVWKGGLFPFLFITIACGAVSGFHALI+SGTTPKLL +E +ARYI Sbjct: 307 ELKMPALTQFVDGTGPVWKGGLFPFLFITIACGAVSGFHALISSGTTPKLLDNETNARYI 366 Query: 364 GYGGMLMESFVAIMAMVAASVIEPGVYFAMNSPAAIVGGDVVQVAQTVSSWGFMITPEAL 423 GYGGMLMESFVAIMAMVAASVIEPGVYFAMNSPAA+VG DVV VAQ V+SWGF ITPEAL Sbjct: 367 GYGGMLMESFVAIMAMVAASVIEPGVYFAMNSPAAVVGSDVVSVAQVVTSWGFAITPEAL 426 Query: 424 QAVAKDIGETTVLARAGGAPTLAVGIAQILHSVLPGENTMAFWYHFAILFEALFILTAVD 483 QAVA DIGETT+LARAGGAPTLAVGIAQILHSVLPGENTMAFWYHFAILFEALFILTAVD Sbjct: 427 QAVAHDIGETTILARAGGAPTLAVGIAQILHSVLPGENTMAFWYHFAILFEALFILTAVD 486 Query: 484 AGTRAGRFMLQDLLGSFVPALKRTESWTANLIATAGCVAMWGYLLYQGVIDPLGGINTLW 543 AGTRAGRFMLQDLLGSFVPALKRTESWTANLIATAGCVAMWG+LLYQGVIDPLGGINTLW Sbjct: 487 AGTRAGRFMLQDLLGSFVPALKRTESWTANLIATAGCVAMWGWLLYQGVIDPLGGINTLW 546 Query: 544 PLFGISNQMLAGIALMLSTVVLIKMKRQRYIWVTLLPAAWLLICTTTAGFIKLFDANPAI 603 PLFGISNQMLAGIALML TVVLIKMKRQRYIWVTLLPA WLLICTTTAGFIKLFDANPAI Sbjct: 547 PLFGISNQMLAGIALMLGTVVLIKMKRQRYIWVTLLPATWLLICTTTAGFIKLFDANPAI 606 Query: 604 GFLSLAKKYSDALANGQILAPAKDITQMQHVIYNAYTNATLTALFLFVVFSILFYAIKVG 663 GFLSLAKKYSDALANGQ+LAPAK + QMQHVI+NAYTNATLT LFLFVVFSILFYA+KVG Sbjct: 607 GFLSLAKKYSDALANGQVLAPAKSVEQMQHVIFNAYTNATLTVLFLFVVFSILFYALKVG 666 Query: 664 ISAWGSKERTDKESPFQAIPEA 685 I+AWG KERTDKESPFQA+P+A Sbjct: 667 IAAWGKKERTDKESPFQALPDA 688 Lambda K H 0.328 0.141 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1652 Number of extensions: 45 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 685 Length of database: 688 Length adjustment: 39 Effective length of query: 646 Effective length of database: 649 Effective search space: 419254 Effective search space used: 419254 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory