GapMind for catabolism of small carbon sources

 

Alignments for a candidate for artM in Rhodobacter viridis JA737

Align Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate WP_110806870.1 C8J30_RS16145 ABC transporter permease subunit

Query= TCDB::Q9HU29
         (230 letters)



>NCBI__GCF_003217355.1:WP_110806870.1
          Length = 232

 Score =  177 bits (450), Expect = 1e-49
 Identities = 103/232 (44%), Positives = 141/232 (60%), Gaps = 4/232 (1%)

Query: 1   MSEWELILKWMPKMLQGAALTLELLAIAVVAGLALALPLGIARASRHWYVRAVPYAYIFF 60
           MS  E +   +PK+L GA  TL L  +        +  L + R       + +  AYI F
Sbjct: 1   MSLIESLSTNLPKLLDGAETTLILTGLGAALAAVFSPGLALIRLFGPTPAKMLLRAYISF 60

Query: 61  FRGTPLLLQLFIVYYGLAQFE-EVRKSAFWPYLRDPYWCALLTMTLHTAAYIAEILRGAI 119
            RGTPLL QLF+V+YG  QF  E+     W + RDP+ CA+L   +++ AY  EILRG I
Sbjct: 61  VRGTPLLAQLFLVFYGSGQFRAELTGLGLWSFFRDPFNCAVLVFVINSCAYQTEILRGGI 120

Query: 120 HSVPVGEVEAARALGMSRRQALWHIILPRAVRIGLPAYSNEVILMLKASAVVYTVTLFDI 179
            +VP GE+EAARA+GM+R + L  +I P A RI  PA  NEVIL++KASA+   VT+FD+
Sbjct: 121 EAVPPGEIEAARAIGMTRARVLARVIFPHAYRIAWPALGNEVILLMKASALASIVTVFDL 180

Query: 180 MGMARTIIARTYESMLFFCLAGALYLVITIVLTRIFRLIERWL--RVDATQG 229
           MG  R I +R++E  ++F  A ALYL+IT+V   ++R IER L   +  TQG
Sbjct: 181 MGRTRQIFSRSFEFSVYF-EAAALYLLITVVFVFLWRQIERRLTRHMRRTQG 231


Lambda     K      H
   0.332    0.142    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 147
Number of extensions: 8
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 230
Length of database: 232
Length adjustment: 23
Effective length of query: 207
Effective length of database: 209
Effective search space:    43263
Effective search space used:    43263
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory