Alignments for a candidate for paaH in Nocardioides daejeonensis MJ31

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaH in Nocardioides daejeonensis MJ31

Align fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized)
to candidate WP_110207906.1 DNK54_RS14955 3-hydroxyacyl-CoA dehydrogenase

Query= CharProtDB::CH_003230
         (714 letters)



>NCBI__GCF_003194585.1:WP_110207906.1
          Length = 737

 Score =  364 bits (934), Expect = e-104
 Identities = 258/716 (36%), Positives = 379/716 (52%), Gaps = 36/716 (5%)

Query: 14  DNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKE-LRGVVFVSAKPDNFIAGADIN 72
           D + V+T+D P +++NT+   F  ++ A+++ L+  +E L GVV  SAK   F+AG D+N
Sbjct: 29  DGVVVVTLDDPTQEVNTVHTGFVHELGALVEHLQAEREGLAGVVITSAK-STFVAGGDLN 87

Query: 73  MIGNCKTAQEAEALARQGQQLMAEIHALP---IQVIAAIHGACLGGGLELALACHGRVCT 129
            I     A +A    R    L A +  L    + V+AAI+G+ LGGGLELALA H R+  
Sbjct: 88  EIVTLGLA-DAARFTRHLNNLKATLRTLETLGVPVVAAINGSALGGGLELALAAHHRIVV 146

Query: 130 DDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTAL-EMILTGKQLRAKQALKLGLVDDV 188
           D P+  LGLPEV LGLLP SGG  R  R++G   AL E++L+G++ R  QA  LGLVD++
Sbjct: 147 DSPRVQLGLPEVGLGLLPASGGIVRTVRMLGAQRALDEVLLSGRRFRPTQARDLGLVDEL 206

Query: 189 VPH-SILLEAAVELAKKERPSSRPLPVRE------RILAGPLGRALLFKMVGKKTEHKTQ 241
           V   + LL  A     +   +++P  V+          +G L   L F  +      KT 
Sbjct: 207 VDDVADLLPRAKAWIAEHPDATQPWDVKGFRFPGGTQASGALASGLPF--LASTLRAKTG 264

Query: 242 GN-YPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSI-FFASTDVKKDP 299
           G   PA   IL     G+     +    E R F E+A  P S+ L ++ FF   ++K   
Sbjct: 265 GAPAPAQRAILAAAVEGVQVDIDAAGLIETRYFIEIATAPISKNLINLTFFDMQEIKGGA 324

Query: 300 G--SDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLE 357
                 P + +  VG++G G+MG GIAY  A +AGI V + D    G      YS  ++E
Sbjct: 325 SRPDGFPKSRVQKVGVVGAGMMGAGIAYAAA-RAGIDVVLMDTTLAGAEKGRAYS-AKVE 382

Query: 358 GKVRRRHLKASER-DKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAA 416
           G+   R  +  E  D+ +A I+ T D +  A  D +IEAVFEN  LK ++ A VEQ   A
Sbjct: 383 GRAVARGQRTQEAADQVVARITATDDVQDLAGVDFVIEAVFENAALKAEVFASVEQVVGA 442

Query: 417 HTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKL 476
             +  SNTS+LPI  +AA   RP+  +G+HFFSPV++MPLVEII    TS  T+A    L
Sbjct: 443 EAVLGSNTSTLPISSLAAGVARPQDFVGVHFFSPVDRMPLVEIIRGEATSDATLAKAFDL 502

Query: 477 AKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLL 536
             + GKTPIVV D  GF+ +R++   +NEA+ M+ +G     I+ A ++ G+P G +QLL
Sbjct: 503 VLQLGKTPIVVNDSRGFFTSRVIIARLNEAVAMVGEGVPCPSIEQASLQAGYPAGALQLL 562

Query: 537 DEVGIDTGTKI----IPVLEAAYGERFSAPANVVSSILNDD--RKGRKNGRGFYLYGQKG 590
           DE+ +     +        EAA  +  + P++ +   L D+  R GR  G GFY Y  +G
Sbjct: 563 DELTLTLPRAVREEAKAAAEAAGLDWLAHPSHAIFDRLIDEFGRVGRAGGGGFYDYDDQG 622

Query: 591 RKSKKQVDPA-IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGA 649
           +++      A  +P + T        P + +R +   + EA       VI +  D ++G+
Sbjct: 623 KRTGFWPGLASSFPTLDTMP----PLPDLVDRLLFAEVLEAWAAHVAGVIEAPSDANVGS 678

Query: 650 VFGIGFPPFLGGPFRYIDSLGAGEV--VAIMQRLATQYGSRFTPCERLVEMGARGE 703
           + GIGFP + GG  R++D    G    VA  ++LA +YG RF P   L+E   R E
Sbjct: 679 LLGIGFPAWTGGVLRFLDQYDGGRAGFVARAEQLAARYGDRFLPPATLLETLERVE 734


Lambda     K      H
   0.319    0.137    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1108
Number of extensions: 54
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 714
Length of database: 737
Length adjustment: 40
Effective length of query: 674
Effective length of database: 697
Effective search space:   469778
Effective search space used:   469778
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources