GapMind for catabolism of small carbon sources

 

Alignments for a candidate for potA in Methanospirillum lacunae Ki8-1

Align spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized)
to candidate WP_109970082.1 DK846_RS16450 ABC transporter ATP-binding protein

Query= CharProtDB::CH_024626
         (378 letters)



>NCBI__GCF_003173355.1:WP_109970082.1
          Length = 251

 Score =  159 bits (402), Expect = 8e-44
 Identities = 87/191 (45%), Positives = 127/191 (66%), Gaps = 6/191 (3%)

Query: 33  IPQLDLTINNGEFLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVN 92
           I  L LTI   E + L+GPSGCGK+TVLR+I+ LE  DSG+I   +     V +E     
Sbjct: 24  IDSLSLTIRKKEIVCLMGPSGCGKSTVLRIISKLEHADSGQIHGGDG----VFSEQLRSA 79

Query: 93  TVFQSYALFPHMTVFENVAFGLRMQK--TPAAEITPRVMEALRMVQLETFAQRKPHQLSG 150
            VFQ + LFP ++V EN+A+GL M+   +   ++  +V E L +V++E FA   PHQLSG
Sbjct: 80  MVFQEHGLFPWLSVRENIAYGLNMKVRFSSKEQVNQKVTELLTLVRMEEFANSHPHQLSG 139

Query: 151 GQQQRVAIARAVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEE 210
           G +QRVA+ARA+  +P +LL+DE  SALD   R+++Q+E+  ++ +L  TF  VTH+ EE
Sbjct: 140 GMKQRVAVARALAVEPEILLMDEPFSALDPFTRRELQDEVLRIRNQLNTTFFIVTHNPEE 199

Query: 211 ALTMSDRIVVM 221
           A+ +SDRIV++
Sbjct: 200 AVYLSDRIVIL 210


Lambda     K      H
   0.319    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 206
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 378
Length of database: 251
Length adjustment: 27
Effective length of query: 351
Effective length of database: 224
Effective search space:    78624
Effective search space used:    78624
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory