GapMind for catabolism of small carbon sources

 

Protein WP_110207906.1 in Nocardioides daejeonensis MJ31

Annotation: NCBI__GCF_003194585.1:WP_110207906.1

Length: 737 amino acids

Source: GCF_003194585.1 in NCBI

Candidate for 22 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism fadB med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
4-hydroxybenzoate catabolism paaH med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
L-arginine catabolism fadB med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
L-citrulline catabolism fadB med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
L-isoleucine catabolism ech med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379
L-lysine catabolism fadB med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
phenylacetate catabolism fadB med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
phenylacetate catabolism paaH med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
L-phenylalanine catabolism fadB med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
L-phenylalanine catabolism paaH med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
L-proline catabolism fadB med fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized) 36% 97% 379 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 32% 335.9
4-hydroxybenzoate catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 31% 96% 271.9 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 36% 379.0
phenylacetate catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 31% 96% 271.9 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 36% 379.0
L-phenylalanine catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 31% 96% 271.9 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 36% 379.0
4-hydroxybenzoate catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 58% 167.9
L-arginine catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 58% 167.9
L-citrulline catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 58% 167.9
L-lysine catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 58% 167.9
phenylacetate catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 58% 167.9
L-phenylalanine catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 58% 167.9
L-proline catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 58% 167.9
L-valine catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 58% 167.9

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Predict transmenbrane helices: Phobius

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Sequence

MARPAPDRGEEVSTESSSASIITWSRDEDGVVVVTLDDPTQEVNTVHTGFVHELGALVEH
LQAEREGLAGVVITSAKSTFVAGGDLNEIVTLGLADAARFTRHLNNLKATLRTLETLGVP
VVAAINGSALGGGLELALAAHHRIVVDSPRVQLGLPEVGLGLLPASGGIVRTVRMLGAQR
ALDEVLLSGRRFRPTQARDLGLVDELVDDVADLLPRAKAWIAEHPDATQPWDVKGFRFPG
GTQASGALASGLPFLASTLRAKTGGAPAPAQRAILAAAVEGVQVDIDAAGLIETRYFIEI
ATAPISKNLINLTFFDMQEIKGGASRPDGFPKSRVQKVGVVGAGMMGAGIAYAAARAGID
VVLMDTTLAGAEKGRAYSAKVEGRAVARGQRTQEAADQVVARITATDDVQDLAGVDFVIE
AVFENAALKAEVFASVEQVVGAEAVLGSNTSTLPISSLAAGVARPQDFVGVHFFSPVDRM
PLVEIIRGEATSDATLAKAFDLVLQLGKTPIVVNDSRGFFTSRVIIARLNEAVAMVGEGV
PCPSIEQASLQAGYPAGALQLLDELTLTLPRAVREEAKAAAEAAGLDWLAHPSHAIFDRL
IDEFGRVGRAGGGGFYDYDDQGKRTGFWPGLASSFPTLDTMPPLPDLVDRLLFAEVLEAW
AAHVAGVIEAPSDANVGSLLGIGFPAWTGGVLRFLDQYDGGRAGFVARAEQLAARYGDRF
LPPATLLETLERVERIA

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory