GapMind for catabolism of small carbon sources

 

Alignments for a candidate for galdh in Limnohabitans curvus MWH-C5

Align L-arabinose 1-dehydrogenase / D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (characterized)
to candidate WP_108358580.1 B9Z44_RS13785 3-oxoacyl-ACP reductase FabG

Query= reanno::pseudo13_GW456_L13:PfGW456L13_2119
         (272 letters)



>NCBI__GCF_003063475.1:WP_108358580.1
          Length = 255

 Score =  151 bits (381), Expect = 2e-41
 Identities = 90/251 (35%), Positives = 136/251 (54%), Gaps = 6/251 (2%)

Query: 15  RGERLKNKVVLLTGAAQGIGEAIVATFASQQARLIISDIQGEKVEKVAAHWRDQGADVVA 74
           + +RL+ KV L+TGAAQGIG A    FA + A +I+ D++   V++     +  GA  + 
Sbjct: 8   QAQRLQGKVSLITGAAQGIGLATALKFAREGAIVIVCDVKQAAVDEAVKQCQALGAQALG 67

Query: 75  IKADVSRQQDLHAMARLAIDLHGRIDVLVNCAGVNVFRDPLEMTEEDWRRCFAIDLDGAW 134
              DV+++  + A  +  +D  GRIDVLVN AG+       +MT E + R   ++L G +
Sbjct: 68  FVVDVTQRDMVDATVKAVLDKFGRIDVLVNNAGITQDARLQKMTLEQFDRVIDVNLRGVF 127

Query: 135 YGCKAVLPQMIEQGIGSIINIASTHSTHIIPGCFPYPVAKHGLLGLTRALGIEYAPKGVR 194
           +  +AV   M+ QG G I+N +S    +   G   Y   K G++G T+    E  PKG+R
Sbjct: 128 HCAQAVTEAMVAQGSGVILNASSVVGIYGNFGQTNYAATKFGVIGFTKTWSRELGPKGIR 187

Query: 195 VNAIAPGYIETQLNVDYWNGFADPHAERQRAFDLHPPRRIGQPIEVAMTAVFLASDEAPF 254
           VNA+APG+I+T +          P        D  P +R+GQP ++A    FLASDEA +
Sbjct: 188 VNAVAPGFIQTPILSTI------PEKVIHEMTDRVPLKRLGQPEDIANVYAFLASDEAAY 241

Query: 255 INASCITIDGG 265
           IN + I + GG
Sbjct: 242 INGTVIEVAGG 252


Lambda     K      H
   0.322    0.138    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 130
Number of extensions: 5
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 272
Length of database: 255
Length adjustment: 25
Effective length of query: 247
Effective length of database: 230
Effective search space:    56810
Effective search space used:    56810
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory