Alignments for a candidate for aglE in Rhodobacter johrii JA192

GapMind for catabolism of small carbon sources

Alignments for a candidate for aglE in Rhodobacter johrii JA192

Align AglE aka R00695 aka SMC03061, component of Sucrose/maltose/trehalose porter (sucrose-inducible) (characterized)
to candidate WP_069331924.1 C8J29_RS05990 carbohydrate ABC transporter substrate-binding protein

Query= TCDB::Q9Z3R5
         (458 letters)



>NCBI__GCF_003046325.1:WP_069331924.1
          Length = 450

 Score =  640 bits (1652), Expect = 0.0
 Identities = 299/446 (67%), Positives = 350/446 (78%), Gaps = 2/446 (0%)

Query: 13  ALLAGTAGLAGTAGAADLKFKPGEDSRFNWASLEEFKKGHDLKGQTLTIFGPWRGEDEAL 72
           A+ A  A ++G     +LKF PG D+RF+W S E  K G DL GQ LTI GPW G D+AL
Sbjct: 7   AIAAALALVSGPLAGQELKFPPGADARFHWDSFEALK-GQDLSGQRLTILGPWLGPDKAL 65

Query: 73  FKSVYAYFVEATGVELKYSSSENYEQQIVIDTQAGSPPDVAILPQPGLIADLAAKGLLTP 132
           F SV AYF  ATG E+ Y+ S+N+EQQIVID  AGSPPD+A+ PQPGL  DLA+KG L P
Sbjct: 66  FDSVIAYFEAATGAEVTYNGSDNFEQQIVIDAGAGSPPDIAVFPQPGLARDLASKGQLAP 125

Query: 133 LGDETKQWLLDNYAAGQSWVDLSTYNGKDGTSALYAFPYKIDVKSLVWYVPENFEDAGYE 192
           L   T  WL +NYAAG+SWV L T+ G+DGT ALY F YKIDVKSLVWYVPENF D GYE
Sbjct: 126 LDPATADWLRENYAAGESWVSLGTFPGRDGTEALYGFFYKIDVKSLVWYVPENFADFGYE 185

Query: 193 VPKTMEELKALTEKIAEDGEKPWCIGLGSGGATGWPATDWVEDLMLRTQPAETYDKWVKN 252
           +P TMEEL+ALTE++  DG  PWC+GL SGGATGWPATDWVED+MLR  P E YD+W  N
Sbjct: 186 IPATMEELRALTERMVADGVTPWCVGLASGGATGWPATDWVEDMMLRINPPEVYDRWTSN 245

Query: 253 EIPFTDAAVTGALEEFGWFARNDAFVDGGAAAVASTDFRDSPKGLFSSPPKCYLHHQASF 312
           EIPF D  V  A+EEFGWFAR   FV GG  AVA+TDFRDSPKGLFS+PP+C++H QASF
Sbjct: 246 EIPFDDPQVVAAIEEFGWFARTPDFVAGGPNAVAATDFRDSPKGLFSAPPQCFMHKQASF 305

Query: 313 IPSFFPEGKVVGEDADFFYMPPYESKKELGNPVLGAGTLAMITKDTPAARAFIEFLKTPI 372
           IPSFFPEG V+GEDADFFY+P Y + ++LG PVLGAGT+  +T+DTP ARAF+EFLKTPI
Sbjct: 306 IPSFFPEGTVIGEDADFFYLPAY-ADRDLGQPVLGAGTVFGLTRDTPVARAFLEFLKTPI 364

Query: 373 AHEVWMAQTSFLTPYKSVNVDVYGNPPLKKQGEILLNATTFRFDGSDLMPGKIGAGAFWT 432
           AHEVWMAQT FLTP+  VN DVYG+P L+K GEILLNATTFRFDGSDLMPG +GAGAFWT
Sbjct: 365 AHEVWMAQTGFLTPFTGVNTDVYGDPTLRKMGEILLNATTFRFDGSDLMPGAVGAGAFWT 424

Query: 433 GMVDFVGGKSSADVAAGVQKAWDSIK 458
           GM+D++GGK +  V A +Q+ WD+ K
Sbjct: 425 GMIDYMGGKGAERVGADIQRTWDTFK 450


Lambda     K      H
   0.316    0.135    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 805
Number of extensions: 39
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 458
Length of database: 450
Length adjustment: 33
Effective length of query: 425
Effective length of database: 417
Effective search space:   177225
Effective search space used:   177225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources