Align Histidine transport system permease protein HisQ (characterized)
to candidate WP_109969949.1 DK846_RS15745 amino acid ABC transporter permease
Query= SwissProt::P52094 (228 letters) >NCBI__GCF_003173355.1:WP_109969949.1 Length = 218 Score = 102 bits (254), Expect = 6e-27 Identities = 62/212 (29%), Positives = 110/212 (51%), Gaps = 15/212 (7%) Query: 12 GALVTLELAISSVVLAVIIGLIGAGGKLSQNRLSGLIFEGYTTLIRGVPDLVLMLLIFYG 71 G LVTL L + + +G+I A G++ ++ IF+GYT +G P L+L+ ++++G Sbjct: 18 GLLVTLSLIAVTAPIGFALGIIIACGRVYGGKVVSKIFQGYTIFFKGCPLLLLLFILYFG 77 Query: 72 LQIALNTVTEAMGVGQIDIDPMVAGIITLGFIYGAYFTETFRGAFMAVPKGHIEAATAFG 131 L I + P VA +I AY +E RGA ++V +G I AA A G Sbjct: 78 LPPY-----------GITLSPFVASVIGFILCNSAYNSEYVRGAILSVKEGQITAAKALG 126 Query: 132 FTRGQVFRRIMFPSMMRYALPGIGNNWQVILKSTALVSLLGLEDVVKATQLAGKS--TWE 189 TR Q R ++ P +R A+PGI N + ++K ++L ++ + ++ A +L + Sbjct: 127 MTRNQAIRFVVLPQALRRAIPGISNEFIYLIKYSSLAYMITVIELTGAGKLIATKYFAYN 186 Query: 190 PFYFAIVCGVIYLVFTTVSNGVLLFLERRYSV 221 +FA+ ++YL T++ +ER++S+ Sbjct: 187 ETFFAL--AIVYLALVTITTFGANAIERKFSI 216 Lambda K H 0.328 0.145 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 148 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 228 Length of database: 218 Length adjustment: 22 Effective length of query: 206 Effective length of database: 196 Effective search space: 40376 Effective search space used: 40376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory