Alignments for a candidate for treEIIA in Limnohabitans curvus MWH-C5

GapMind for catabolism of small carbon sources

Alignments for a candidate for treEIIA in Limnohabitans curvus MWH-C5

Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate WP_108360283.1 B9Z44_RS08055 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::pseudo3_N2E3:AO353_15995
         (844 letters)



>NCBI__GCF_003063475.1:WP_108360283.1
          Length = 586

 Score =  262 bits (670), Expect = 4e-74
 Identities = 189/583 (32%), Positives = 287/583 (49%), Gaps = 39/583 (6%)

Query: 277 LRGVCASAGSAFGYVVQVAERTLEMPEFAADQQLERESLERALMHATQA----LQRLRDN 332
           + G+  + G A G  V VA   +++  +  +       +ER L  A QA    LQ+L DN
Sbjct: 5   VHGLAVARGIAIGRAVLVASSRVDVAHYFIEATQVASEIER-LSEARQAVMAELQQLHDN 63

Query: 333 AAGEAQ---ADIFKAHQELLEDPSLLEQAQALIAEGK-SAAFAWNSATEATATLFKSLGS 388
              E+    A I + H  LL+D  L +     + +   +A +A  S  +  A  F  +  
Sbjct: 64  LPKESPPELAAILEVHLMLLDDTMLADGVAQWVKDRLYNAEWALTSQLDVVARQFDEMDD 123

Query: 389 TLLAERALDLMDVGQRVLKLILGVPDGV---------------WELPDQA----ILIAEQ 429
             L ER  DL  V +R+L+ + G+P  +                +L D+     +L+A  
Sbjct: 124 PYLRERKGDLEQVVERMLRHMKGMPHVMPQVSPKRSNQPRQQDLQLGDEMDVPLVLVAHD 183

Query: 430 LTPSQTAALDTGKVLGFATVGGGATSHVAILARALGLPAVCGLPLQVLSLASGTRVLLDA 489
           L+P+           GF T  GG TSH AI+AR+L +PAV G       +     +++D 
Sbjct: 184 LSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSLDIPAVVGARTASQLVRQDDWIIIDG 243

Query: 490 DKGELHLDPAVSVIEQLHAKRQQQRQRHQHELENAARAAVTRDGHHFEVTANVASLAETE 549
           D G + +DP+  ++ +   K++Q     +         AVT DG   ++ AN+    ++ 
Sbjct: 244 DAGVVIVDPSPIILAEYGFKQRQAEVERERLSRLRHTPAVTLDGQRVDLLANIEMPEDSP 303

Query: 550 QAMSLGAEGIGLLRSEFLYQQRSVA-PSHDEQAGTYSAIARALGPQRNL--VVRTLDVGG 606
            A+ +GA G+GL RSEFL+  R  A P   EQ   + A  RA+   + L   +RT+DVG 
Sbjct: 304 AALGVGAVGVGLFRSEFLFMGREGALPDEQEQ---FQAYRRAVEGMKGLPVTIRTVDVGA 360

Query: 607 DKPL-AYVPMDSEANPFLGMRGIRLCLERPQLLREQFRAILSSAGLARLHIMLPMVSQLS 665
           DKPL   +  ++  NP LG+R IR  L  P +   Q RAIL +A   ++H+++PM++   
Sbjct: 361 DKPLDRAMKEEAHLNPALGLRAIRWSLADPAMFLTQLRAILRAAAYGQVHLLIPMLAHAR 420

Query: 666 ELRLARLMLEEEALALGLRELP----KLGIMIEVPAAALMADLFAPEVDFFSIGTNDLTQ 721
           E++    +LE+    L  R +     K+G MIEVPAAAL   +F    DF SIGTNDL Q
Sbjct: 421 EIQQTLALLEQARKQLDKRGVAFGPVKVGAMIEVPAAALSVKMFLRYFDFLSIGTNDLIQ 480

Query: 722 YTLAMDRDHPRLASQADSFHPSVLRLIASTVKAAHAHGKWVGVCGALASETLAVPLLLGL 781
           YTLA+DR    +A   D  HP+VLRL+A  +    A GK V VCG +A +     LLLGL
Sbjct: 481 YTLAIDRADESVAHLYDPLHPAVLRLVADVIAECRAQGKGVSVCGEMAGDPSLTRLLLGL 540

Query: 782 GVDELSVSVPLIPAIKAAIREVELSDCQAIAHQVLGLESAEQV 824
           G+   S+    I A+K  I   +    +A +  VL  +   ++
Sbjct: 541 GLRSFSMHPTQILAVKQEIIRADALRLEAWSQSVLASDEPAEL 583


Lambda     K      H
   0.318    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1041
Number of extensions: 51
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 844
Length of database: 586
Length adjustment: 39
Effective length of query: 805
Effective length of database: 547
Effective search space:   440335
Effective search space used:   440335
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources