Potential Gaps in catabolism of small carbon sources in Phyllobacterium leguminum ORS 1419
Found 74 low-confidence and 58 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| 2-oxoglutarate | kgtP: 2-oxoglutarate:H+ symporter KgtP | C7477_RS03870 | C7477_RS03325 |
| 4-hydroxybenzoate | adh: acetaldehyde dehydrogenase (not acylating) | C7477_RS02030 | C7477_RS17335 |
| 4-hydroxybenzoate | mhpD: 2-hydroxypentadienoate hydratase | | |
| 4-hydroxybenzoate | mhpE: 4-hydroxy-2-oxovalerate aldolase | C7477_RS08100 | |
| 4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
| 4-hydroxybenzoate | pobA: 4-hydroxybenzoate 3-monooxygenase | | |
| 4-hydroxybenzoate | praA: protocatechuate 2,3-dioxygenase | | |
| 4-hydroxybenzoate | xylF: 2-hydroxymuconate semialdehyde hydrolase | | |
| alanine | braC: L-alanine/L-serine/L-threonine ABC transporter, substrate binding protein (BraC/NatB) | C7477_RS09150 | C7477_RS01685 |
| alanine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | C7477_RS09175 | |
| alanine | braE: L-alanine/L-serine/L-threonine ABC transporter, permease component 2 (BraE/NatC) | C7477_RS09170 | |
| alanine | braF: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 1 (BraF/NatA) | C7477_RS09165 | C7477_RS05360 |
| arabinose | xacB: L-arabinose 1-dehydrogenase | C7477_RS04415 | C7477_RS09435 |
| arabinose | xacD: L-arabinonate dehydratase | C7477_RS13890 | C7477_RS13745 |
| cellobiose | cbp: cellobiose phosphorylase | | |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | C7477_RS15120 | C7477_RS12950 |
| citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | C7477_RS15125 | C7477_RS09135 |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | C7477_RS15130 | C7477_RS09130 |
| citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | C7477_RS09125 | C7477_RS09120 |
| citrulline | arcC: carbamate kinase | | |
| D-alanine | dadA: D-alanine dehydrogenase | C7477_RS04965 | |
| D-alanine | Pf6N2E2_5402: ABC transporter for D-Alanine, substrate-binding component | C7477_RS12965 | |
| D-alanine | Pf6N2E2_5403: ABC transporter for D-Alanine, permease component 2 | C7477_RS12960 | |
| D-alanine | Pf6N2E2_5404: ABC transporter for D-Alanine, permease component 1 | C7477_RS12955 | C7477_RS12390 |
| D-alanine | Pf6N2E2_5405: ABC transporter for D-Alanine, ATPase component | C7477_RS12950 | C7477_RS15120 |
| D-serine | cycA: D-serine:H+ symporter CycA | C7477_RS13980 | |
| D-serine | dsdA: D-serine ammonia-lyase | C7477_RS02530 | |
| deoxyinosine | adh: acetaldehyde dehydrogenase (not acylating) | C7477_RS02030 | C7477_RS17335 |
| deoxyinosine | nupC: deoxyinosine:H+ symporter NupC | | |
| deoxyribonate | aacS: acetoacetyl-CoA synthetase | C7477_RS15250 | |
| deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | C7477_RS06910 | C7477_RS09440 |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | | |
| deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | | |
| deoxyribose | adh: acetaldehyde dehydrogenase (not acylating) | C7477_RS02030 | C7477_RS17335 |
| deoxyribose | deoP: deoxyribose transporter | | |
| ethanol | adh: acetaldehyde dehydrogenase (not acylating) | C7477_RS02030 | C7477_RS17335 |
| ethanol | etoh-dh-nad: ethanol dehydrogenase (NAD(P)) | C7477_RS17345 | C7477_RS02270 |
| galactose | dgoD: D-galactonate dehydratase | C7477_RS13890 | C7477_RS13745 |
| galacturonate | exuT: D-galacturonate transporter ExuT | | |
| gluconate | gntB: gluconate TRAP transporter, large permease component | C7477_RS02260 | C7477_RS15555 |
| glucosamine | nagX: transmembrane glucosamine N-acetyltransferase NagX | | |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glucuronate | dctQ: D-glucuronate TRAP transporter, small permease component | C7477_RS00810 | |
| isoleucine | acdH: (2S)-2-methylbutanoyl-CoA dehydrogenase | C7477_RS01300 | |
| isoleucine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | C7477_RS16445 | |
| isoleucine | bkdB: branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit | C7477_RS16440 | |
| isoleucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | C7477_RS05725 | C7477_RS16435 |
| isoleucine | ivdG: 3-hydroxy-2-methylbutyryl-CoA dehydrogenase | C7477_RS17870 | C7477_RS08275 |
| isoleucine | pccA: propionyl-CoA carboxylase, alpha subunit | C7477_RS09800 | C7477_RS08875 |
| isoleucine | pccB: propionyl-CoA carboxylase, beta subunit | | |
| L-malate | sdlC: L-malate:Na+ symporter SdlC | | |
| lactose | dgoD: D-galactonate dehydratase | C7477_RS13890 | C7477_RS13745 |
| lactose | lacP: lactose permease LacP | | |
| lactose | lacZ: lactase (homomeric) | | |
| leucine | aacS: acetoacetyl-CoA synthetase | C7477_RS15250 | |
| leucine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | C7477_RS16445 | |
| leucine | bkdB: branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit | C7477_RS16440 | |
| leucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | C7477_RS05725 | C7477_RS16435 |
| leucine | liuB: 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit | C7477_RS09800 | C7477_RS17935 |
| leucine | liuC: 3-methylglutaconyl-CoA hydratase | C7477_RS04670 | C7477_RS16650 |
| leucine | liuD: 3-methylcrotonyl-CoA carboxylase, beta subunit | | |
| leucine | liuE: hydroxymethylglutaryl-CoA lyase | | |
| lysine | lysN: 2-aminoadipate transaminase | C7477_RS15835 | C7477_RS05570 |
| lysine | ydiJ: (R)-2-hydroxyglutarate dehydrogenase | C7477_RS01285 | |
| maltose | susB: alpha-glucosidase (maltase) | | |
| mannitol | mt2d: mannitol 2-dehydrogenase | C7477_RS15550 | C7477_RS09435 |
| mannitol | mtlG: polyol ABC transporter, permease component 2 (MtlG/SmoG) | C7477_RS09450 | C7477_RS13715 |
| mannose | man-isomerase: D-mannose isomerase | | |
| myoinositol | iolC: 5-dehydro-2-deoxy-D-gluconate kinase | | |
| myoinositol | iolJ: 5-dehydro-2-deoxyphosphogluconate aldolase | | |
| myoinositol | PGA1_c07300: myo-inositol ABC transport, substrate-binding component | C7477_RS15705 | |
| phenylacetate | paaA: phenylacetyl-CoA 1,2-epoxidase, subunit A | | |
| phenylacetate | paaB: phenylacetyl-CoA 1,2-epoxidase, subunit B | | |
| phenylacetate | paaC: phenylacetyl-CoA 1,2-epoxidase, subunit C | | |
| phenylacetate | paaE: phenylacetyl-CoA 1,2-epoxidase, subunit E | | |
| phenylacetate | paaG: 1,2-epoxyphenylacetyl-CoA isomerase / 2-(oxepinyl)acetyl-CoA isomerase / didehydroadipyl-CoA isomerase | C7477_RS04670 | |
| phenylacetate | paaH: 3-hydroxyadipyl-CoA dehydrogenase | C7477_RS16650 | C7477_RS05975 |
| phenylacetate | paaJ1: 3-oxo-5,6-dehydrosuberyl-CoA thiolase | C7477_RS17875 | C7477_RS16645 |
| phenylacetate | paaJ2: 3-oxoadipyl-CoA thiolase | C7477_RS17875 | C7477_RS16645 |
| phenylacetate | paaK: phenylacetate-CoA ligase | | |
| phenylacetate | paaT: phenylacetate transporter Paa | | |
| phenylacetate | paaZ1: oxepin-CoA hydrolase | C7477_RS04670 | |
| phenylacetate | paaZ2: 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase | | |
| phenylalanine | aacS: acetoacetyl-CoA synthetase | C7477_RS15250 | |
| phenylalanine | fahA: fumarylacetoacetate hydrolase | C7477_RS09525 | C7477_RS14915 |
| phenylalanine | hmgA: homogentisate dioxygenase | | |
| phenylalanine | HPD: 4-hydroxyphenylpyruvate dioxygenase | | |
| phenylalanine | livH: L-phenylalanine ABC transporter, permease component 1 (LivH) | C7477_RS09175 | C7477_RS12430 |
| phenylalanine | livJ: L-phenylalanine ABC transporter, substrate-binding component LivJ/LivK | C7477_RS09150 | C7477_RS01685 |
| phenylalanine | maiA: maleylacetoacetate isomerase | C7477_RS11235 | |
| phenylalanine | PAH: phenylalanine 4-monooxygenase | | |
| phenylalanine | PCBD: pterin-4-alpha-carbinoalamine dehydratase | | |
| phenylalanine | QDPR: 6,7-dihydropteridine reductase | C7477_RS13920 | |
| propionate | pccA: propionyl-CoA carboxylase, alpha subunit | C7477_RS09800 | C7477_RS08875 |
| propionate | pccB: propionyl-CoA carboxylase, beta subunit | | |
| putrescine | gabD: succinate semialdehyde dehydrogenase | C7477_RS02030 | C7477_RS17335 |
| putrescine | patA: putrescine aminotransferase (PatA/SpuC) | C7477_RS13250 | C7477_RS13210 |
| putrescine | patD: gamma-aminobutyraldehyde dehydrogenase | C7477_RS17335 | C7477_RS02030 |
| putrescine | potD: putrescine ABC transporter, substrate-binding component (PotD/PotF) | C7477_RS00140 | |
| serine | braC: L-alanine/L-serine/L-threonine ABC transporter, substrate binding protein (BraC/NatB) | C7477_RS09150 | C7477_RS01685 |
| serine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | C7477_RS09175 | |
| serine | braE: L-alanine/L-serine/L-threonine ABC transporter, permease component 2 (BraE/NatC) | C7477_RS09170 | |
| serine | braF: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 1 (BraF/NatA) | C7477_RS09165 | C7477_RS05360 |
| succinate | sdc: succinate:Na+ symporter Sdc | | |
| sucrose | SUS: sucrose synthase | | |
| threonine | braC: L-alanine/L-serine/L-threonine ABC transporter, substrate binding protein (BraC/NatB) | C7477_RS09150 | C7477_RS01685 |
| threonine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | C7477_RS09175 | |
| threonine | braE: L-alanine/L-serine/L-threonine ABC transporter, permease component 2 (BraE/NatC) | C7477_RS09170 | |
| threonine | braF: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 1 (BraF/NatA) | C7477_RS09165 | C7477_RS05360 |
| threonine | tdh: L-threonine 3-dehydrogenase | C7477_RS10800 | C7477_RS15570 |
| threonine | tynA: aminoacetone oxidase | | |
| thymidine | adh: acetaldehyde dehydrogenase (not acylating) | C7477_RS02030 | C7477_RS17335 |
| thymidine | nupG: thymidine permease NupG/XapB | | |
| trehalose | PsTP: trehalose phosphorylase | | |
| tryptophan | aroP: tryptophan:H+ symporter AroP | C7477_RS13980 | |
| tryptophan | tnaA: tryptophanase | | |
| tyrosine | aacS: acetoacetyl-CoA synthetase | C7477_RS15250 | |
| tyrosine | aroP: L-tyrosine transporter (AroP/FywP) | C7477_RS13980 | |
| tyrosine | fahA: fumarylacetoacetate hydrolase | C7477_RS09525 | C7477_RS14915 |
| tyrosine | hmgA: homogentisate dioxygenase | | |
| tyrosine | HPD: 4-hydroxyphenylpyruvate dioxygenase | | |
| tyrosine | maiA: maleylacetoacetate isomerase | C7477_RS11235 | |
| valine | acdH: isobutyryl-CoA dehydrogenase | C7477_RS16640 | C7477_RS01300 |
| valine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | C7477_RS16445 | |
| valine | bkdB: branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit | C7477_RS16440 | |
| valine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | C7477_RS05725 | C7477_RS16435 |
| valine | mmsA: methylmalonate-semialdehyde dehydrogenase | C7477_RS15650 | C7477_RS02030 |
| valine | mmsB: 3-hydroxyisobutyrate dehydrogenase | C7477_RS16460 | |
| valine | pccA: propionyl-CoA carboxylase, alpha subunit | C7477_RS09800 | C7477_RS08875 |
| valine | pccB: propionyl-CoA carboxylase, beta subunit | | |
| xylitol | PLT5: xylitol:H+ symporter PLT5 | | |
| xylitol | xdhA: xylitol dehydrogenase | C7477_RS15570 | C7477_RS10275 |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Links
Downloads
Related tools
About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory