GapMind for catabolism of small carbon sources

 

Protein WP_109941878.1 in Methanospirillum stamsii Pt1

Annotation: NCBI__GCF_003173335.1:WP_109941878.1

Length: 478 amino acids

Source: GCF_003173335.1 in NCBI

Candidate for 6 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-tryptophan catabolism ecfA1 med Energy-coupling factor transporter ATP-binding protein EcfA1; Short=ECF transporter A component EcfA; EC 7.-.-.- (characterized, see rationale) 41% 78% 160.6 ABC transporter related, component of ThiW/CbiQ/CbiO homologues (ThiW: 174 aas; 5 putative TMSs) 30% 192.6
L-tryptophan catabolism ecfA2 lo Energy-coupling factor transporter ATP-binding protein EcfA2; Short=ECF transporter A component EcfA2; EC 7.-.-.- (characterized, see rationale) 40% 74% 144.4 ABC transporter related, component of ThiW/CbiQ/CbiO homologues (ThiW: 174 aas; 5 putative TMSs) 30% 192.6
D-fructose catabolism frcA lo Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) 32% 86% 109.4 ABC transporter related, component of ThiW/CbiQ/CbiO homologues (ThiW: 174 aas; 5 putative TMSs) 30% 192.6
D-mannose catabolism frcA lo Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) 32% 86% 109.4 ABC transporter related, component of ThiW/CbiQ/CbiO homologues (ThiW: 174 aas; 5 putative TMSs) 30% 192.6
D-ribose catabolism frcA lo Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) 32% 86% 109.4 ABC transporter related, component of ThiW/CbiQ/CbiO homologues (ThiW: 174 aas; 5 putative TMSs) 30% 192.6
sucrose catabolism frcA lo Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) 32% 86% 109.4 ABC transporter related, component of ThiW/CbiQ/CbiO homologues (ThiW: 174 aas; 5 putative TMSs) 30% 192.6

Sequence Analysis Tools

View WP_109941878.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MILASALSYTYPVGVRPALRDITLSISTGELVLITGPTAAGKTTLCYALSGILHHEFGGT
ESGFLSLKNRPIADYAGVAELNQHVSIVFDDADAQLIFTSVEEELASGLETRMDSKEEIE
MRVREVMDLCGISHLKERPPYALSGGQKQRVALAAALAMDTDVIILDEPTSELDVLATTR
IIEILHDLKQKGKTIIIVDHSLEGYRNVADRVVVMGEGMIKKEGTFDLLFPEKQGSMKAL
YDDPDLQPHNPKNRTSIVTINNLVKRYGEIEALRGVSLKFYEGEFIAILGENGSGKTTLV
KHLNGLLRPDSGKITVKLLDAGTAPVMDLVKETGLVFQNPDTMLFADSVRDEILFGLENI
GTENPDLTISEVLSMVSLSGKETIYPRHLSRGERQRLAVACILAMKPEIIILDEPTTGLD
EKESDRMMELMLTLQKTGHTIIMVTHNMRIAEMYADRIIAMENGRVIGDYHNLKRMAS

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory