Protein WP_109970082.1 in Methanospirillum lacunae Ki8-1
Annotation: NCBI__GCF_003173355.1:WP_109970082.1
Length: 251 amino acids
Source: GCF_003173355.1 in NCBI
Candidate for 10 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-histidine catabolism | Ac3H11_2560 | med | ABC transporter for L-Histidine, ATPase component (characterized) | 41% | 80% | 166.8 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| L-histidine catabolism | hutV | med | ABC transporter for L-Histidine, ATPase component (characterized) | 42% | 78% | 151.4 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| putrescine catabolism | potA | lo | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) | 45% | 51% | 161 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| L-arabinose catabolism | xacJ | lo | Xylose/arabinose import ATP-binding protein XacJ; EC 7.5.2.13 (characterized, see rationale) | 40% | 54% | 154.1 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| L-proline catabolism | opuBA | lo | BilEA aka OpuBA protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) (characterized) | 40% | 63% | 151.4 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| N-acetyl-D-glucosamine catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 40% | 62% | 150.2 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| D-glucosamine (chitosamine) catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 40% | 62% | 150.2 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| D-maltose catabolism | malK_Sm | lo | MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) | 38% | 56% | 149.8 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| trehalose catabolism | malK | lo | MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) | 38% | 56% | 149.8 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
| D-maltose catabolism | malK | lo | Maltose-transporting ATPase (EC 3.6.3.19) (characterized) | 34% | 60% | 137.1 | Aliphatic sulfonates import ATP-binding protein SsuB; EC 7.6.2.- | 44% | 197.6 |
Sequence Analysis Tools
View WP_109970082.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MNEVLVVDNISKQFIDDDSVCRAIDSLSLTIRKKEIVCLMGPSGCGKSTVLRIISKLEHA
DSGQIHGGDGVFSEQLRSAMVFQEHGLFPWLSVRENIAYGLNMKVRFSSKEQVNQKVTEL
LTLVRMEEFANSHPHQLSGGMKQRVAVARALAVEPEILLMDEPFSALDPFTRRELQDEVL
RIRNQLNTTFFIVTHNPEEAVYLSDRIVILTHRPAVVRKEIPVSLPFPRNMADPSTIALV
QDVTRLVASGT
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory