GapMind for catabolism of small carbon sources

 

Protein WP_110751803.1 in Phyllobacterium leguminum ORS 1419

Annotation: NCBI__GCF_003217235.1:WP_110751803.1

Length: 333 amino acids

Source: GCF_003217235.1 in NCBI

Candidate for 11 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
N-acetyl-D-glucosamine catabolism SMc02869 hi N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) 76% 94% 500.7 SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter 59% 396.0
D-glucosamine (chitosamine) catabolism SMc02869 hi N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) 76% 94% 500.7 SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter 59% 396.0
D-mannitol catabolism mtlK med SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter (characterized) 59% 100% 396 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7
D-sorbitol (glucitol) catabolism mtlK med ABC transporter for D-Sorbitol, ATPase component (characterized) 60% 99% 393.7 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7
D-maltose catabolism thuK med ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) 57% 98% 380.2 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7
sucrose catabolism thuK med ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) 57% 98% 380.2 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7
trehalose catabolism thuK med ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) 57% 98% 380.2 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7
xylitol catabolism Dshi_0546 med ABC transporter for Xylitol, ATPase component (characterized) 52% 100% 334.7 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7
L-histidine catabolism natE lo NatE aka LivF aka SLR1881, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) 33% 88% 107.5 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7
L-leucine catabolism natE lo NatE aka LivF aka SLR1881, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) 33% 88% 107.5 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7
L-proline catabolism natE lo NatE aka LivF aka SLR1881, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) 33% 88% 107.5 N-Acetyl-D-glucosamine ABC transport system, ATPase component 76% 500.7

Sequence Analysis Tools

View WP_110751803.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MGTLKLENIRKSFGSVDVLKGIDLDVKDGELVVFVGPSGCGKSTLLRIIAGLEDATSGDV
LIDGAAAGHIPPAKRGIAMVFQTYALYPHLTVRDNMGLGLKQAGTPAAEIAGRVGKASAM
LSLDDYLARRPAELSGGQRQRVAIGRALVREPKLFLFDEPLSNLDAALRVNTRLEIARLH
RELSATMIYVTHDQVEAMTLADKIVVLNNGRIEQIGAPMELYNSPANLFVAGFIGSPKMN
FIDAARLGHEGVKTIGVRPEHITVDPTSGTWAGSVMHVEHLGPDTNLYLDSKEAGLITIR
LFGEYRVEPGAALYATPDPARTYRFDAAGQVIA

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory