GapMind for catabolism of small carbon sources

 

D-cellobiose catabolism in Rhodobacter johrii JA192

Best path

bgl, aglE', aglF', aglG', aglK', glk

Rules

Overview: MetaCyc does not list any pathways for cellobiose utilization, but the major catabolic enzymes are believed to be intracellular cellobiase, periplasmic cellobiase, cellobiose-6-phosphate hydrolase, or cellobiose phosphorylase (PMID:28535986). These pathways all lead to glucose-6-phosphate, which is a central metabolic intermediate. There also may be a 3-ketoglucoside pathway in some Bacteroidetes, but this is not characterized.

73 steps (37 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
bgl cellobiase C8J29_RS06000
aglE' glucose ABC transporter, substrate-binding component (AglE) C8J29_RS05990
aglF' glucose ABC transporter, permease component 1 (AglF) C8J29_RS05985 C8J29_RS19300
aglG' glucose ABC transporter, permease component 2 (AglG) C8J29_RS05980 C8J29_RS18655
aglK' glucose ABC transporter, ATPase component (AglK) C8J29_RS05975 C8J29_RS18590
glk glucokinase C8J29_RS06005 C8J29_RS19290
Alternative steps:
ascB 6-phosphocellobiose hydrolase C8J29_RS06000
bglF glucose PTS, enzyme II (BCA components, BglF)
bglG cellobiose PTS system, EII-BC or EII-BCA components
bglT cellobiose transporter BglT
cbp cellobiose phosphorylase
cbpB cellobiose ABC transporter, substrate-binding component CpbB
cbpC cellobiose ABC transporter, substrate-binding component CbpC
cbtA cellobiose ABC transporter, substrate-binding component CbtA
cbtB cellobiose ABC transporter, permease component 1 (CbtB) C8J29_RS14025 C8J29_RS18785
cbtC cellobiose ABC transporter, permease component 2 (CbtC) C8J29_RS19490
cbtD cellobiose ABC transporter, ATPase component 1 (CbtD) C8J29_RS18795 C8J29_RS21130
cbtF cellobiose ABC transporter, ATPase component 2 (CbtF) C8J29_RS21130 C8J29_RS13945
cdt cellobiose transporter cdt-1/cdt-2
cebE cellobiose ABC transporter, substrate-binding component CebE
cebF cellobiose ABC transporter, permease component 1 (CebF) C8J29_RS18650
cebG cellobiose ABC transporter, permease component 2 (CebG) C8J29_RS19305 C8J29_RS20195
celEIIA cellobiose PTS system, EII-A component
celEIIB cellobiose PTS system, EII-B component
celEIIC cellobiose PTS system, EII-C component
crr glucose PTS, enzyme IIA
eda 2-keto-3-deoxygluconate 6-phosphate aldolase C8J29_RS04885 C8J29_RS14230
edd phosphogluconate dehydratase C8J29_RS04890 C8J29_RS13180
gadh1 gluconate 2-dehydrogenase flavoprotein subunit
gadh2 gluconate 2-dehydrogenase cytochrome c subunit C8J29_RS05500
gadh3 gluconate 2-dehydrogenase subunit 3
gdh quinoprotein glucose dehydrogenase C8J29_RS05030 C8J29_RS18500
glcS glucose ABC transporter, substrate-binding component (GlcS)
glcT glucose ABC transporter, permease component 1 (GlcT)
glcU glucose ABC transporter, permease component 2 (GlcU)
glcU' Glucose uptake protein GlcU
glcV glucose ABC transporter, ATPase component (GclV) C8J29_RS07090 C8J29_RS18660
gnl gluconolactonase
gtsA glucose ABC transporter, substrate-binding component (GtsA)
gtsB glucose ABC transporter, permease component 1 (GtsB) C8J29_RS14165 C8J29_RS05985
gtsC glucose ABC transporter, permease component 2 (GtsC) C8J29_RS05980 C8J29_RS18190
gtsD glucose ABC transporter, ATPase component (GtsD) C8J29_RS18660 C8J29_RS14155
kguD 2-keto-6-phosphogluconate reductase C8J29_RS14545 C8J29_RS03220
kguK 2-ketogluconokinase C8J29_RS09170
kguT 2-ketogluconate transporter
manX glucose PTS, enzyme EIIAB
manY glucose PTS, enzyme EIIC
manZ glucose PTS, enzyme EIID
MFS-glucose glucose transporter, MFS superfamily
mglA glucose ABC transporter, ATP-binding component (MglA) C8J29_RS16910 C8J29_RS16570
mglB glucose ABC transporter, substrate-binding component C8J29_RS16900 C8J29_RS17285
mglC glucose ABC transporter, permease component (MglC) C8J29_RS16905 C8J29_RS17225
msdB1 cellobiose ABC transporter, permease component 1 (MsdB1) C8J29_RS20190 C8J29_RS18650
msdB2 cellobiose ABC transporter, permease component 2 (MsdB2) C8J29_RS20195 C8J29_RS18190
msdC1 cellobiose ABC transporter, permease component 1 (MsdC1)
msdC2 cellobiose ABC transporter, permease component 1 (MsdC2) C8J29_RS05980
msiK cellobiose ABC transporter, ATPase component C8J29_RS18185 C8J29_RS14155
PAST-A proton-associated sugar transporter A
pgmA alpha-phosphoglucomutase C8J29_RS06045 C8J29_RS10995
ptsG glucose PTS, enzyme IICB
ptsG-crr glucose PTS, enzyme II (CBA components, PtsG)
SemiSWEET Sugar transporter SemiSWEET
SMc04256 cellobiose ABC transporter, ATPase component C8J29_RS18660 C8J29_RS18185
SMc04257 cellobiose ABC transporter, permease component 1 C8J29_RS05980 C8J29_RS18190
SMc04258 cellobiose ABC transporter, permease component 2
SMc04259 cellobiose ABC transporter, substrate-binding protein
SSS-glucose Sodium/glucose cotransporter
SWEET1 bidirectional sugar transporter SWEET1
TM0027 cellobiose ABC transporter, ATPase component 2 C8J29_RS10245 C8J29_RS13945
TM0028 cellobiose ABC transporter, ATPase component 1 C8J29_RS18800 C8J29_RS21135
TM0029 cellobiose ABC transporter, permease component 2 C8J29_RS19350
TM0030 cellobiose ABC transporter, permease component 1 C8J29_RS13930 C8J29_RS21175
TM0031 cellobiose ABC transporter, substrate-binding component

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory